Detailed information of TA system TA112082

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): symER/SymE(toxin)
• Location: 3943267..3943679
• Replicon: chromosome
• Accession: NZ_CP032667
• Organism: Escherichia coli str. K-12 substr. MG1655 strain K-12

Toxin (Protein)

• Gene name: symE
• Uniprot ID: U9YSY7
• Locus tag: D8B36_RS19515
• Protein ID: WP_000132601.1
• Coordinates: 3943338..3943679 (+)
• Length: 114 a.a.

Antitoxin (RNA)

Gene name	symR
Locus tag	-
Coordinates	3943267..3943343 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
D8B36_RS19505	3940130..3941719	+	1590	WP_001063204.1	type I restriction-modification system methyltransferase	-
D8B36_RS19510	3941716..3943110	+	1395	WP_001272447.1	type I restriction-modification system specificity subunit	-
-	3943267..3943343	-	77	NuclAT_14	-	Antitoxin
-	3943267..3943343	-	77	NuclAT_14	-	Antitoxin
-	3943267..3943343	-	77	NuclAT_14	-	Antitoxin
-	3943267..3943343	-	77	NuclAT_14	-	Antitoxin
-	3943267..3943343	-	77	NuclAT_15	-	Antitoxin
-	3943267..3943343	-	77	NuclAT_15	-	Antitoxin
-	3943267..3943343	-	77	NuclAT_15	-	Antitoxin
-	3943267..3943343	-	77	NuclAT_15	-	Antitoxin
D8B36_RS19515	3943338..3943679	+	342	WP_000132601.1	endoribonuclease SymE	Toxin
D8B36_RS19520	3943841..3945220	+	1380	WP_000443951.1	5-methylcytosine-specific restriction endonuclease subunit McrB	-
D8B36_RS19525	3945220..3946266	+	1047	WP_000437621.1	5-methylcytosine-specific restriction endonuclease system specificity protein McrC	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin SymE-like (20-71)

Sequences

Toxin

Protein

• Length: 114 a.a.
• Molecular weight: 12203.02 Da
• Isoelectric Point: 8.5012

>T112082 WP_000132601.1 NZ_CP032667:3943338-3943679 [Escherichia coli str. K-12 substr. MG1655]
MTDTHSIAQPFEAEVSPANNRHVTVGYASRYPDYSRIPAITLKGQWLEAAGFATGTAVDVKVMEGCIVLTAQPPAAEESE
LMQSLRQVCKLSARKQKQVQAFIGVIAGKQKVA

Nucleotide

• Length: 342 bp

>T112082 NZ_CP032667:3943338-3943679 [Escherichia coli str. K-12 substr. MG1655]
ATGACTGACACGCATTCTATTGCACAACCGTTCGAAGCAGAAGTCTCCCCGGCAAATAACCGTCATGTCACCGTCGGTTA
TGCGAGTCGCTACCCGGATTACAGCCGTATTCCCGCCATCACCCTGAAAGGTCAGTGGCTGGAAGCCGCCGGTTTTGCCA
CTGGCACGGCGGTAGATGTCAAAGTGATGGAAGGCTGTATTGTCCTCACCGCCCAACCACCCGCCGCCGAGGAGAGCGAA
CTGATGCAGTCGCTACGCCAGGTGTGCAAACTGTCGGCGCGTAAACAAAAGCAGGTGCAGGCGTTTATTGGAGTGATTGC
CGGTAAACAGAAAGTCGCGTAA

Antitoxin

Nucleotide

• Length: 77 bp

>AT112082 NZ_CP032667:c3943343-3943267 [Escherichia coli str. K-12 substr. MG1655]
AGTCATAACTGCTATTCTCCAGGAATAGTGATTGTGATTAGCGATGCGGGTGTGTTGGCGCACATCCGCACCGCGCT

Structures

Toxin

• AlphaFold DB: U9YSY7

Antitoxin