Detailed information of TA system    

insolicoBioinformatically predicted

Overview


TA module


Type I Classification (family/domain) symER/SymE(toxin)
Location 4725181..4725593 Replicon chromosome
Accession NZ_CP032791
Organism Escherichia coli strain NZRM4165

Toxin (Protein)


Gene name symE Uniprot ID Q8FA88
Locus tag D8Z75_RS24145 Protein ID WP_000132630.1
Coordinates 4725252..4725593 (+) Length 114 a.a.

Antitoxin (RNA)


Gene name symR
Locus tag -
Coordinates 4725181..4725257 (-)

Genomic Context


Locus tag Coordinates Strand Size (bp) Protein ID Product Description
D8Z75_RS24135 4721808..4723277 + 1470 WP_001302468.1 type I restriction-modification system subunit M -
D8Z75_RS24140 4723277..4725031 + 1755 WP_000110082.1 restriction endonuclease subunit S -
- 4725181..4725257 - 77 NuclAT_14 - Antitoxin
- 4725181..4725257 - 77 NuclAT_14 - Antitoxin
- 4725181..4725257 - 77 NuclAT_14 - Antitoxin
- 4725181..4725257 - 77 NuclAT_14 - Antitoxin
- 4725181..4725257 - 77 NuclAT_15 - Antitoxin
- 4725181..4725257 - 77 NuclAT_15 - Antitoxin
- 4725181..4725257 - 77 NuclAT_15 - Antitoxin
- 4725181..4725257 - 77 NuclAT_15 - Antitoxin
D8Z75_RS24145 4725252..4725593 + 342 WP_000132630.1 endoribonuclease SymE Toxin
D8Z75_RS24150 4725640..4726803 - 1164 WP_001304006.1 DUF1524 domain-containing protein -
D8Z75_RS24155 4726851..4727732 - 882 WP_001304007.1 DUF262 domain-containing protein -
D8Z75_RS24160 4727829..4727993 - 165 WP_000394274.1 DUF1127 domain-containing protein -
D8Z75_RS24165 4728170..4729582 + 1413 WP_000199295.1 PLP-dependent aminotransferase family protein -

Associated MGEs


MGE
detail
Similar
MGEs
Relative
position
MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Genomic island - espX6 4717380..4738606 21226


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains


Predicted by InterproScan

Toxin

(20-71)


Sequences


Toxin        


Download         Length: 114 a.a.        Molecular weight: 12294.13 Da        Isoelectric Point: 8.4982

>T112282 WP_000132630.1 NZ_CP032791:4725252-4725593 [Escherichia coli]
MTDTHSIAQPFEAEVSPANNRQLTVSYASRYPDYSRIPAITLKGQWLEAAGFATGTVVDVKVMEGCIVLTAQPPAAAESE
LMQSLRQVCKLSARKQRQVQEFIGVIAGKQKVA

Download         Length: 342 bp

>T112282 NZ_CP032791:4725252-4725593 [Escherichia coli]
ATGACTGATACGCATTCTATTGCACAACCGTTCGAAGCAGAAGTCTCCCCGGCAAATAACCGTCAATTAACCGTGAGTTA
TGCGAGTCGTTACCCGGATTACAGCCGTATTCCCGCCATCACCCTGAAAGGTCAGTGGCTGGAAGCCGCCGGTTTTGCCA
CCGGCACGGTGGTAGATGTCAAAGTGATGGAAGGCTGTATTGTCCTCACCGCCCAACCACCCGCCGCCGCCGAGAGCGAA
CTGATGCAGTCGCTGCGCCAGGTGTGCAAGCTGTCGGCGCGTAAACAAAGGCAGGTGCAGGAGTTTATTGGGGTGATTGC
GGGTAAACAGAAAGTTGCCTGA

Antitoxin


Download         Length: 77 bp

>AT112282 NZ_CP032791:c4725257-4725181 [Escherichia coli]
AGTCATAACTGCTATTCCCTATAAATAGTGATTGTGATTAGCGGTGCGGGTGTGTTGGCGCACATCCGCACCGCGCT

Similar Proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures


Toxin

Source ID Structure
AlphaFold DB A0A0E0Y5T0


Antitoxin

Download structure file

References