Detailed information of TA system TA111960

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-VapI
• Location: 849715..850370
• Replicon: chromosome
• Accession: NZ_CP032542
• Organism: Coxiella burnetii strain 2014-PE-15890

Toxin (Protein)

• Gene name: graT
• Uniprot ID: Q83BL3
• Locus tag: D6219_RS04960
• Protein ID: WP_010958264.1
• Coordinates: 849715..850053 (+)
• Length: 113 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: D6219_RS04965
• Protein ID: WP_010958263.1
• Coordinates: 850059..850370 (+)
• Length: 104 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
D6219_RS04940	844787..845806	-	1020	WP_040953503.1	IS110-like element IS1111A family transposase	-
D6219_RS04950	846210..847883	-	1674	WP_012220680.1	CBU_1493 family Dot/Icm T4SS effector	-
D6219_RS04955	848211..849356	-	1146	WP_040953472.1	ISAs1 family transposase	-
D6219_RS04960	849715..850053	+	339	WP_010958264.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
D6219_RS04965	850059..850370	+	312	WP_010958263.1	HigA family addiction module antidote protein	Antitoxin
D6219_RS04970	850439..851170	-	732	WP_010958262.1	UDP-2,3-diacylglucosamine diphosphatase	-
D6219_RS04975	851327..852724	+	1398	WP_010958261.1	glutamate--tRNA ligase	-
D6219_RS04980	852715..854097	+	1383	WP_010958260.1	cysteine--tRNA ligase	-
D6219_RS04985	854193..855212	+	1020	WP_129545665.1	IS110-like element IS1111A family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	IScluster/Tn	-	CBU_1493	844787..867691	22904

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (23-112)

Antitoxin

• Cro/C1-type helix-turn-helix domain (26-72)

Sequences

Toxin

Protein

• Length: 113 a.a.
• Molecular weight: 13409.66 Da
• Isoelectric Point: 10.4588

>T111960 WP_010958264.1 NZ_CP032542:849715-850053 [Coxiella burnetii]
MPLTLYIMLDVTRKTVILEVIIKSFKDKYTKYLYKGVSVSKWQAIRKQAERRLQILDSVTSLDDLRSLPSNRFESLRGNR
KGQFSIRINKQWRICFKWINNEPTEVEIVDYH

Nucleotide

• Length: 339 bp

>T111960 NZ_CP032542:849715-850053 [Coxiella burnetii]
TTGCCTCTTACGCTGTATATTATGCTTGATGTAACGCGTAAAACTGTTATCCTTGAAGTGATAATAAAAAGTTTCAAGGA
CAAATACACTAAATATTTATATAAAGGTGTATCCGTTTCAAAATGGCAAGCCATACGAAAGCAGGCGGAAAGACGGCTTC
AGATTTTGGATTCAGTGACATCGTTAGATGATTTGAGAAGTTTGCCAAGCAATCGTTTTGAATCGTTGCGGGGGAATAGA
AAAGGACAATTTAGTATAAGGATTAATAAGCAATGGCGCATTTGTTTTAAGTGGATTAACAATGAGCCAACTGAAGTTGA
AATTGTGGATTATCATTAG

Antitoxin

Protein

• Length: 104 a.a.
• Molecular weight: 11458.39 Da
• Isoelectric Point: 10.1699

>AT111960 WP_010958263.1 NZ_CP032542:850059-850370 [Coxiella burnetii]
MKMAANRMRPIHPGEILAEELGFLDKMSANQLAKHLAIPTNRVTAILNGARSITADTALRLAKFFGTTPEFWLNLQDAYD
IKMALKKSGKKIEKEVTPYDQAA

Nucleotide

• Length: 312 bp

>AT111960 NZ_CP032542:850059-850370 [Coxiella burnetii]
ATGAAAATGGCAGCTAATAGAATGCGACCCATACATCCAGGTGAGATCCTTGCTGAAGAGTTGGGGTTTTTAGATAAGAT
GTCAGCTAATCAGTTAGCTAAACATTTAGCAATTCCGACCAACCGAGTGACCGCCATTCTCAATGGCGCGCGATCCATTA
CGGCGGATACCGCGCTACGATTGGCTAAGTTTTTTGGGACGACGCCGGAATTTTGGCTAAATCTCCAAGACGCCTATGAC
ATAAAAATGGCATTGAAAAAGTCCGGCAAAAAAATCGAAAAAGAAGTAACCCCTTACGACCAAGCCGCCTAG

Structures

Toxin

• AlphaFold DB: Q83BL3