Detailed information of TA system TA112011

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 2444717..2445389
• Replicon: chromosome
• Accession: NZ_CP032619
• Organism: Pectobacterium carotovorum strain HG-49

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: D7Z95_RS11055
• Protein ID: WP_180779426.1
• Coordinates: 2444717..2445103 (+)
• Length: 129 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: D7Z95_RS11060
• Protein ID: WP_180779427.1
• Coordinates: 2445090..2445389 (+)
• Length: 100 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
D7Z95_RS11030	2440630..2441772	+	1143	WP_180779423.1	FMNH2-dependent alkanesulfonate monooxygenase	-
D7Z95_RS11035	2441783..2442577	+	795	WP_180779424.1	aliphatic sulfonate ABC transporter permease SsuC	-
D7Z95_RS11040	2442574..2443374	+	801	WP_205947326.1	aliphatic sulfonates ABC transporter ATP-binding protein	-
D7Z95_RS11045	2443547..2443804	+	258	WP_012772768.1	DUF1471 domain-containing protein	-
D7Z95_RS11050	2444004..2444501	-	498	WP_205947325.1	GNAT family N-acetyltransferase	-
D7Z95_RS11055	2444717..2445103	+	387	WP_180779426.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
D7Z95_RS11060	2445090..2445389	+	300	WP_180779427.1	XRE family transcriptional regulator	Antitoxin
D7Z95_RS11065	2445426..2447225	-	1800	WP_205947324.1	ABC transporter ATP-binding protein	-
D7Z95_RS11070	2447265..2448173	-	909	WP_012772766.1	ABC transporter permease	-
D7Z95_RS11075	2448170..2449147	-	978	WP_012772765.1	ABC transporter permease	-
D7Z95_RS11080	2449147..2450154	-	1008	WP_205947323.1	nucleoside hydrolase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (19-113)

Antitoxin

• Cro/C1-type helix-turn-helix domain (32-80)

Sequences

Toxin

Protein

• Length: 129 a.a.
• Molecular weight: 14834.84 Da
• Isoelectric Point: 4.7331

>T112011 WP_180779426.1 NZ_CP032619:2444717-2445103 [Pectobacterium carotovorum]
MEVYRLAWEIVTRALFDTWFDEQTETVQEEVLAHLGILEEDGPQLGRPQVDQIKGSQYKNMKELRVQVGGHPFRTFFAFD
RTRKAIVLCAGDKKGENEKLFYERMIKIADAEFTSHLISPEVKEDGDI

Nucleotide

• Length: 387 bp

>T112011 NZ_CP032619:2444717-2445103 [Pectobacterium carotovorum]
ATGGAGGTTTACAGATTGGCATGGGAAATTGTCACACGGGCGCTATTCGATACGTGGTTTGATGAGCAAACCGAAACGGT
ACAGGAAGAGGTTTTGGCACACCTCGGTATACTCGAAGAAGATGGCCCACAGCTAGGACGACCACAGGTTGATCAGATTA
AAGGCTCACAATACAAAAATATGAAGGAGCTTCGAGTTCAGGTTGGCGGCCACCCATTCCGAACATTCTTCGCGTTTGAC
CGGACACGTAAAGCGATTGTTTTATGTGCGGGAGATAAGAAAGGCGAAAATGAAAAATTGTTCTACGAACGTATGATTAA
GATCGCAGACGCTGAGTTTACATCGCATCTGATATCACCGGAGGTAAAAGAAGATGGCGACATTTAA

Antitoxin

Protein

• Length: 100 a.a.
• Molecular weight: 11082.10 Da
• Isoelectric Point: 10.6442

>AT112011 WP_180779427.1 NZ_CP032619:2445090-2445389 [Pectobacterium carotovorum]
MATFKEMMAKRSPESRARIEARTAEIRQEITLAKLREELNMSQTQLALALGVKQPSIVKMEKVENDPKLSTLKRYVTALG
GKLTLDVTLPDGKRIALTL

Nucleotide

• Length: 300 bp

>AT112011 NZ_CP032619:2445090-2445389 [Pectobacterium carotovorum]
ATGGCGACATTTAAAGAAATGATGGCTAAACGTTCACCGGAGAGCCGCGCCCGCATTGAAGCGCGCACGGCTGAGATCCG
TCAGGAAATCACTTTAGCGAAACTACGCGAAGAGCTGAACATGTCGCAGACTCAGCTTGCATTAGCTCTGGGCGTCAAGC
AGCCATCTATTGTTAAGATGGAGAAGGTCGAGAATGACCCAAAACTGTCAACGCTGAAACGCTATGTCACAGCCCTAGGC
GGCAAGCTTACGCTAGATGTGACCTTGCCTGATGGTAAGCGTATCGCGCTTACTTTGTAA