Detailed information of TA system TA112226

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): ralRA/-
• Location: 2244598..2244969
• Replicon: chromosome
• Accession: NZ_CP032789
• Organism: Escherichia coli strain NZRM4169

Toxin (Protein)

• Gene name: ralR
• Uniprot ID: Q8Z5Y6
• Locus tag: D8Z76_RS11515
• Protein ID: WP_010989194.1
• Coordinates: 2244775..2244969 (-)
• Length: 65 a.a.

Antitoxin (RNA)

Gene name	ralA
Locus tag	-
Coordinates	2244598..2244776 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
D8Z76_RS11475	2240040..2240696	-	657	WP_000011656.1	carbon-nitrogen hydrolase family protein	-
D8Z76_RS11480	2240798..2241028	-	231	WP_000916763.1	DNA polymerase III subunit theta	-
D8Z76_RS11485	2241167..2241541	+	375	WP_000168751.1	CopC domain-containing protein YobA	-
D8Z76_RS11490	2241545..2242417	+	873	WP_000879314.1	copper homeostasis membrane protein CopD	-
D8Z76_RS11495	2242430..2242771	+	342	WP_000976483.1	YebY family protein	-
D8Z76_RS11500	2243164..2244240	-	1077	WP_001189085.1	phage integrase Arm DNA-binding domain-containing protein	-
D8Z76_RS11505	2244206..2244487	-	282	WP_005127484.1	excisionase	-
D8Z76_RS11510	2244594..2244782	-	189	WP_151538908.1	DUF1187 family protein	-
-	2244598..2244776	+	179	NuclAT_0	-	Antitoxin
-	2244598..2244776	+	179	NuclAT_0	-	Antitoxin
-	2244598..2244776	+	179	NuclAT_0	-	Antitoxin
-	2244598..2244776	+	179	NuclAT_0	-	Antitoxin
D8Z76_RS11515	2244775..2244969	-	195	WP_010989194.1	type I toxin-antitoxin system endodeoxyribonuclease toxin RalR	Toxin
D8Z76_RS11520	2245033..2246085	-	1053	WP_001004413.1	recombinase RecT	-
D8Z76_RS11525	2246097..2249246	-	3150	WP_069192725.1	PD-(D/E)XK nuclease-like domain-containing protein	-
D8Z76_RS11530	2249347..2249622	-	276	WP_151538909.1	hypothetical protein	-
D8Z76_RS11535	2249697..2249867	-	171	WP_000358365.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	nleC	2237297..2313049	75752

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Endodeoxyribonuclease toxin RalR (6-54)

Sequences

Toxin

Protein

• Length: 65 a.a.
• Molecular weight: 7077.01 Da
• Isoelectric Point: 9.2886

>T112226 WP_010989194.1 NZ_CP032789:c2244969-2244775 [Escherichia coli]
MRYEKVKPCPFCGCPSVTVKAISGYYRAKCNGCESRTGYSGSEKEALERWNKRTTGNNNGGVHV

Nucleotide

• Length: 195 bp

>T112226 NZ_CP032789:c2244969-2244775 [Escherichia coli]
ATGCGGTATGAAAAAGTCAAACCATGTCCGTTTTGTGGTTGTCCATCAGTAACGGTGAAAGCCATTTCAGGATATTACCG
CGCAAAGTGTAACGGATGCGAATCCCGAACCGGCTATAGTGGAAGTGAAAAAGAAGCACTCGAAAGATGGAATAAACGAA
CTACTGGAAATAATAATGGAGGTGTTCATGTATAA

Antitoxin

Nucleotide

• Length: 179 bp

>AT112226 NZ_CP032789:2244598-2244776 [Escherichia coli]
GAGGGCAGAAGTTTCTCGCAATTAAAATTTATCAGCTTTACTTTCTGCTCTCTGGACACGCTTGCTTCTTTTTTCCCAGA
GAGCATTTTTTCGCATTCTGATTTCGTTAATTTAGATTTTGAATATCTTGTCCAGTTAGTAGGAGTGCCACCTTCCTTTT
CAATAGTGGCAGTAATTTT

Structures

Toxin

• AlphaFold DB: A0A716R3V7

Antitoxin