Detailed information of TA system TA112311

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 3355325..3355550
• Replicon: chromosome
• Accession: NZ_CP032793
• Organism: Escherichia coli strain NZRM3614

Toxin (Protein)

• Gene name: hokW
• Uniprot ID: -
• Locus tag: D8Z74_RS17390
• Protein ID: WP_000813263.1
• Coordinates: 3355325..3355480 (-)
• Length: 52 a.a.

Antitoxin (RNA)

Gene name	sokW
Locus tag	-
Coordinates	3355492..3355550 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
D8Z74_RS17355	3350779..3351492	-	714	WP_000261909.1	helix-turn-helix domain-containing protein	-
D8Z74_RS17360	3351630..3351826	-	197	Protein_3317	TrmB family transcriptional regulator	-
D8Z74_RS17365	3352113..3352931	-	819	WP_000265265.1	CPBP family intramembrane metalloprotease	-
D8Z74_RS17370	3353083..3353454	-	372	WP_000090264.1	antitermination protein	-
D8Z74_RS17375	3353444..3353815	-	372	WP_001217436.1	RusA family crossover junction endodeoxyribonuclease	-
D8Z74_RS17380	3353828..3354877	-	1050	WP_001265172.1	DUF968 domain-containing protein	-
D8Z74_RS17385	3354879..3355157	-	279	WP_001341388.1	hypothetical protein	-
D8Z74_RS17390	3355325..3355480	-	156	WP_000813263.1	type I toxin-antitoxin system toxin HokD	Toxin
-	3355492..3355550	+	59	-	-	Antitoxin
D8Z74_RS17405	3356085..3356858	+	774	WP_000160654.1	alpha/beta hydrolase	-
D8Z74_RS17410	3357210..3357623	-	414	WP_001151233.1	DUF977 family protein	-
D8Z74_RS17415	3357639..3358409	-	771	WP_000450992.1	DUF1627 domain-containing protein	-
D8Z74_RS17420	3358431..3359177	-	747	WP_000788751.1	ATP-binding protein	-
D8Z74_RS17425	3359184..3360275	-	1092	WP_001205823.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (6-47)

Sequences

Toxin

Protein

• Length: 52 a.a.
• Molecular weight: 5722.86 Da
• Isoelectric Point: 6.1531

>T112311 WP_000813263.1 NZ_CP032793:c3355480-3355325 [Escherichia coli]
MKQQKAMLIALIVICLTVIVTALVTRKDLCEVRVRTGQTEVAVFTAYEPEE

Nucleotide

• Length: 156 bp

>T112311 NZ_CP032793:c3355480-3355325 [Escherichia coli]
ATGAAGCAGCAAAAGGCGATGTTAATCGCCCTGATCGTCATCTGTTTAACCGTCATAGTGACGGCACTGGTAACGAGGAA
AGACCTCTGCGAGGTACGAGTCCGAACCGGCCAGACGGAGGTCGCTGTCTTCACAGCTTACGAACCTGAGGAGTAA

Antitoxin

Nucleotide

• Length: 59 bp

>AT112311 NZ_CP032793:3355492-3355550 [Escherichia coli]
CCTTGCCTTTCGGCACGTAAGAGGCTAACCTAGATTTGCCGTTCATAGATTGAGCCTCA

Structures

Antitoxin