Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 5212532..5213128 Replicon chromosome
Accession NZ_CP032616
Organism Pseudomonas sp. DY-1

Toxin (Protein)


Gene name higB Uniprot ID -
Locus tag D6Z43_RS24445 Protein ID WP_120654578.1
Coordinates 5212532..5212810 (+) Length 93 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID -
Locus tag D6Z43_RS24450 Protein ID WP_120654579.1
Coordinates 5212823..5213128 (+) Length 102 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
D6Z43_RS24410 5208226..5208678 + 453 WP_120654572.1 ribosomal protein S18-alanine N-acetyltransferase -
D6Z43_RS24415 5208750..5209394 + 645 WP_120654573.1 carbonate dehydratase -
D6Z43_RS24420 5209398..5210309 + 912 WP_120654574.1 DMT family transporter -
D6Z43_RS24425 5210314..5211312 + 999 WP_120654575.1 isocitrate/isopropylmalate dehydrogenase family protein -
D6Z43_RS24430 5211430..5211657 - 228 WP_120654576.1 hypothetical protein -
D6Z43_RS24435 5211788..5211991 - 204 WP_120654577.1 hypothetical protein -
D6Z43_RS24445 5212532..5212810 + 279 WP_120654578.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
D6Z43_RS24450 5212823..5213128 + 306 WP_120654579.1 HigA family addiction module antidote protein Antitoxin
D6Z43_RS24455 5213170..5213460 + 291 WP_120654580.1 excalibur calcium-binding domain-containing protein -
D6Z43_RS24460 5213765..5214043 + 279 WP_120654581.1 type II toxin-antitoxin system Phd/YefM family antitoxin -
D6Z43_RS24465 5214349..5216766 + 2418 WP_120654582.1 DNA cytosine methyltransferase -
D6Z43_RS24470 5216810..5217328 + 519 WP_120654583.1 DNA mismatch endonuclease Vsr -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-1

(4-92)

Antitoxin

Cro/C1-type helix-turn-helix domain

(18-68)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10752.17 Da        Isoelectric Point: 7.8668

>T111998 WP_120654578.1 NZ_CP032616:5212532-5212810 [Pseudomonas sp. DY-1]
VILSFRCQETRTLFETGDSRRWRNILTVATRKLVMLNAATELRDLRSPPGNRLEQLAENRAGQHSIRINDQWRICFEWTE
AGPANVEIVDYH

Download         Length: 279 bp

>T111998 NZ_CP032616:5212532-5212810 [Pseudomonas sp. DY-1]
GTGATTTTGAGCTTTCGATGTCAGGAAACTCGAACTCTGTTTGAAACGGGTGATTCGCGGCGCTGGAGAAACATCCTGAC
CGTCGCTACTCGAAAGCTGGTGATGCTCAACGCGGCCACTGAGCTTCGAGACTTGCGGTCCCCGCCCGGCAATCGGTTGG
AGCAGTTGGCTGAGAATAGGGCTGGGCAACACAGCATCAGGATCAACGACCAGTGGCGTATTTGCTTCGAGTGGACCGAG
GCAGGCCCTGCGAACGTTGAGATAGTCGATTACCACTGA

Antitoxin


Download         Length: 102 a.a.        Molecular weight: 11364.94 Da        Isoelectric Point: 4.5416

>AT111998 WP_120654579.1 NZ_CP032616:5212823-5213128 [Pseudomonas sp. DY-1]
MTINGMRPIHPGEILREEFLEPLDITPAALARALHVSAPTVNDIVRERRGITADMALRLGRYFDTTAQFWMNLQTEYALA
TTYAEAGEEIEHEIEPLRAVG

Download         Length: 306 bp

>AT111998 NZ_CP032616:5212823-5213128 [Pseudomonas sp. DY-1]
ATGACAATCAATGGCATGCGGCCCATTCATCCTGGAGAGATTCTGCGGGAAGAGTTCCTGGAGCCGCTGGACATCACACC
GGCCGCACTCGCTCGTGCGCTGCATGTGTCCGCGCCAACCGTGAACGATATCGTGCGTGAACGTCGCGGGATCACTGCTG
ACATGGCTCTGCGTCTGGGCCGTTACTTCGATACCACCGCCCAGTTCTGGATGAACCTGCAGACTGAGTACGCCTTGGCG
ACGACTTACGCTGAGGCTGGTGAAGAGATCGAGCACGAGATAGAGCCGCTGCGAGCGGTTGGATAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure

References