Detailed information of TA system TA112092

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yafN-yafO (relBE)/YafO-YafN
• Location: 252004..252698
• Replicon: chromosome
• Accession: NZ_CP032679
• Organism: Escherichia coli str. K-12 substr. MG1655 strain K-12

Toxin (Protein)

• Gene name: yafO
• Uniprot ID: Q47157
• Locus tag: D7U33_RS01180
• Protein ID: WP_001263489.1
• Coordinates: 252300..252698 (+)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: yafN
• Uniprot ID: S1QAE3
• Locus tag: D7U33_RS01175
• Protein ID: WP_000554758.1
• Coordinates: 252004..252297 (+)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
D7U33_RS01155	247636..248133	+	498	WP_000006255.1	REP-associated tyrosine transposase RayT	-
D7U33_RS01160	248357..250096	-	1740	WP_001334802.1	flagellar biosynthesis protein FlhA	-
D7U33_RS01165	250041..250826	+	786	WP_000207552.1	putative lateral flagellar export/assembly protein LafU	-
D7U33_RS01170	250897..251952	+	1056	WP_001226164.1	DNA polymerase IV	-
D7U33_RS01175	252004..252297	+	294	WP_000554758.1	type I toxin-antitoxin system antitoxin YafN	Antitoxin
D7U33_RS01180	252300..252698	+	399	WP_001263489.1	type II toxin-antitoxin system mRNA interferase toxin YafO	Toxin
D7U33_RS01185	252708..253160	+	453	WP_001059892.1	GNAT family N-acetyltransferase	-
D7U33_RS01190	253478..253684	+	207	Protein_230	RtcB family protein	-
D7U33_RS01195	253680..254201	+	522	Protein_231	peptide chain release factor H	-
D7U33_RS01200	254258..255715	-	1458	WP_001292994.1	cytosol nonspecific dipeptidase	-
D7U33_RS01205	255976..256434	+	459	WP_001291990.1	xanthine phosphoribosyltransferase	-
-	257030..257110	+	81	NuclAT_12	-	-
-	257030..257110	+	81	NuclAT_12	-	-
-	257030..257110	+	81	NuclAT_12	-	-
-	257030..257110	+	81	NuclAT_12	-	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase toxin YafO (27-130)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 15487.84 Da
• Isoelectric Point: 7.4215

>T112092 WP_001263489.1 NZ_CP032679:252300-252698 [Escherichia coli str. K-12 substr. MG1655]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANENNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Nucleotide

• Length: 399 bp

>T112092 NZ_CP032679:252300-252698 [Escherichia coli str. K-12 substr. MG1655]
ATGCGGGTATTCAAAACAAAACTTATTCGCCTGCAACTTACAGCAGAGGAACTTGATGCGTTAACGGCGGATTTTATTTC
CTATAAGCGTGACGGTGTTTTGCCAGATATATTTGGTCGCGATGCACTCTACGACGACTCCTTTACCTGGCCATTAATCA
AATTTGAGCGAGTTGCTCATATTCATCTGGCAAATGAGAATAATCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACG
AATGACGAAGCGCATTTGGTATATTGTCAGGGGGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGA
ACCTCATAAACTGGCTCGAGATAACAACCAAATGCATAAAATTGGGAAAATGGCAGAAGCGTTTCGCATGCGTTTTTGA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11233.76 Da
• Isoelectric Point: 5.2170

>AT112092 WP_000554758.1 NZ_CP032679:252004-252297 [Escherichia coli str. K-12 substr. MG1655]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EQYLNDMTDDDFNDFKE

Nucleotide

• Length: 294 bp

>AT112092 NZ_CP032679:252004-252297 [Escherichia coli str. K-12 substr. MG1655]
ATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACC
GGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGATATCTCTTAAGTGCCAGCGCATTCGAAGCGTTAATGGACATGCTTG
CTGAACAAGAGGAGAAAAAGCCCATAAAGGCGCGCTTCCGTCCAAGTGCTGCAAGATTAGAGGAAATTACACGCCGCGCT
GAACAATATCTTAATGATATGACGGATGATGATTTCAATGACTTTAAGGAATAA

Structures

Toxin

• AlphaFold DB: A0A090J8B1

Antitoxin

• AlphaFold DB: S1QAE3