Detailed information of TA system TA112493

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yafN-yafO (relBE)/YafO-YafN
• Location: 4359379..4360073
• Replicon: chromosome
• Accession: NZ_CP032805
• Organism: Escherichia coli strain ERL05-0623

Toxin (Protein)

• Gene name: yafO
• Uniprot ID: C3TNX7
• Locus tag: D8Z69_RS22470
• Protein ID: WP_001263495.1
• Coordinates: 4359379..4359777 (-)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: yafN
• Uniprot ID: S1FJN6
• Locus tag: D8Z69_RS22475
• Protein ID: WP_000554757.1
• Coordinates: 4359780..4360073 (-)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
-	4354966..4355046	-	81	NuclAT_12	-	-
-	4354966..4355046	-	81	NuclAT_12	-	-
-	4354966..4355046	-	81	NuclAT_12	-	-
-	4354966..4355046	-	81	NuclAT_12	-	-
D8Z69_RS22445	4355642..4356100	-	459	WP_001291990.1	xanthine phosphoribosyltransferase	-
D8Z69_RS22450	4356361..4357818	+	1458	WP_001293009.1	cytosol nonspecific dipeptidase	-
D8Z69_RS22455	4357875..4358396	-	522	Protein_4273	peptide chain release factor H	-
D8Z69_RS22460	4358395..4358598	-	204	Protein_4274	RNA ligase RtcB family protein	-
D8Z69_RS22465	4358917..4359369	-	453	WP_001059871.1	GNAT family N-acetyltransferase	-
D8Z69_RS22470	4359379..4359777	-	399	WP_001263495.1	type II toxin-antitoxin system mRNA interferase toxin YafO	Toxin
D8Z69_RS22475	4359780..4360073	-	294	WP_000554757.1	type I toxin-antitoxin system antitoxin YafN	Antitoxin
D8Z69_RS22480	4360125..4361180	-	1056	WP_001226188.1	DNA polymerase IV	-
D8Z69_RS22485	4361251..4362036	-	786	WP_000207556.1	putative lateral flagellar export/assembly protein LafU	-
D8Z69_RS22490	4362008..4363720	+	1713	Protein_4280	flagellar biosynthesis protein FlhA	-
D8Z69_RS22495	4363865..4364362	-	498	Protein_4281	REP-associated tyrosine transposase RayT	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase toxin YafO (27-130)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 15473.86 Da
• Isoelectric Point: 8.0949

>T112493 WP_001263495.1 NZ_CP032805:c4359777-4359379 [Escherichia coli]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDESHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Nucleotide

• Length: 399 bp

>T112493 NZ_CP032805:c4359777-4359379 [Escherichia coli]
ATGCGGGTATTCAAAACAAAACTTATTCGCCTGCAACTTACAGCAGAGGAGCTTGATGCGTTAACGGCGGATTTTATTTC
CTATAAGCGTGACGGTGTTTTGCCAGATATATTTGGTCGCGATGCACTCTACGACGACTCGTTTACTTGGCCATTAATCA
AATTTGAGCGAGTTGCTCATATTCATCTGGCAAATGTGAATAATCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACG
AATGACGAATCGCATTTGGTATATTGTCAGGGTGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGA
ACCTCATAAACTGGCTCGAGATAACAACCAAATGCATAAAATTGGGAAAATGGCAGAAGCGTTTCGCATGCGTTTTTGA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11233.81 Da
• Isoelectric Point: 5.9050

>AT112493 WP_000554757.1 NZ_CP032805:c4360073-4359780 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EKYLNDMTDDDFNDFKE

Nucleotide

• Length: 294 bp

>AT112493 NZ_CP032805:c4360073-4359780 [Escherichia coli]
ATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAATTACGTAAAAACCCAGCTAAATACTTTATTGATCAACC
GGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGATATCTCTTAAGTGCCAGCGCATTCGAAGCGTTAATGGACATGCTTG
CTGAACAAGAGGAGAAAAAGCCCATAAAGGCGCGCTTCCGTCCAAGTGCTGCAAGATTAGAGGAAATTACACGCCGAGCT
GAAAAATATCTTAATGATATGACGGATGATGATTTCAATGACTTTAAGGAATAA

Structures

Antitoxin

• AlphaFold DB: S1FJN6