Detailed information of TA system TA112393

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 24641..24911
• Replicon: plasmid pERL06-2497-2
• Accession: NZ_CP032799
• Organism: Escherichia coli strain ERL06-2497

Toxin (Protein)

• Gene name: hok
• Uniprot ID: P11895
• Locus tag: D8Z72_RS28835
• Protein ID: WP_001312861.1
• Coordinates: 24753..24911 (+)
• Length: 53 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	24641..24704 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
D8Z72_RS28790	19705..19938	+	234	WP_071588969.1	hypothetical protein	-
D8Z72_RS28810	20411..20950	+	540	WP_009426937.1	single-stranded DNA-binding protein	-
D8Z72_RS28815	21012..21245	+	234	WP_000006030.1	DUF905 family protein	-
D8Z72_RS28820	21310..23268	+	1959	WP_001530506.1	ParB/RepB/Spo0J family partition protein	-
D8Z72_RS28825	23323..23757	+	435	WP_000845923.1	conjugation system SOS inhibitor PsiB	-
D8Z72_RS28830	23754..24473	+	720	WP_001276234.1	plasmid SOS inhibition protein A	-
D8Z72_RS29290	24485..24673	-	189	WP_001336239.1	hypothetical protein	-
-	24485..24709	+	225	NuclAT_0	-	-
-	24485..24709	+	225	NuclAT_0	-	-
-	24485..24709	+	225	NuclAT_0	-	-
-	24485..24709	+	225	NuclAT_0	-	-
-	24641..24704	-	64	-	-	Antitoxin
D8Z72_RS28835	24753..24911	+	159	WP_001312861.1	type I toxin-antitoxin system Hok family toxin	Toxin
D8Z72_RS28850	25832..26119	+	288	WP_000107535.1	hypothetical protein	-
D8Z72_RS28855	26238..27059	+	822	WP_001234469.1	DUF945 domain-containing protein	-
D8Z72_RS28860	27356..27946	-	591	WP_165850686.1	transglycosylase SLT domain-containing protein	-
D8Z72_RS28865	28279..28662	+	384	WP_001151524.1	relaxosome protein TraM	-
D8Z72_RS28870	28849..29538	+	690	WP_000283380.1	conjugal transfer transcriptional regulator TraJ	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	-	1..63089	63089

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (8-48)

Sequences

Toxin

Protein

• Length: 53 a.a.
• Molecular weight: 6082.32 Da
• Isoelectric Point: 9.2584

>T112393 WP_001312861.1 NZ_CP032799:24753-24911 [Escherichia coli]
MKLPRSSLVWCVLIVCLTLLIFTYLTRKSLCEIRYRDGHREVAAFMAYESGK

Nucleotide

• Length: 159 bp

>T112393 NZ_CP032799:24753-24911 [Escherichia coli]
ATGAAACTACCACGAAGTTCCCTTGTCTGGTGTGTGTTGATCGTGTGTCTCACACTGTTGATATTCACTTATCTGACACG
AAAATCGCTGTGCGAGATTCGTTACAGAGACGGACACAGGGAGGTGGCGGCTTTCATGGCTTACGAATCCGGTAAGTAG

Antitoxin

Nucleotide

• Length: 64 bp

>AT112393 NZ_CP032799:c24704-24641 [Escherichia coli]
GACTAGACATAGGGATGCCTCGTGGTGGTTAATGAAAATTAACTTACTACGGGGCTATCTTCTT

Structures

Toxin

• AlphaFold DB: P11895

Antitoxin