Detailed information of TA system TA111993

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/RelE-HTH
• Location: 17986..18593
• Replicon: plasmid p.1
• Accession: NZ_CP032615
• Organism: Pseudomonas sp. DY-1

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: D6Z43_RS00130
• Protein ID: WP_120649741.1
• Coordinates: 17986..18300 (+)
• Length: 105 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: D6Z43_RS00135
• Protein ID: WP_120649742.1
• Coordinates: 18306..18593 (+)
• Length: 96 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
D6Z43_RS00110	13978..14304	+	327	WP_120649754.1	DUF2523 domain-containing protein	-
D6Z43_RS00115	14317..15492	+	1176	WP_120649738.1	zonular occludens toxin	-
D6Z43_RS00120	15658..16935	+	1278	WP_120649739.1	hypothetical protein	-
D6Z43_RS00125	16917..17942	+	1026	WP_120649740.1	tyrosine-type recombinase/integrase	-
D6Z43_RS00130	17986..18300	+	315	WP_120649741.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
D6Z43_RS00135	18306..18593	+	288	WP_120649742.1	helix-turn-helix domain-containing protein	Antitoxin
D6Z43_RS00140	18918..19670	+	753	WP_120649743.1	hypothetical protein	-
D6Z43_RS00145	19824..21011	-	1188	WP_120649744.1	hypothetical protein	-
D6Z43_RS00150	21020..22198	-	1179	WP_120649745.1	hypothetical protein	-
D6Z43_RS00155	22338..22613	-	276	WP_120649746.1	helix-turn-helix transcriptional regulator	-
D6Z43_RS00160	22741..22956	+	216	WP_120649747.1	DNA-binding protein	-
D6Z43_RS00165	22953..23243	+	291	WP_120649748.1	hypothetical protein	-
D6Z43_RS00170	23247..23471	+	225	WP_120649755.1	DNA-binding protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..26350	26350

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-2 (43-100)

Antitoxin

• Cro/C1-type helix-turn-helix domain (35-86)

Sequences

Toxin

Protein

• Length: 105 a.a.
• Molecular weight: 11895.67 Da
• Isoelectric Point: 10.0550

>T111993 WP_120649741.1 NZ_CP032615:17986-18300 [Pseudomonas sp. DY-1]
MIFIETPIFTKRLRELLSDDSYAAFQRQLAERPDMGDVIEGTGGIRKVRVASGGHGKRGGSRVIYYHFTSASQIALLLIY
PKNEKDDLSADERKVLKQIIERWR

Nucleotide

• Length: 315 bp

>T111993 NZ_CP032615:17986-18300 [Pseudomonas sp. DY-1]
ATGATCTTTATTGAGACTCCGATCTTCACCAAGCGTCTGCGTGAGTTGCTCAGCGACGACAGCTATGCGGCGTTTCAGCG
CCAGTTGGCCGAGCGGCCGGATATGGGCGACGTGATCGAAGGCACCGGAGGTATTCGCAAAGTGCGTGTCGCCTCGGGTG
GACACGGTAAGCGGGGCGGGTCCAGGGTCATCTACTACCACTTCACTTCGGCTTCGCAGATTGCACTGCTGCTGATCTAC
CCGAAGAACGAGAAGGATGATCTGTCGGCCGATGAGCGAAAGGTGCTCAAGCAAATCATTGAGCGGTGGAGGTAA

Antitoxin

Protein

• Length: 96 a.a.
• Molecular weight: 10862.45 Da
• Isoelectric Point: 10.0108

>AT111993 WP_120649742.1 NZ_CP032615:18306-18593 [Pseudomonas sp. DY-1]
MSKFFEDLLESVQQMDEIHRGERKPSREFTVDALQVKEIRKATGLTQAKFAAMIDVQLGTLRNWEQGRREPTGPAKALLR
AIHNDPKHVIQALSH

Nucleotide

• Length: 288 bp

>AT111993 NZ_CP032615:18306-18593 [Pseudomonas sp. DY-1]
ATGAGCAAATTCTTTGAAGACCTTCTGGAAAGCGTCCAGCAAATGGACGAGATCCACCGTGGCGAGCGTAAGCCTTCACG
GGAGTTCACCGTGGACGCGCTGCAGGTGAAGGAAATCCGCAAGGCCACTGGCCTGACCCAAGCCAAGTTTGCGGCCATGA
TCGATGTGCAGCTCGGCACGTTGCGCAACTGGGAACAAGGTCGGCGTGAACCCACTGGCCCGGCCAAGGCGCTGCTGCGG
GCCATCCACAATGACCCCAAACACGTCATCCAGGCACTGTCTCACTGA