Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 769592..770319 Replicon chromosome
Accession NZ_CP032523
Organism Shigella sonnei strain AR_0030

Toxin (Protein)


Gene name higB Uniprot ID U9ZMR4
Locus tag AM357_RS04140 Protein ID WP_000550189.1
Coordinates 770005..770319 (-) Length 105 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID -
Locus tag AM357_RS04135 Protein ID WP_000560263.1
Coordinates 769592..770008 (-) Length 139 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AM357_RS04125 (764952) 764952..767303 + 2352 WP_000695472.1 alpha-glucosidase -
AM357_RS04130 (767529) 767529..769547 + 2019 WP_000121451.1 NADPH-dependent 2,4-dienoyl-CoA reductase -
AM357_RS04135 (769592) 769592..770008 - 417 WP_000560263.1 type II toxin-antitoxin system antitoxin HigA Antitoxin
AM357_RS04140 (770005) 770005..770319 - 315 WP_000550189.1 type II toxin-antitoxin system toxin HigB Toxin
AM357_RS04145 (770604) 770604..771740 - 1137 WP_000018685.1 23S rRNA (guanine(1835)-N(2))-methyltransferase RlmG -
AM357_RS04150 (771825) 771825..772328 + 504 WP_001305117.1 M48 family metallopeptidase -
AM357_RS04155 (772405) 772405..773088 + 684 WP_001183060.1 vancomycin high temperature exclusion protein -
AM357_RS04160 (773167) 773167..774153 + 987 WP_001446103.1 Gfo/Idh/MocA family oxidoreductase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, mRNA interferase HigB

(20-92)

Antitoxin

Cro/C1-type helix-turn-helix domain

(84-135)


Sequences

Toxin        


Download         Length: 105 a.a.        Molecular weight: 12103.10 Da        Isoelectric Point: 10.0409

>T111934 WP_000550189.1 NZ_CP032523:c770319-770005 [Shigella sonnei]
MHLITQKALKDAAEKYPQHKTELVALGNTIAKGYFKKPESLKAVFPSLDNFKYLDKHYVFNVGGNELRVVAMVFFESQKC
YIREVMTHKEYDFFTAVHRTKGKK

Download         Length: 315 bp

>T111934 NZ_CP032523:c770319-770005 [Shigella sonnei]
ATGCACCTGATAACTCAAAAAGCATTGAAAGATGCTGCGGAAAAATACCCGCAACATAAAACGGAGTTGGTGGCTCTGGG
GAACACGATTGCTAAGGGATATTTCAAAAAACCTGAGTCATTAAAAGCAGTATTCCCATCTCTGGATAACTTCAAATATC
TGGATAAGCATTATGTTTTCAATGTTGGGGGCAATGAATTACGTGTTGTAGCAATGGTCTTTTTTGAATCGCAAAAGTGC
TACATACGTGAAGTTATGACGCATAAAGAATACGATTTCTTTACCGCTGTTCATCGTACTAAGGGGAAAAAATGA

Antitoxin


Download         Length: 139 a.a.        Molecular weight: 14979.42 Da        Isoelectric Point: 4.4547

>AT111934 WP_000560263.1 NZ_CP032523:c770008-769592 [Shigella sonnei]
MIAIADILQAGEKLTAVAPFLAGIQNEEQYTQALELVDHLLLNDPENPLLDLVCAKITAWEESAPEFAEFNAMAQAMPGG
IAVIRTLMDQYGLTLADLPEIGSKSMVSRVLSGKRKLTLEHAKKLATRFGISPALFID

Download         Length: 417 bp

>AT111934 NZ_CP032523:c770008-769592 [Shigella sonnei]
ATGATTGCGATTGCCGACATCTTGCAAGCAGGAGAAAAGCTAACTGCGGTGGCACCTTTTCTGGCGGGTATTCAGAACGA
GGAACAATACACCCAGGCGCTGGAACTGGTAGATCATCTGCTGCTCAACGATCCTGAAAACCCCTTGCTGGATCTGGTGT
GTGCCAAAATAACCGCGTGGGAAGAATCAGCGCCCGAATTTGCGGAATTTAATGCCATGGCTCAAGCCATGCCTGGCGGT
ATAGCCGTGATTCGTACCCTTATGGATCAATATGGTTTAACCCTTGCCGATCTGCCGGAAATTGGCAGTAAATCTATGGT
GTCACGCGTTTTGAGCGGGAAGAGGAAATTAACGCTGGAACACGCTAAAAAATTGGCAACGCGATTCGGCATTTCTCCCG
CCTTGTTTATTGATTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6KMQ
PDB 5IFG
PDB 5YCL
PDB 6KML

Antitoxin

Source ID Structure

References