Detailed information of TA system TA111992

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/RelE-HTH
• Location: 5215..5822
• Replicon: plasmid p.1
• Accession: NZ_CP032615
• Organism: Pseudomonas sp. DY-1

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: D6Z43_RS00035
• Protein ID: WP_120649741.1
• Coordinates: 5215..5529 (+)
• Length: 105 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: D6Z43_RS00040
• Protein ID: WP_120649742.1
• Coordinates: 5535..5822 (+)
• Length: 96 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
D6Z43_RS27725	500..808	+	309	WP_120649759.1	hypothetical protein	-
D6Z43_RS00010	840..1208	+	369	WP_162945784.1	hypothetical protein	-
D6Z43_RS00015	1208..1533	+	326	Protein_3	DUF2523 domain-containing protein	-
D6Z43_RS00020	1546..2721	+	1176	WP_120649738.1	zonular occludens toxin	-
D6Z43_RS00025	2887..4164	+	1278	WP_120649739.1	hypothetical protein	-
D6Z43_RS00030	4146..5171	+	1026	WP_120649740.1	tyrosine-type recombinase/integrase	-
D6Z43_RS00035	5215..5529	+	315	WP_120649741.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
D6Z43_RS00040	5535..5822	+	288	WP_120649742.1	helix-turn-helix domain-containing protein	Antitoxin
D6Z43_RS00045	6147..6899	+	753	WP_120649743.1	hypothetical protein	-
D6Z43_RS00050	7054..8241	-	1188	WP_120649744.1	hypothetical protein	-
D6Z43_RS00055	8250..9428	-	1179	WP_120649745.1	hypothetical protein	-
D6Z43_RS00060	9568..9843	-	276	WP_120649746.1	helix-turn-helix transcriptional regulator	-
D6Z43_RS00065	9971..10186	+	216	WP_120649747.1	DNA-binding protein	-
D6Z43_RS00070	10183..10473	+	291	WP_120649748.1	hypothetical protein	-
D6Z43_RS00075	10533..10700	+	168	WP_120649749.1	DNA-binding protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..26350	26350

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-2 (43-100)

Antitoxin

• Cro/C1-type helix-turn-helix domain (35-86)

Sequences

Toxin

Protein

• Length: 105 a.a.
• Molecular weight: 11895.67 Da
• Isoelectric Point: 10.0550

>T111992 WP_120649741.1 NZ_CP032615:5215-5529 [Pseudomonas sp. DY-1]
MIFIETPIFTKRLRELLSDDSYAAFQRQLAERPDMGDVIEGTGGIRKVRVASGGHGKRGGSRVIYYHFTSASQIALLLIY
PKNEKDDLSADERKVLKQIIERWR

Nucleotide

• Length: 315 bp

>T111992 NZ_CP032615:5215-5529 [Pseudomonas sp. DY-1]
ATGATCTTTATTGAGACTCCGATCTTCACCAAGCGTCTGCGTGAGTTGCTCAGCGACGACAGCTATGCGGCGTTTCAGCG
CCAGTTGGCCGAGCGGCCGGATATGGGCGACGTGATCGAAGGCACCGGAGGTATTCGCAAAGTGCGTGTCGCCTCGGGTG
GACACGGTAAGCGGGGCGGGTCCAGGGTCATCTACTACCACTTCACTTCGGCTTCGCAGATTGCACTGCTGCTGATCTAC
CCGAAGAACGAGAAGGATGATCTGTCGGCCGATGAGCGAAAGGTGCTCAAGCAAATCATTGAGCGGTGGAGGTAA

Antitoxin

Protein

• Length: 96 a.a.
• Molecular weight: 10862.45 Da
• Isoelectric Point: 10.0108

>AT111992 WP_120649742.1 NZ_CP032615:5535-5822 [Pseudomonas sp. DY-1]
MSKFFEDLLESVQQMDEIHRGERKPSREFTVDALQVKEIRKATGLTQAKFAAMIDVQLGTLRNWEQGRREPTGPAKALLR
AIHNDPKHVIQALSH

Nucleotide

• Length: 288 bp

>AT111992 NZ_CP032615:5535-5822 [Pseudomonas sp. DY-1]
ATGAGCAAATTCTTTGAAGACCTTCTGGAAAGCGTCCAGCAAATGGACGAGATCCACCGTGGCGAGCGTAAGCCTTCACG
GGAGTTCACCGTGGACGCGCTGCAGGTGAAGGAAATCCGCAAGGCCACTGGCCTGACCCAAGCCAAGTTTGCGGCCATGA
TCGATGTGCAGCTCGGCACGTTGCGCAACTGGGAACAAGGTCGGCGTGAACCCACTGGCCCGGCCAAGGCGCTGCTGCGG
GCCATCCACAATGACCCCAAACACGTCATCCAGGCACTGTCTCACTGA