Detailed information of TA system TA112424

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yafN-yafO (relBE)/YafO-YafN
• Location: 4473892..4474586
• Replicon: chromosome
• Accession: NZ_CP032801
• Organism: Escherichia coli strain ERL06-2442

Toxin (Protein)

• Gene name: yafO
• Uniprot ID: C3TNX7
• Locus tag: D8Z71_RS23240
• Protein ID: WP_001263495.1
• Coordinates: 4473892..4474290 (-)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: yafN
• Uniprot ID: S1FJN6
• Locus tag: D8Z71_RS23245
• Protein ID: WP_000554757.1
• Coordinates: 4474293..4474586 (-)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
-	4469479..4469559	-	81	NuclAT_12	-	-
-	4469479..4469559	-	81	NuclAT_12	-	-
-	4469479..4469559	-	81	NuclAT_12	-	-
-	4469479..4469559	-	81	NuclAT_12	-	-
D8Z71_RS23215	4470155..4470613	-	459	WP_001291990.1	xanthine phosphoribosyltransferase	-
D8Z71_RS23220	4470874..4472331	+	1458	WP_001293009.1	cytosol nonspecific dipeptidase	-
D8Z71_RS23225	4472388..4472909	-	522	Protein_4407	peptide chain release factor H	-
D8Z71_RS23230	4472908..4473111	-	204	Protein_4408	RNA ligase RtcB family protein	-
D8Z71_RS23235	4473430..4473882	-	453	WP_001059871.1	GNAT family N-acetyltransferase	-
D8Z71_RS23240	4473892..4474290	-	399	WP_001263495.1	type II toxin-antitoxin system mRNA interferase toxin YafO	Toxin
D8Z71_RS23245	4474293..4474586	-	294	WP_000554757.1	type I toxin-antitoxin system antitoxin YafN	Antitoxin
D8Z71_RS23250	4474638..4475693	-	1056	WP_001226186.1	DNA polymerase IV	-
D8Z71_RS23255	4475764..4476549	-	786	WP_000207556.1	putative lateral flagellar export/assembly protein LafU	-
D8Z71_RS23260	4476494..4478233	+	1740	WP_001303130.1	flagellar biosynthesis protein FlhA	-
D8Z71_RS23265	4478378..4478875	-	498	Protein_4415	REP-associated tyrosine transposase RayT	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase toxin YafO (27-130)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 15473.86 Da
• Isoelectric Point: 8.0949

>T112424 WP_001263495.1 NZ_CP032801:c4474290-4473892 [Escherichia coli]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDESHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Nucleotide

• Length: 399 bp

>T112424 NZ_CP032801:c4474290-4473892 [Escherichia coli]
ATGCGGGTATTCAAAACAAAACTTATTCGCCTGCAACTTACAGCAGAGGAGCTTGATGCGTTAACGGCGGATTTTATTTC
CTATAAGCGTGACGGTGTTTTGCCAGATATATTTGGTCGCGATGCACTCTACGACGACTCGTTTACTTGGCCATTAATCA
AATTTGAGCGAGTTGCTCATATTCATCTGGCAAATGTGAATAATCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACG
AATGACGAATCGCATTTGGTATATTGTCAGGGTGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGA
ACCTCATAAACTGGCTCGAGATAACAACCAAATGCATAAAATTGGGAAAATGGCAGAAGCGTTTCGCATGCGTTTTTGA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11233.81 Da
• Isoelectric Point: 5.9050

>AT112424 WP_000554757.1 NZ_CP032801:c4474586-4474293 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EKYLNDMTDDDFNDFKE

Nucleotide

• Length: 294 bp

>AT112424 NZ_CP032801:c4474586-4474293 [Escherichia coli]
ATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAATTACGTAAAAACCCAGCTAAATACTTTATTGATCAACC
GGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGATATCTCTTAAGTGCCAGCGCATTCGAAGCGTTAATGGACATGCTTG
CTGAACAAGAGGAGAAAAAGCCCATAAAGGCGCGCTTCCGTCCAAGTGCTGCAAGATTAGAGGAAATTACACGCCGAGCT
GAAAAATATCTTAATGATATGACGGATGATGATTTCAATGACTTTAAGGAATAA

Structures

Antitoxin

• AlphaFold DB: S1FJN6