Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 5307244..5307839 Replicon chromosome
Accession NZ_CP032569
Organism Pseudomonas aeruginosa strain BA7823

Toxin (Protein)


Gene name higB Uniprot ID V6ALY3
Locus tag D7H65_RS25320 Protein ID WP_003113526.1
Coordinates 5307561..5307839 (-) Length 93 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID A0A0S3KUN4
Locus tag D7H65_RS25315 Protein ID WP_003111575.1
Coordinates 5307244..5307549 (-) Length 102 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
D7H65_RS25280 5302385..5303233 + 849 WP_031686602.1 4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol kinase -
D7H65_RS25290 5303400..5304341 + 942 WP_003099281.1 ribose-phosphate pyrophosphokinase -
D7H65_RS25295 5304458..5305072 + 615 WP_003095013.1 50S ribosomal protein L25/general stress protein Ctc -
D7H65_RS25300 5305114..5305698 + 585 WP_003099278.1 aminoacyl-tRNA hydrolase -
D7H65_RS25305 5305739..5306839 + 1101 WP_003099270.1 redox-regulated ATPase YchF -
D7H65_RS25315 5307244..5307549 - 306 WP_003111575.1 HigA family addiction module antidote protein Antitoxin
D7H65_RS25320 5307561..5307839 - 279 WP_003113526.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
D7H65_RS25330 5308168..5310396 + 2229 WP_033991161.1 TonB-dependent receptor -
D7H65_RS25335 5310467..5311114 - 648 WP_003095021.1 carbonate dehydratase -
D7H65_RS25340 5311176..5312414 - 1239 WP_031686604.1 C69 family dipeptidase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-1

(5-92)

Antitoxin

Toxin-antitoxin system, antidote protein, HigA

(6-82)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10646.19 Da        Isoelectric Point: 7.8937

>T111987 WP_003113526.1 NZ_CP032569:c5307839-5307561 [Pseudomonas aeruginosa]
MILTFRCDETRQLFETGLSRRWGAILTVATRKLAMLHAATELRDLRSPPGNRLEPLQGKRAGQHSIRINDQWRVCFVWTD
AGPEEVEIVDYH

Download         Length: 279 bp

>T111987 NZ_CP032569:c5307839-5307561 [Pseudomonas aeruginosa]
ATGATTCTGACCTTTCGCTGCGACGAGACTCGTCAGCTTTTTGAGACGGGTCTTTCGAGACGGTGGGGAGCGATCCTCAC
AGTCGCTACGCGTAAGCTCGCAATGCTTCATGCGGCTACGGAGCTTCGAGACTTGCGCTCTCCACCTGGAAACCGGTTGG
AGCCGTTGCAGGGAAAGCGGGCGGGCCAACATAGCATCAGGATCAATGACCAGTGGCGTGTCTGTTTCGTCTGGACGGAT
GCGGGTCCCGAAGAAGTCGAAATAGTTGATTACCACTGA

Antitoxin


Download         Length: 102 a.a.        Molecular weight: 11205.72 Da        Isoelectric Point: 4.4475

>AT111987 WP_003111575.1 NZ_CP032569:c5307549-5307244 [Pseudomonas aeruginosa]
MATNGMRPIHPGEILRDEFLMELDISPAALARALKVSAPTVNDIVREQRGISADMAIRLGRYFDTSAQFWMNLQSEYSLA
TAYAANGEQIEHEIEPLLAQG

Download         Length: 306 bp

>AT111987 NZ_CP032569:c5307549-5307244 [Pseudomonas aeruginosa]
ATGGCTACCAATGGTATGCGCCCCATCCATCCTGGGGAAATATTGCGCGATGAGTTTCTGATGGAGTTGGATATCTCTCC
AGCTGCTCTAGCACGCGCTTTGAAAGTCTCCGCTCCGACAGTGAATGATATCGTTCGTGAGCAGCGTGGTATCTCCGCAG
ATATGGCGATTCGTCTGGGGCGTTACTTTGATACGTCCGCTCAGTTCTGGATGAATCTCCAGAGTGAGTATTCGTTAGCA
ACTGCTTATGCGGCGAATGGCGAGCAGATCGAACATGAGATTGAGCCGCTGCTTGCTCAGGGATAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB V6ALY3

Antitoxin

Source ID Structure
AlphaFold DB A0A0S3KUN4

References