Detailed information of TA system TA112122

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mqsRA (relBE)/MqsR-MqsA
• Location: 3167854..3168547
• Replicon: chromosome
• Accession: NZ_CP032679
• Organism: Escherichia coli str. K-12 substr. MG1655 strain K-12

Toxin (Protein)

• Gene name: mqsR
• Uniprot ID: S1EZG2
• Locus tag: D7U33_RS15350
• Protein ID: WP_000415584.1
• Coordinates: 3168251..3168547 (-)
• Length: 99 a.a.

Antitoxin (Protein)

• Gene name: mqsA
• Uniprot ID: S1EBV2
• Locus tag: D7U33_RS15345
• Protein ID: WP_000650107.1
• Coordinates: 3167854..3168249 (-)
• Length: 132 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
D7U33_RS15335	3163718..3165976	-	2259	WP_001281881.1	DNA topoisomerase IV subunit A	-
D7U33_RS15340	3166114..3167721	-	1608	WP_001295629.1	ABC transporter substrate-binding protein	-
D7U33_RS15345	3167854..3168249	-	396	WP_000650107.1	type II toxin-antitoxin system antitoxin MqsA	Antitoxin
D7U33_RS15350	3168251..3168547	-	297	WP_000415584.1	type II toxin-antitoxin system toxin MqsR	Toxin
D7U33_RS15355	3168752..3169234	-	483	WP_000183505.1	transcriptional regulator	-
D7U33_RS15360	3169287..3169679	-	393	WP_000712658.1	OB fold stress tolerance protein YgiW	-
D7U33_RS15365	3169831..3170490	+	660	WP_001221495.1	two-component system response regulator QseB	-
D7U33_RS15370	3170487..3171836	+	1350	WP_000673402.1	two-component system sensor histidine kinase QseC	-
D7U33_RS15375	3171882..3172214	-	333	WP_000917684.1	DUF2645 family protein	-
D7U33_RS15380	3172533..3173114	+	582	WP_000065430.1	NADPH:quinone oxidoreductase MdaB	-
D7U33_RS15385	3173145..3173459	+	315	WP_000958598.1	antibiotic biosynthesis monooxygenase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase toxin MqsR (5-97)

Antitoxin

• Zinc finger, MqsA-type (3-47)

Sequences

Toxin

Protein

• Length: 99 a.a.
• Molecular weight: 11231.96 Da
• Isoelectric Point: 8.9070

>T112122 WP_000415584.1 NZ_CP032679:c3168547-3168251 [Escherichia coli str. K-12 substr. MG1655]
MEKRTPHTRLSQVKKLVNAGQVRTTRSALLNADELGLDFDGMCNVIIGLSESDFYKSMTTYSDHTIWQDVYRPRLVTGQV
YLKITVIHDVLIVSFKEK

Nucleotide

• Length: 297 bp

>T112122 NZ_CP032679:c3168547-3168251 [Escherichia coli str. K-12 substr. MG1655]
ATGGAAAAACGCACACCACATACACGTTTGAGTCAGGTTAAAAAACTTGTCAATGCCGGGCAAGTTCGTACAACACGTAG
TGCCCTGTTAAATGCAGATGAGTTAGGTTTGGATTTTGATGGTATGTGTAATGTTATCATTGGATTATCAGAGAGCGACT
TTTATAAAAGCATGACCACCTACTCTGATCATACTATCTGGCAGGATGTTTACAGACCCAGGCTTGTTACAGGCCAGGTT
TATCTTAAAATTACGGTAATTCATGACGTACTGATCGTCTCGTTTAAGGAGAAGTAA

Antitoxin

Protein

• Length: 132 a.a.
• Molecular weight: 14703.10 Da
• Isoelectric Point: 9.2136

>AT112122 WP_000650107.1 NZ_CP032679:c3168249-3167854 [Escherichia coli str. K-12 substr. MG1655]
MKCPVCHQGEMVSGIKDIPYTFRGRKTVLKGIHGLYCVHCEESIMNKEESDAFMAQVKAFRASVNAETVAPEFIVKVRKK
LSLTQKEASEIFGGGVNAFSRYEKGNAQPHPSTIKLLRVLDKHPELLNEIR

Nucleotide

• Length: 396 bp

>AT112122 NZ_CP032679:c3168249-3167854 [Escherichia coli str. K-12 substr. MG1655]
ATGAAATGTCCGGTTTGCCACCAGGGAGAAATGGTTTCTGGCATTAAAGATATTCCATACACCTTCCGTGGACGAAAAAC
AGTATTGAAAGGTATCCACGGTTTATATTGTGTCCATTGCGAAGAGAGCATCATGAATAAAGAAGAGTCAGATGCTTTCA
TGGCGCAAGTAAAGGCATTTCGGGCTTCGGTGAATGCCGAAACAGTGGCACCTGAATTTATAGTGAAGGTTCGAAAAAAG
CTCTCTCTTACCCAAAAAGAGGCAAGCGAAATTTTTGGGGGAGGTGTAAATGCGTTTTCGCGTTACGAAAAAGGCAATGC
CCAACCTCATCCTTCCACAATCAAACTTTTACGTGTTCTGGATAAGCATCCAGAACTATTGAATGAAATCCGTTAA

Structures

Toxin

• PDB: 3HI2

Antitoxin

• PDB: 3HI2
• PDB: 3GA8
• PDB: 3O9X
• PDB: 2KZ8
• PDB: 3FMY
• PDB: 3GN5
• AlphaFold DB: A0A7U9LU65