Detailed information of TA system    

insolicoBioinformatically predicted

Overview


TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-YefM
Location 4465679..4466181 Replicon chromosome
Accession NZ_CP032523
Organism Shigella sonnei strain AR_0030

Toxin (Protein)


Gene name yoeB Uniprot ID U9YDH0
Locus tag AM357_RS23275 Protein ID WP_000767829.1
Coordinates 4465679..4465933 (-) Length 85 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID S1Q063
Locus tag AM357_RS23280 Protein ID WP_001259255.1
Coordinates 4465930..4466181 (-) Length 84 a.a.

Genomic Context


Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AM357_RS23245 (4460694) 4460694..4460921 - 228 WP_000985273.1 thiosulfate utilization sulfurtransferase TsuB/YeeD -
AM357_RS23250 (4460935) 4460935..4461993 - 1059 WP_000492295.1 thiosulfate utilization transporter TsuA/YeeE -
AM357_RS23255 (4462172) 4462172..4463530 - 1359 WP_000019197.1 putrescine/proton symporter PlaP -
AM357_RS23260 (4463520) 4463520..4463582 - 63 WP_010723108.1 membrane protein YoeI -
AM357_RS23265 (4463797) 4463797..4464726 - 930 WP_005149930.1 LysR substrate-binding domain-containing protein -
AM357_RS23270 (4464772) 4464772..4465596 - 825 WP_000754737.1 SDR family oxidoreductase -
AM357_RS23275 (4465679) 4465679..4465933 - 255 WP_000767829.1 type II toxin-antitoxin system mRNA interferase toxin YoeB Toxin
AM357_RS23280 (4465930) 4465930..4466181 - 252 WP_001259255.1 YoeB-YefM toxin-antitoxin system antitoxin YefM Antitoxin
AM357_RS23285 (4466464) 4466464..4466514 + 51 WP_001364200.1 his operon leader peptide -
AM357_RS23290 (4466660) 4466660..4467559 + 900 WP_000131782.1 ATP phosphoribosyltransferase -
AM357_RS23295 (4467565) 4467565..4468869 + 1305 WP_001462576.1 histidinol dehydrogenase -
AM357_RS23300 (4468866) 4468866..4469936 + 1071 WP_000108977.1 histidinol-phosphate transaminase -
AM357_RS23305 (4469936) 4469936..4471003 + 1068 WP_000080086.1 bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB -

Associated MGEs


MGE
detail
Similar
MGEs
Relative
position
MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains


Predicted by InterproScan

Toxin

(2-84)

Antitoxin

(1-73)


Sequences


Toxin        


Download         Length: 85 a.a.        Molecular weight: 10183.58 Da        Isoelectric Point: 8.0353

>T111950 WP_000767829.1 NZ_CP032523:c4465933-4465679 [Shigella sonnei]
VKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAAC
RYHY

Download         Length: 255 bp

>T111950 NZ_CP032523:c4465933-4465679 [Shigella sonnei]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACGCCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9307.58 Da        Isoelectric Point: 4.7954

>AT111950 WP_001259255.1 NZ_CP032523:c4466181-4465930 [Shigella sonnei]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Download         Length: 252 bp

>AT111950 NZ_CP032523:c4466181-4465930 [Shigella sonnei]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGCTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACAGAAAAGGAC
ATTATTGAGTGA

Similar Proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures


Toxin

Source ID Structure
PDB 6NY6
PDB 2A6S
PDB 6OXA
PDB 2A6R
PDB 4V8X
PDB 6OTR
PDB 6OXI
PDB 2A6Q


Antitoxin

Source ID Structure
PDB 2A6Q
AlphaFold DB A0A7U9LNR0

References