Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/RelE-HTH
Location 30764..31371 Replicon chromosome
Accession NZ_CP032616
Organism Pseudomonas sp. DY-1

Toxin (Protein)


Gene name higB Uniprot ID -
Locus tag D6Z43_RS00425 Protein ID WP_120649741.1
Coordinates 30764..31078 (+) Length 105 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID -
Locus tag D6Z43_RS00430 Protein ID WP_120649742.1
Coordinates 31084..31371 (+) Length 96 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
D6Z43_RS00405 26756..27082 + 327 WP_120649754.1 DUF2523 domain-containing protein -
D6Z43_RS00410 27095..28270 + 1176 WP_120649738.1 zonular occludens toxin -
D6Z43_RS00415 28436..29713 + 1278 WP_120649739.1 hypothetical protein -
D6Z43_RS00420 29695..30720 + 1026 WP_120649740.1 tyrosine-type recombinase/integrase -
D6Z43_RS00425 30764..31078 + 315 WP_120649741.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
D6Z43_RS00430 31084..31371 + 288 WP_120649742.1 helix-turn-helix domain-containing protein Antitoxin
D6Z43_RS00440 31636..32517 - 882 WP_120649760.1 alpha/beta hydrolase -
D6Z43_RS00445 32915..33607 - 693 WP_120649761.1 pseudouridine synthase -
D6Z43_RS00450 33607..33819 - 213 WP_120649762.1 cysteine-rich CWC family protein -
D6Z43_RS00455 34084..34806 + 723 WP_120649763.1 CDP-alcohol phosphatidyltransferase family protein -
D6Z43_RS00460 34977..35417 + 441 WP_120649764.1 carboxymuconolactone decarboxylase family protein -
D6Z43_RS00465 35425..35844 + 420 WP_120649765.1 cupin domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Integrative and Conjugative Element - - 18524..31490 12966


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-2

(43-100)

Antitoxin

Cro/C1-type helix-turn-helix domain

(35-86)


Sequences

Toxin        


Download         Length: 105 a.a.        Molecular weight: 11895.67 Da        Isoelectric Point: 10.0550

>T111995 WP_120649741.1 NZ_CP032616:30764-31078 [Pseudomonas sp. DY-1]
MIFIETPIFTKRLRELLSDDSYAAFQRQLAERPDMGDVIEGTGGIRKVRVASGGHGKRGGSRVIYYHFTSASQIALLLIY
PKNEKDDLSADERKVLKQIIERWR

Download         Length: 315 bp

>T111995 NZ_CP032616:30764-31078 [Pseudomonas sp. DY-1]
ATGATCTTTATTGAGACTCCGATCTTCACCAAGCGTCTGCGTGAGTTGCTCAGCGACGACAGCTATGCGGCGTTTCAGCG
CCAGTTGGCCGAGCGGCCGGATATGGGCGACGTGATCGAAGGCACCGGAGGTATTCGCAAAGTGCGTGTCGCCTCGGGTG
GACACGGTAAGCGGGGCGGGTCCAGGGTCATCTACTACCACTTCACTTCGGCTTCGCAGATTGCACTGCTGCTGATCTAC
CCGAAGAACGAGAAGGATGATCTGTCGGCCGATGAGCGAAAGGTGCTCAAGCAAATCATTGAGCGGTGGAGGTAA

Antitoxin


Download         Length: 96 a.a.        Molecular weight: 10862.45 Da        Isoelectric Point: 10.0108

>AT111995 WP_120649742.1 NZ_CP032616:31084-31371 [Pseudomonas sp. DY-1]
MSKFFEDLLESVQQMDEIHRGERKPSREFTVDALQVKEIRKATGLTQAKFAAMIDVQLGTLRNWEQGRREPTGPAKALLR
AIHNDPKHVIQALSH

Download         Length: 288 bp

>AT111995 NZ_CP032616:31084-31371 [Pseudomonas sp. DY-1]
ATGAGCAAATTCTTTGAAGACCTTCTGGAAAGCGTCCAGCAAATGGACGAGATCCACCGTGGCGAGCGTAAGCCTTCACG
GGAGTTCACCGTGGACGCGCTGCAGGTGAAGGAAATCCGCAAGGCCACTGGCCTGACCCAAGCCAAGTTTGCGGCCATGA
TCGATGTGCAGCTCGGCACGTTGCGCAACTGGGAACAAGGTCGGCGTGAACCCACTGGCCCGGCCAAGGCGCTGCTGCGG
GCCATCCACAATGACCCCAAACACGTCATCCAGGCACTGTCTCACTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure

References