Detailed information of TA system TA112243

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yafN-yafO (relBE)/YafO-YafN
• Location: 4427955..4428649
• Replicon: chromosome
• Accession: NZ_CP032789
• Organism: Escherichia coli strain NZRM4169

Toxin (Protein)

• Gene name: yafO
• Uniprot ID: C3TNX7
• Locus tag: D8Z76_RS23020
• Protein ID: WP_001263495.1
• Coordinates: 4427955..4428353 (-)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: yafN
• Uniprot ID: S1FJN6
• Locus tag: D8Z76_RS23025
• Protein ID: WP_000554757.1
• Coordinates: 4428356..4428649 (-)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
-	4423542..4423622	-	81	NuclAT_12	-	-
-	4423542..4423622	-	81	NuclAT_12	-	-
-	4423542..4423622	-	81	NuclAT_12	-	-
-	4423542..4423622	-	81	NuclAT_12	-	-
D8Z76_RS22995	4424218..4424676	-	459	WP_001291990.1	xanthine phosphoribosyltransferase	-
D8Z76_RS23000	4424937..4426394	+	1458	WP_001293009.1	cytosol nonspecific dipeptidase	-
D8Z76_RS23005	4426451..4426972	-	522	Protein_4376	peptide chain release factor H	-
D8Z76_RS23010	4426971..4427174	-	204	Protein_4377	RNA ligase RtcB family protein	-
D8Z76_RS23015	4427493..4427945	-	453	WP_001059871.1	GNAT family N-acetyltransferase	-
D8Z76_RS23020	4427955..4428353	-	399	WP_001263495.1	type II toxin-antitoxin system mRNA interferase toxin YafO	Toxin
D8Z76_RS23025	4428356..4428649	-	294	WP_000554757.1	type I toxin-antitoxin system antitoxin YafN	Antitoxin
D8Z76_RS23030	4428701..4429756	-	1056	WP_001226186.1	DNA polymerase IV	-
D8Z76_RS23035	4429827..4430612	-	786	WP_000207556.1	putative lateral flagellar export/assembly protein LafU	-
D8Z76_RS23040	4430584..4432296	+	1713	Protein_4383	flagellar biosynthesis protein FlhA	-
D8Z76_RS23045	4432441..4432938	-	498	Protein_4384	REP-associated tyrosine transposase RayT	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase toxin YafO (27-130)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 15473.86 Da
• Isoelectric Point: 8.0949

>T112243 WP_001263495.1 NZ_CP032789:c4428353-4427955 [Escherichia coli]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDESHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Nucleotide

• Length: 399 bp

>T112243 NZ_CP032789:c4428353-4427955 [Escherichia coli]
ATGCGGGTATTCAAAACAAAACTTATTCGCCTGCAACTTACAGCAGAGGAGCTTGATGCGTTAACGGCGGATTTTATTTC
CTATAAGCGTGACGGTGTTTTGCCAGATATATTTGGTCGCGATGCACTCTACGACGACTCGTTTACTTGGCCATTAATCA
AATTTGAGCGAGTTGCTCATATTCATCTGGCAAATGTGAATAATCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACG
AATGACGAATCGCATTTGGTATATTGTCAGGGTGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGA
ACCTCATAAACTGGCTCGAGATAACAACCAAATGCATAAAATTGGGAAAATGGCAGAAGCGTTTCGCATGCGTTTTTGA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11233.81 Da
• Isoelectric Point: 5.9050

>AT112243 WP_000554757.1 NZ_CP032789:c4428649-4428356 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EKYLNDMTDDDFNDFKE

Nucleotide

• Length: 294 bp

>AT112243 NZ_CP032789:c4428649-4428356 [Escherichia coli]
ATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAATTACGTAAAAACCCAGCTAAATACTTTATTGATCAACC
GGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGATATCTCTTAAGTGCCAGCGCATTCGAAGCGTTAATGGACATGCTTG
CTGAACAAGAGGAGAAAAAGCCCATAAAGGCGCGCTTCCGTCCAAGTGCTGCAAGATTAGAGGAAATTACACGCCGAGCT
GAAAAATATCTTAATGATATGACGGATGATGATTTCAATGACTTTAAGGAATAA

Structures

Antitoxin

• AlphaFold DB: S1FJN6