Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/COG4683-HTH_37
Location 229213..229868 Replicon plasmid unnamed2
Accession NZ_CP032704
Organism Pantoea dispersa strain DSM 32899

Toxin (Protein)


Gene name higB Uniprot ID A0A2S5K5W2
Locus tag D8B20_RS21065 Protein ID WP_065647947.1
Coordinates 229213..229572 (+) Length 120 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID A0A2S5K5S9
Locus tag D8B20_RS21070 Protein ID WP_065647948.1
Coordinates 229569..229868 (+) Length 100 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
D8B20_RS21045 225757..226575 + 819 WP_145891983.1 AraC family transcriptional regulator -
D8B20_RS21050 226546..227184 - 639 WP_065647944.1 LysE family transporter -
D8B20_RS21055 227181..228482 - 1302 WP_145891985.1 hypothetical protein -
D8B20_RS21060 228710..228982 + 273 WP_104189439.1 antitoxin -
D8B20_RS21065 229213..229572 + 360 WP_065647947.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
D8B20_RS21070 229569..229868 + 300 WP_065647948.1 XRE family transcriptional regulator Antitoxin
D8B20_RS21075 230057..231130 - 1074 Protein_206 translesion error-prone DNA polymerase V subunit UmuC -
D8B20_RS21080 231169..232152 + 984 WP_141496964.1 tyrosine-type recombinase/integrase -
D8B20_RS21085 232154..234271 + 2118 WP_141496963.1 tyrosine-type recombinase/integrase -
D8B20_RS21090 234396..234797 + 402 WP_145891987.1 VOC family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - - 1..275665 275665


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-like

(12-107)

Antitoxin

HigA2-like, helix-turn-helix domain

(8-87)


Sequences

Toxin        


Download         Length: 120 a.a.        Molecular weight: 13808.93 Da        Isoelectric Point: 9.8337

>T112180 WP_065647947.1 NZ_CP032704:229213-229572 [Pantoea dispersa]
VWIVETTDLFAEWLREQDRAMQLDVLAALNLLKQEGPHLGRPHVDTLYGSKIPNMKELRVQSNGRPVRGFFVFDPMRKAI
VLCAGNKEGADEKRFYKTMIKTAENEYHRHLDQLKRSGK

Download         Length: 360 bp

>T112180 NZ_CP032704:229213-229572 [Pantoea dispersa]
GTGTGGATCGTTGAAACCACGGATTTATTCGCGGAATGGCTCAGAGAGCAGGACAGGGCGATGCAGCTGGATGTGCTTGC
CGCACTGAACCTTCTGAAGCAGGAAGGTCCGCATCTTGGCCGGCCGCATGTGGATACGCTTTACGGCTCAAAAATCCCGA
ACATGAAAGAACTGAGGGTACAGAGCAACGGGCGGCCCGTAAGAGGGTTCTTTGTGTTTGACCCCATGCGTAAAGCGATT
GTCCTGTGTGCGGGTAATAAAGAAGGCGCAGATGAAAAGCGCTTCTACAAGACCATGATTAAAACAGCGGAAAATGAGTA
CCACCGACATCTGGACCAACTGAAACGGAGCGGGAAATGA

Antitoxin


Download         Length: 100 a.a.        Molecular weight: 10810.43 Da        Isoelectric Point: 4.8307

>AT112180 WP_065647948.1 NZ_CP032704:229569-229868 [Pantoea dispersa]
MKTLDDLMADLTPDELTQVHEKVAAMRLDSQLYKLREALSKTQKQQAEAMGIAQPSVAAIEGRGEDLKIATLKRYVEALG
GQLTIGVNMPGGEHVDFTL

Download         Length: 300 bp

>AT112180 NZ_CP032704:229569-229868 [Pantoea dispersa]
ATGAAAACGCTTGATGACCTGATGGCTGATCTGACACCTGATGAACTCACTCAGGTTCATGAAAAAGTCGCGGCAATGCG
ACTTGATTCTCAGCTGTATAAACTGCGCGAGGCGCTGTCGAAAACCCAAAAGCAGCAGGCTGAAGCCATGGGTATTGCAC
AGCCTTCTGTTGCGGCTATTGAAGGGCGCGGTGAAGATCTCAAAATTGCCACGCTCAAACGTTATGTTGAGGCGCTTGGC
GGTCAGCTGACAATTGGCGTCAATATGCCTGGCGGTGAGCACGTTGATTTTACCCTCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2S5K5W2

Antitoxin

Source ID Structure
AlphaFold DB A0A2S5K5S9

References