Detailed information of TA system TA112534

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): symER/SymE(toxin)
• Location: 4882784..4883196
• Replicon: chromosome
• Accession: NZ_CP032808
• Organism: Escherichia coli strain ERL04-3476

Toxin (Protein)

• Gene name: symE
• Uniprot ID: Q8FA88
• Locus tag: D8Z68_RS25150
• Protein ID: WP_000132630.1
• Coordinates: 4882855..4883196 (+)
• Length: 114 a.a.

Antitoxin (RNA)

Gene name	symR
Locus tag	-
Coordinates	4882784..4882860 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
D8Z68_RS25140	4879411..4880880	+	1470	WP_001302468.1	type I restriction-modification system subunit M	-
D8Z68_RS25145	4880880..4882634	+	1755	WP_000110082.1	restriction endonuclease subunit S	-
-	4882784..4882860	-	77	NuclAT_14	-	Antitoxin
-	4882784..4882860	-	77	NuclAT_14	-	Antitoxin
-	4882784..4882860	-	77	NuclAT_14	-	Antitoxin
-	4882784..4882860	-	77	NuclAT_14	-	Antitoxin
-	4882784..4882860	-	77	NuclAT_15	-	Antitoxin
-	4882784..4882860	-	77	NuclAT_15	-	Antitoxin
-	4882784..4882860	-	77	NuclAT_15	-	Antitoxin
-	4882784..4882860	-	77	NuclAT_15	-	Antitoxin
D8Z68_RS25150	4882855..4883196	+	342	WP_000132630.1	endoribonuclease SymE	Toxin
D8Z68_RS25155	4883243..4884406	-	1164	WP_001304006.1	DUF1524 domain-containing protein	-
D8Z68_RS25160	4884454..4885335	-	882	WP_001304007.1	DUF262 domain-containing protein	-
D8Z68_RS25165	4885432..4885596	-	165	WP_000394274.1	DUF1127 domain-containing protein	-
D8Z68_RS25170	4885773..4887185	+	1413	WP_000199295.1	PLP-dependent aminotransferase family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin SymE-like (20-71)

Sequences

Toxin

Protein

• Length: 114 a.a.
• Molecular weight: 12294.13 Da
• Isoelectric Point: 8.4982

>T112534 WP_000132630.1 NZ_CP032808:4882855-4883196 [Escherichia coli]
MTDTHSIAQPFEAEVSPANNRQLTVSYASRYPDYSRIPAITLKGQWLEAAGFATGTVVDVKVMEGCIVLTAQPPAAAESE
LMQSLRQVCKLSARKQRQVQEFIGVIAGKQKVA

Nucleotide

• Length: 342 bp

>T112534 NZ_CP032808:4882855-4883196 [Escherichia coli]
ATGACTGATACGCATTCTATTGCACAACCGTTCGAAGCAGAAGTCTCCCCGGCAAATAACCGTCAATTAACCGTGAGTTA
TGCGAGTCGTTACCCGGATTACAGCCGTATTCCCGCCATCACCCTGAAAGGTCAGTGGCTGGAAGCCGCCGGTTTTGCCA
CCGGCACGGTGGTAGATGTCAAAGTGATGGAAGGCTGTATTGTCCTCACCGCCCAACCACCCGCCGCCGCCGAGAGCGAA
CTGATGCAGTCGCTGCGCCAGGTGTGCAAGCTGTCGGCGCGTAAACAAAGGCAGGTGCAGGAGTTTATTGGGGTGATTGC
GGGTAAACAGAAAGTTGCCTGA

Antitoxin

Nucleotide

• Length: 77 bp

>AT112534 NZ_CP032808:c4882860-4882784 [Escherichia coli]
AGTCATAACTGCTATTCCCTATAAATAGTGATTGTGATTAGCGGTGCGGGTGTGTTGGCGCACATCCGCACCGCGCT

Structures

Toxin

• AlphaFold DB: A0A0E0Y5T0

Antitoxin