Detailed information of TA system TA112522

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 2901056..2901281
• Replicon: chromosome
• Accession: NZ_CP032808
• Organism: Escherichia coli strain ERL04-3476

Toxin (Protein)

• Gene name: hokW
• Uniprot ID: A0A1Q4PF16
• Locus tag: D8Z68_RS15310
• Protein ID: WP_000813258.1
• Coordinates: 2901126..2901281 (+)
• Length: 52 a.a.

Antitoxin (RNA)

Gene name	sokW
Locus tag	-
Coordinates	2901056..2901114 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
D8Z68_RS15260	2896121..2896363	+	243	WP_000747948.1	hypothetical protein	-
D8Z68_RS15265	2896347..2896772	+	426	WP_151547382.1	Rha family transcriptional regulator	-
D8Z68_RS15270	2896841..2897884	+	1044	WP_001262402.1	hypothetical protein	-
D8Z68_RS15275	2897877..2898338	+	462	WP_000139447.1	replication protein	-
D8Z68_RS15280	2898372..2899088	+	717	WP_000450627.1	DUF1627 domain-containing protein	-
D8Z68_RS15285	2899121..2899402	+	282	WP_000603384.1	DNA-binding protein	-
D8Z68_RS15290	2899399..2899626	+	228	WP_000699809.1	hypothetical protein	-
D8Z68_RS15295	2899619..2899930	+	312	WP_001289673.1	hypothetical protein	-
D8Z68_RS15300	2900058..2900276	+	219	WP_000683609.1	DUF4014 family protein	-
D8Z68_RS15305	2900278..2900835	+	558	WP_000104474.1	DUF551 domain-containing protein	-
-	2901056..2901114	-	59	-	-	Antitoxin
D8Z68_RS15310	2901126..2901281	+	156	WP_000813258.1	type I toxin-antitoxin system Hok family toxin	Toxin
D8Z68_RS15315	2901401..2901745	+	345	WP_000756596.1	YgiW/YdeI family stress tolerance OB fold protein	-
D8Z68_RS15320	2901867..2902139	+	273	WP_000191872.1	hypothetical protein	-
D8Z68_RS15325	2902141..2903190	+	1050	WP_001265229.1	DUF968 domain-containing protein	-
D8Z68_RS15330	2903203..2903508	+	306	WP_001217444.1	RusA family crossover junction endodeoxyribonuclease	-
D8Z68_RS15335	2903571..2904125	+	555	WP_000640035.1	DUF1133 family protein	-
D8Z68_RS15340	2904350..2904547	+	198	WP_000917763.1	hypothetical protein	-
D8Z68_RS15345	2904683..2905396	+	714	WP_000301797.1	helix-turn-helix domain-containing protein	-
D8Z68_RS15360	2905847..2906278	+	432	WP_001302123.1	tellurite resistance protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	2895391..2941036	45645

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (6-47)

Sequences

Toxin

Protein

• Length: 52 a.a.
• Molecular weight: 5767.95 Da
• Isoelectric Point: 7.7942

>T112522 WP_000813258.1 NZ_CP032808:2901126-2901281 [Escherichia coli]
MKQQKAMLIALIVICLTVIVTALVTRKDLCEVRIRTGQTEVAVFVDYESEK

Nucleotide

• Length: 156 bp

>T112522 NZ_CP032808:2901126-2901281 [Escherichia coli]
ATGAAGCAGCAAAAGGCGATGTTAATCGCCCTGATCGTCATCTGTTTAACCGTCATAGTGACGGCACTGGTAACGAGGAA
AGACCTCTGCGAGGTGCGAATCCGAACCGGTCAGACGGAGGTCGCTGTCTTCGTAGACTACGAATCTGAGAAGTAA

Antitoxin

Nucleotide

• Length: 59 bp

>AT112522 NZ_CP032808:c2901114-2901056 [Escherichia coli]
CCTTGCCTTTCGGCACGTAAGAGGCTAACCTACATGTGTTCAGCATGGATTGAGCCTCA

Structures

Toxin

• AlphaFold DB: A0A1Q4PF16

Antitoxin