Detailed information of TA system TA112208

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 5977934..5978529
• Replicon: chromosome
• Accession: NZ_CP032761
• Organism: Pseudomonas aeruginosa strain 268

Toxin (Protein)

• Gene name: higB
• Uniprot ID: V6ALY3
• Locus tag: PA268_RS28960
• Protein ID: WP_003113526.1
• Coordinates: 5978251..5978529 (-)
• Length: 93 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: PA268_RS28955
• Protein ID: WP_003133769.1
• Coordinates: 5977934..5978239 (-)
• Length: 102 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
PA268_RS28920	5973085..5973933	+	849	WP_003095007.1	4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol kinase	-
PA268_RS28930	5974100..5975041	+	942	WP_003099281.1	ribose-phosphate pyrophosphokinase	-
PA268_RS28935	5975158..5975772	+	615	WP_003095013.1	50S ribosomal protein L25/general stress protein Ctc	-
PA268_RS28940	5975814..5976398	+	585	WP_003099278.1	aminoacyl-tRNA hydrolase	-
PA268_RS28945	5976439..5977539	+	1101	WP_003095017.1	redox-regulated ATPase YchF	-
PA268_RS28955	5977934..5978239	-	306	WP_003133769.1	HigA family addiction module antidote protein	Antitoxin
PA268_RS28960	5978251..5978529	-	279	WP_003113526.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
PA268_RS28970	5978858..5981086	+	2229	WP_023104079.1	TonB-dependent receptor	-
PA268_RS28975	5981157..5981804	-	648	WP_003095021.1	carbonate dehydratase	-
PA268_RS28980	5981866..5983104	-	1239	WP_003095023.1	C69 family dipeptidase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (5-92)

Antitoxin

• Toxin-antitoxin system, antidote protein, HigA (6-82)

Sequences

Toxin

Protein

• Length: 93 a.a.
• Molecular weight: 10646.19 Da
• Isoelectric Point: 7.8937

>T112208 WP_003113526.1 NZ_CP032761:c5978529-5978251 [Pseudomonas aeruginosa]
MILTFRCDETRQLFETGLSRRWGAILTVATRKLAMLHAATELRDLRSPPGNRLEPLQGKRAGQHSIRINDQWRVCFVWTD
AGPEEVEIVDYH

Nucleotide

• Length: 279 bp

>T112208 NZ_CP032761:c5978529-5978251 [Pseudomonas aeruginosa]
ATGATTCTGACCTTTCGCTGCGACGAGACTCGTCAGCTTTTTGAGACGGGTCTTTCGAGGCGGTGGGGAGCGATCCTCAC
AGTCGCTACGCGTAAGCTCGCAATGCTTCATGCGGCTACGGAGCTTCGAGACTTGCGCTCTCCACCTGGAAACCGGTTGG
AGCCGTTGCAGGGAAAGCGGGCGGGCCAACATAGCATCAGGATCAATGACCAGTGGCGTGTCTGTTTCGTCTGGACGGAT
GCGGGTCCCGAAGAAGTCGAAATAGTTGATTACCACTGA

Antitoxin

Protein

• Length: 102 a.a.
• Molecular weight: 11214.73 Da
• Isoelectric Point: 4.6081

>AT112208 WP_003133769.1 NZ_CP032761:c5978239-5977934 [Pseudomonas aeruginosa]
MATNGMRPIHPGEILRDEFLMELDISPAALARALKVSAPTVNDIVREQRGISADMAIRLGRYFDTSAQFWMNLQSEYSLA
TAYAANGEQIEHEIEPLLAHG

Nucleotide

• Length: 306 bp

>AT112208 NZ_CP032761:c5978239-5977934 [Pseudomonas aeruginosa]
ATGGCTACCAATGGTATGCGCCCCATCCATCCTGGGGAAATATTGCGCGATGAGTTTCTGATGGAGTTGGATATCTCTCC
AGCTGCTCTAGCACGCGCTTTGAAAGTCTCCGCTCCGACAGTGAATGATATCGTTCGTGAGCAGCGTGGTATCTCCGCAG
ATATGGCGATTCGTCTGGGGCGTTACTTTGATACGTCCGCTCAGTTCTGGATGAATCTCCAGAGTGAGTATTCGTTAGCA
ACTGCTTATGCGGCGAATGGCGAGCAGATCGAACATGAGATTGAGCCGCTGCTTGCTCACGGATAG

Structures

Toxin

• AlphaFold DB: V6ALY3