Detailed information of TA system TA112307

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 2525371..2525596
• Replicon: chromosome
• Accession: NZ_CP032793
• Organism: Escherichia coli strain NZRM3614

Toxin (Protein)

• Gene name: hokW
• Uniprot ID: A0A1Q4PF16
• Locus tag: D8Z74_RS12880
• Protein ID: WP_000813258.1
• Coordinates: 2525441..2525596 (+)
• Length: 52 a.a.

Antitoxin (RNA)

Gene name	sokW
Locus tag	-
Coordinates	2525371..2525429 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
D8Z74_RS12830	2520477..2520719	+	243	WP_000747948.1	hypothetical protein	-
D8Z74_RS12835	2520703..2521128	+	426	WP_000693878.1	Rha family transcriptional regulator	-
D8Z74_RS12840	2521197..2522240	+	1044	WP_001262402.1	hypothetical protein	-
D8Z74_RS12845	2522233..2522694	+	462	WP_000139447.1	replication protein	-
D8Z74_RS12850	2522728..2523403	+	676	Protein_2467	DUF1627 domain-containing protein	-
D8Z74_RS12855	2523436..2523717	+	282	WP_000603384.1	DNA-binding protein	-
D8Z74_RS12860	2523714..2523941	+	228	WP_000699809.1	hypothetical protein	-
D8Z74_RS12865	2523934..2524245	+	312	WP_001289673.1	hypothetical protein	-
D8Z74_RS12870	2524373..2524591	+	219	WP_000683609.1	DUF4014 family protein	-
D8Z74_RS12875	2524593..2525150	+	558	WP_000104474.1	DUF551 domain-containing protein	-
-	2525371..2525429	-	59	-	-	Antitoxin
D8Z74_RS12880	2525441..2525596	+	156	WP_000813258.1	type I toxin-antitoxin system Hok family toxin	Toxin
D8Z74_RS12885	2525716..2526060	+	345	WP_000756596.1	YgiW/YdeI family stress tolerance OB fold protein	-
D8Z74_RS12890	2526182..2526454	+	273	WP_000191872.1	hypothetical protein	-
D8Z74_RS12895	2526456..2527505	+	1050	WP_001265229.1	DUF968 domain-containing protein	-
D8Z74_RS12900	2527518..2527823	+	306	WP_001217444.1	RusA family crossover junction endodeoxyribonuclease	-
D8Z74_RS12905	2527886..2528440	+	555	WP_000640035.1	DUF1133 family protein	-
D8Z74_RS12910	2528665..2528862	+	198	WP_000917763.1	hypothetical protein	-
D8Z74_RS12915	2528998..2529711	+	714	WP_000301797.1	helix-turn-helix domain-containing protein	-
D8Z74_RS12930	2530162..2530593	+	432	WP_001302123.1	tellurite resistance protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	nleG7'	2457353..2569610	112257

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (6-47)

Sequences

Toxin

Protein

• Length: 52 a.a.
• Molecular weight: 5767.95 Da
• Isoelectric Point: 7.7942

>T112307 WP_000813258.1 NZ_CP032793:2525441-2525596 [Escherichia coli]
MKQQKAMLIALIVICLTVIVTALVTRKDLCEVRIRTGQTEVAVFVDYESEK

Nucleotide

• Length: 156 bp

>T112307 NZ_CP032793:2525441-2525596 [Escherichia coli]
ATGAAGCAGCAAAAGGCGATGTTAATCGCCCTGATCGTCATCTGTTTAACCGTCATAGTGACGGCACTGGTAACGAGGAA
AGACCTCTGCGAGGTGCGAATCCGAACCGGTCAGACGGAGGTCGCTGTCTTCGTAGACTACGAATCTGAGAAGTAA

Antitoxin

Nucleotide

• Length: 59 bp

>AT112307 NZ_CP032793:c2525429-2525371 [Escherichia coli]
CCTTGCCTTTCGGCACGTAAGAGGCTAACCTACATGTGTTCAGCATGGATTGAGCCTCA

Structures

Toxin

• AlphaFold DB: A0A1Q4PF16

Antitoxin