Detailed information of TA system TA112305

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 2477448..2477673
• Replicon: chromosome
• Accession: NZ_CP032793
• Organism: Escherichia coli strain NZRM3614

Toxin (Protein)

• Gene name: hokW
• Uniprot ID: S1ELB5
• Locus tag: D8Z74_RS12565
• Protein ID: WP_000813254.1
• Coordinates: 2477518..2477673 (+)
• Length: 52 a.a.

Antitoxin (RNA)

Gene name	sokW
Locus tag	-
Coordinates	2477448..2477506 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
D8Z74_RS12520	2472701..2473654	+	954	WP_000095667.1	helix-turn-helix domain-containing protein	-
D8Z74_RS12525	2473661..2474401	+	741	WP_000790456.1	ATP-binding protein	-
D8Z74_RS12530	2474431..2475201	+	771	WP_025840156.1	DUF1627 domain-containing protein	-
D8Z74_RS12535	2475217..2475612	+	396	WP_001118161.1	DUF977 family protein	-
D8Z74_RS12540	2475669..2476025	+	357	WP_001302146.1	hypothetical protein	-
D8Z74_RS12545	2476074..2476286	+	213	WP_000063625.1	hypothetical protein	-
D8Z74_RS12550	2476322..2476693	+	372	WP_000137941.1	hypothetical protein	-
D8Z74_RS12555	2476690..2477052	+	363	WP_000610379.1	DUF551 domain-containing protein	-
D8Z74_RS12560	2477168..2477272	+	105	WP_001278450.1	hypothetical protein	-
-	2477448..2477506	-	59	-	-	Antitoxin
D8Z74_RS12565	2477518..2477673	+	156	WP_000813254.1	type I toxin-antitoxin system toxin HokD	Toxin
D8Z74_RS27850	2477952..2478119	+	168	WP_000998188.1	hypothetical protein	-
D8Z74_RS12570	2478185..2478463	+	279	WP_001304183.1	hypothetical protein	-
D8Z74_RS12575	2478465..2479514	+	1050	WP_001302870.1	DUF968 domain-containing protein	-
D8Z74_RS12580	2479527..2479901	+	375	WP_000904137.1	RusA family crossover junction endodeoxyribonuclease	-
D8Z74_RS12585	2479898..2480719	+	822	WP_000762904.1	antitermination protein	-
D8Z74_RS12590	2480946..2481143	+	198	WP_000917735.1	hypothetical protein	-
D8Z74_RS12595	2481294..2482352	+	1059	WP_000483497.1	site-specific DNA-methyltransferase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	nleG7'	2457353..2569610	112257

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (6-47)

Sequences

Toxin

Protein

• Length: 52 a.a.
• Molecular weight: 5736.89 Da
• Isoelectric Point: 6.1531

>T112305 WP_000813254.1 NZ_CP032793:2477518-2477673 [Escherichia coli]
MKQQKAMLIALIVICLTVIVTALVTRKDLCEVRIRTGQTEVAVFTAYEPEE

Nucleotide

• Length: 156 bp

>T112305 NZ_CP032793:2477518-2477673 [Escherichia coli]
ATGAAGCAGCAAAAGGCGATGCTAATCGCCCTGATCGTCATCTGTTTAACCGTCATAGTGACGGCACTGGTAACGAGGAA
AGACCTCTGCGAGGTACGAATCCGAACCGGCCAGACGGAGGTCGCTGTCTTCACAGCTTACGAACCTGAGGAGTAA

Antitoxin

Nucleotide

• Length: 59 bp

>AT112305 NZ_CP032793:c2477506-2477448 [Escherichia coli]
CCTTGCCTTTCGGCACGTAAGAGGCTAACCTACGTGTGTAGAGCATAGATATGGCCTCA

Structures

Toxin

• AlphaFold DB: A0A4P7TMW0

Antitoxin