Detailed information of TA system TA112271

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 2664647..2664872
• Replicon: chromosome
• Accession: NZ_CP032791
• Organism: Escherichia coli strain NZRM4165

Toxin (Protein)

• Gene name: hokW
• Uniprot ID: -
• Locus tag: D8Z75_RS13480
• Protein ID: WP_000935259.1
• Coordinates: 2664647..2664859 (-)
• Length: 71 a.a.

Antitoxin (RNA)

Gene name	sokW
Locus tag	-
Coordinates	2664814..2664872 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
D8Z75_RS13430	2659650..2660081	-	432	WP_001302123.1	tellurite resistance protein	-
D8Z75_RS13445	2660532..2661245	-	714	WP_000301797.1	helix-turn-helix domain-containing protein	-
D8Z75_RS13450	2661381..2661578	-	198	WP_000917763.1	hypothetical protein	-
D8Z75_RS13455	2661803..2662357	-	555	WP_000640035.1	DUF1133 family protein	-
D8Z75_RS13460	2662420..2662725	-	306	WP_001217444.1	RusA family crossover junction endodeoxyribonuclease	-
D8Z75_RS13465	2662738..2663787	-	1050	WP_001265229.1	DUF968 domain-containing protein	-
D8Z75_RS13470	2663789..2664061	-	273	WP_000191872.1	hypothetical protein	-
D8Z75_RS13475	2664183..2664527	-	345	WP_000756596.1	YgiW/YdeI family stress tolerance OB fold protein	-
D8Z75_RS13480	2664647..2664859	-	213	WP_000935259.1	type I toxin-antitoxin system Hok family toxin	Toxin
-	2664814..2664872	+	59	-	-	Antitoxin
D8Z75_RS13485	2665093..2665650	-	558	WP_000104474.1	DUF551 domain-containing protein	-
D8Z75_RS13490	2665652..2665870	-	219	WP_000683609.1	DUF4014 family protein	-
D8Z75_RS13495	2665998..2666309	-	312	WP_001289673.1	hypothetical protein	-
D8Z75_RS13500	2666302..2666529	-	228	WP_000699809.1	hypothetical protein	-
D8Z75_RS13505	2666526..2666807	-	282	WP_000603384.1	DNA-binding protein	-
D8Z75_RS13510	2666840..2667556	-	717	WP_000450627.1	DUF1627 domain-containing protein	-
D8Z75_RS13515	2667590..2668051	-	462	WP_000139447.1	replication protein	-
D8Z75_RS13520	2668044..2669087	-	1044	WP_001262402.1	hypothetical protein	-
D8Z75_RS13525	2669156..2669581	-	426	WP_000693878.1	Rha family transcriptional regulator	-
D8Z75_RS13530	2669565..2669807	-	243	WP_000747948.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	espJ / espFu/tccP / nleG7' / paa	2625365..2725529	100164

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (25-66)

Sequences

Toxin

Protein

• Length: 71 a.a.
• Molecular weight: 7887.47 Da
• Isoelectric Point: 9.2654

>T112271 WP_000935259.1 NZ_CP032791:c2664859-2664647 [Escherichia coli]
MLNTCRLASYVPKGKEKQAMKQQKAMLIALIVICLTVIVTALVTRKDLCEVRIRTGQTEVAVFVDYESEK

Nucleotide

• Length: 213 bp

>T112271 NZ_CP032791:c2664859-2664647 [Escherichia coli]
ATGCTGAACACATGTAGGTTAGCCTCTTACGTGCCGAAAGGCAAGGAGAAGCAGGCTATGAAGCAGCAAAAGGCGATGTT
AATCGCCCTGATCGTCATCTGTTTAACCGTCATAGTGACGGCACTGGTAACGAGGAAAGACCTCTGCGAGGTGCGAATCC
GAACCGGTCAGACGGAGGTCGCTGTCTTCGTAGACTACGAATCTGAGAAGTAA

Antitoxin

Nucleotide

• Length: 59 bp

>AT112271 NZ_CP032791:2664814-2664872 [Escherichia coli]
CCTTGCCTTTCGGCACGTAAGAGGCTAACCTACATGTGTTCAGCATGGATTGAGCCTCA

Structures

Antitoxin