Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) DinJ-YafQ (relBE)/YafQ-RelB
Location 4434073..4434612 Replicon chromosome
Accession NZ_CP032789
Organism Escherichia coli strain NZRM4169

Toxin (Protein)


Gene name yafQ Uniprot ID -
Locus tag D8Z76_RS23060 Protein ID WP_001303998.1
Coordinates 4434352..4434612 (+) Length 87 a.a.

Antitoxin (Protein)


Gene name dinJ Uniprot ID C3TP07
Locus tag D8Z76_RS23055 Protein ID WP_000729705.1
Coordinates 4434073..4434333 (+) Length 87 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
D8Z76_RS23035 4429827..4430612 - 786 WP_000207556.1 putative lateral flagellar export/assembly protein LafU -
D8Z76_RS23040 4430584..4432296 + 1713 Protein_4383 flagellar biosynthesis protein FlhA -
D8Z76_RS23045 4432441..4432938 - 498 Protein_4384 REP-associated tyrosine transposase RayT -
D8Z76_RS23050 4433114..4433887 - 774 WP_000543891.1 C40 family peptidase -
D8Z76_RS23055 4434073..4434333 + 261 WP_000729705.1 type II toxin-antitoxin system antitoxin DinJ Antitoxin
D8Z76_RS23060 4434352..4434612 + 261 WP_001303998.1 type II toxin-antitoxin system mRNA interferase toxin YafQ Toxin
D8Z76_RS23065 4434768..4435508 + 741 WP_001225679.1 murein L,D-transpeptidase -
D8Z76_RS23070 4435479..4436246 - 768 WP_001301698.1 class II glutamine amidotransferase -
D8Z76_RS23075 4436351..4436929 - 579 WP_000284050.1 D-sedoheptulose 7-phosphate isomerase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, YafQ-like toxin

(3-85)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(1-85)


Sequences

Toxin        


Download         Length: 87 a.a.        Molecular weight: 10026.71 Da        Isoelectric Point: 10.3188

>T112244 WP_001303998.1 NZ_CP032789:4434352-4434612 [Escherichia coli]
MNSGQFSKDVKLAQKRHKDMNKLKYLMTLLINNTLPLPAVYKDHPLQGSWKGYRDAHVEPDWILIYKLTDKLLRFERTGT
HAALFG

Download         Length: 261 bp

>T112244 NZ_CP032789:4434352-4434612 [Escherichia coli]
TTGAACTCGGGACAATTTTCAAAGGATGTAAAACTTGCACAAAAGCGTCATAAGGATATGAATAAATTGAAATATCTTAT
GACGCTTCTTATCAATAATACTTTACCGCTTCCAGCTGTTTATAAAGACCACCCGCTGCAAGGTTCATGGAAAGGTTATC
GCGATGCTCATGTCGAACCGGACTGGATCCTGATTTACAAACTTACCGATAAACTTTTACGATTTGAGAGAACTGGAACT
CACGCGGCGCTCTTTGGGTAA

Antitoxin


Download         Length: 87 a.a.        Molecular weight: 9377.74 Da        Isoelectric Point: 5.0758

>AT112244 WP_000729705.1 NZ_CP032789:4434073-4434333 [Escherichia coli]
MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVAREKALPFDLREPNQLTIQSIKNSEAGVDVHKAKDADDL
FDKLGV

Download         Length: 261 bp

>AT112244 NZ_CP032789:4434073-4434333 [Escherichia coli]
ATGGCTGCTAACGCATTTGTTCGCGCCCGAATCGATGAAGATCTGAAGAATCAGGCGGCGGACGTACTGGCCGGGATGGG
GCTGACCATCTCTGACCTGGTTCGCATAACCCTCACAAAGGTCGCGCGTGAAAAGGCATTGCCGTTTGATTTACGCGAGC
CTAATCAATTAACCATTCAATCAATCAAAAACAGCGAAGCTGGCGTTGATGTTCATAAGGCCAAAGACGCCGATGATTTA
TTTGATAAATTAGGAGTTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure

References