Detailed information of TA system TA112039

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 6294406..6294999
• Replicon: chromosome
• Accession: NZ_CP032631
• Organism: Pseudomonas syringae pv. actinidiae str. Shaanxi_M228

Toxin (Protein)

• Gene name: graT
• Uniprot ID: A0A2G7PD43
• Locus tag: CN228_RS30475
• Protein ID: WP_003377943.1
• Coordinates: 6294406..6294684 (+)
• Length: 93 a.a.

Antitoxin (Protein)

• Gene name: graA
• Uniprot ID: Q88B19
• Locus tag: CN228_RS30480
• Protein ID: WP_005739893.1
• Coordinates: 6294694..6294999 (+)
• Length: 102 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CN228_RS30455	6289558..6290391	-	834	WP_005739898.1	transporter substrate-binding domain-containing protein	-
CN228_RS30460	6290419..6291291	-	873	WP_005613618.1	phytanoyl-CoA dioxygenase family protein	-
CN228_RS30465	6291316..6292836	-	1521	WP_024533279.1	amino acid ABC transporter permease/ATP-binding protein	-
CN228_RS30470	6293321..6294235	+	915	WP_019332092.1	LysR family transcriptional regulator	-
CN228_RS30475	6294406..6294684	+	279	WP_003377943.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
CN228_RS30480	6294694..6294999	+	306	WP_005739893.1	HigA family addiction module antidote protein	Antitoxin
CN228_RS30485	6295033..6295314	-	282	WP_017682769.1	accessory factor UbiK family protein	-
CN228_RS30495	6295734..6296072	+	339	WP_002555808.1	P-II family nitrogen regulator	-
CN228_RS30500	6296107..6297444	+	1338	WP_003377937.1	ammonium transporter	-
CN228_RS30505	6297432..6297695	-	264	WP_017699472.1	hypothetical protein	-
CN228_RS30510	6297687..6298112	+	426	WP_003377936.1	secondary thiamine-phosphate synthase enzyme YjbQ	-
CN228_RS30515	6298211..6298543	+	333	WP_003377934.1	hypothetical protein	-
CN228_RS30520	6298594..6299931	-	1338	WP_032685517.1	IS4-like element ISPsy33 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	6298594..6299949	1355

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (5-92)

Antitoxin

• Toxin-antitoxin system, antidote protein, HigA (6-84)

Sequences

Toxin

Protein

• Length: 93 a.a.
• Molecular weight: 10512.18 Da
• Isoelectric Point: 9.1490

>T112039 WP_003377943.1 NZ_CP032631:6294406-6294684 [Pseudomonas syringae pv. actinidiae str. Shaanxi_M228]
MIVSFKCVHTRYLFLQGKTRLWPSIKSVAERKLAMLDAATSILDLRSPPGNRLEVLDGSRSGQYSVRINAQFRICFVWSI
NGPEDVEIVDYH

Nucleotide

• Length: 279 bp

>T112039 NZ_CP032631:6294406-6294684 [Pseudomonas syringae pv. actinidiae str. Shaanxi_M228]
ATGATTGTGAGTTTTAAGTGCGTGCACACGAGATATCTCTTTTTACAAGGCAAGACGCGGTTGTGGCCATCGATTAAATC
TGTTGCCGAGCGCAAGCTCGCAATGCTTGATGCTGCTACCTCGATTTTGGATCTGCGCTCTCCTCCCGGCAATCGACTAG
AGGTGCTTGATGGAAGTCGTTCTGGCCAATACAGCGTACGTATAAACGCTCAATTCAGGATTTGCTTCGTTTGGAGCATC
AACGGTCCCGAGGATGTAGAAATTGTCGATTATCACTGA

Antitoxin

Protein

• Length: 102 a.a.
• Molecular weight: 11158.01 Da
• Isoelectric Point: 7.7509

>AT112039 WP_005739893.1 NZ_CP032631:6294694-6294999 [Pseudomonas syringae pv. actinidiae str. Shaanxi_M228]
MNKNGMRPVHPGEVLKEEYLEPMGLTAAALARALKVSTPTVNDIVLQRRGVSADVALRLAVCLETSPEFWLNLQLAYDLR
KAETEKGAQIREQVKCLAHCA

Nucleotide

• Length: 306 bp

>AT112039 NZ_CP032631:6294694-6294999 [Pseudomonas syringae pv. actinidiae str. Shaanxi_M228]
ATGAACAAAAACGGAATGCGCCCCGTCCATCCAGGCGAGGTTCTGAAAGAGGAATATCTTGAGCCGATGGGGCTGACCGC
CGCAGCGTTGGCCAGAGCCCTTAAGGTTTCTACGCCGACGGTGAACGACATCGTGCTCCAGCGCAGAGGTGTGAGCGCTG
ATGTAGCGCTGCGTCTGGCTGTATGCCTGGAGACGAGTCCTGAGTTCTGGCTTAACCTGCAGCTTGCCTACGATCTGCGC
AAGGCAGAGACAGAGAAGGGCGCGCAGATTCGTGAGCAGGTCAAATGTCTGGCCCACTGCGCCTGA

Structures

Toxin

• AlphaFold DB: A0A2G7PD43

Antitoxin

• AlphaFold DB: A0A0K8LYY6