112065

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-YefM
Location 1793659..1794161 Replicon chromosome
Accession NZ_CP032667
Organism Escherichia coli str. K-12 substr. MG1655 strain K-12

Toxin (Protein)


Gene name yoeB Uniprot ID U9YDH0
Locus tag D8B36_RS08695 Protein ID WP_000767829.1
Coordinates 1793907..1794161 (+) Length 85 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID S1Q063
Locus tag D8B36_RS08690 Protein ID WP_001259255.1
Coordinates 1793659..1793910 (+) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
D8B36_RS08660 1788837..1789904 - 1068 WP_000080105.1 bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB -
D8B36_RS08665 1789904..1790974 - 1071 WP_000108941.1 histidinol-phosphate transaminase -
D8B36_RS08670 1790971..1792275 - 1305 WP_000009594.1 histidinol dehydrogenase -
D8B36_RS08675 1792281..1793180 - 900 WP_000131782.1 ATP phosphoribosyltransferase -
D8B36_RS08685 1793326..1793376 - 51 WP_001364200.1 his operon leader peptide -
D8B36_RS08690 1793659..1793910 + 252 WP_001259255.1 YoeB-YefM toxin-antitoxin system antitoxin YefM Antitoxin
D8B36_RS08695 1793907..1794161 + 255 WP_000767829.1 type II toxin-antitoxin system mRNA interferase toxin YoeB Toxin
D8B36_RS08700 1794244..1795068 + 825 WP_000754737.1 SDR family oxidoreductase -
D8B36_RS08705 1795114..1796043 + 930 WP_000803351.1 LysR family transcriptional regulator -
D8B36_RS08710 1796258..1796320 + 63 WP_010723108.1 membrane protein YoeI -
D8B36_RS08715 1796310..1797668 + 1359 WP_000019197.1 putrescine/proton symporter PlaP -
D8B36_RS08720 1797847..1798905 + 1059 WP_000492339.1 transport protein YeeE -
D8B36_RS08725 1798919..1799146 + 228 WP_000234896.1 sulfurtransferase TusA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(2-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-73)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 10183.58 Da        Isoelectric Point: 8.0353

>T112065 WP_000767829.1 NZ_CP032667:1793907-1794161 [Escherichia coli str. K-12 substr. MG1655]
VKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAAC
RYHY

Download         Length: 255 bp

>T112065 NZ_CP032667:1793907-1794161 [Escherichia coli str. K-12 substr. MG1655]
GTGAAACTAATCTGGTCTGAGGAATCATGGGACGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACGCCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9307.58 Da        Isoelectric Point: 4.7954

>AT112065 WP_001259255.1 NZ_CP032667:1793659-1793910 [Escherichia coli str. K-12 substr. MG1655]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Download         Length: 252 bp

>AT112065 NZ_CP032667:1793659-1793910 [Escherichia coli str. K-12 substr. MG1655]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGCTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACGGAAAAGGAC
ATCATTGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6NY6
PDB 2A6S
PDB 6OXA
PDB 2A6R
PDB 4V8X
PDB 6OTR
PDB 6OXI
PDB 2A6Q

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9LNR0

References