Detailed information of TA system TA111931

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 307972..308699
• Replicon: chromosome
• Accession: NZ_CP032523
• Organism: Shigella sonnei strain AR_0030

Toxin (Protein)

• Gene name: higB
• Uniprot ID: Q3YYE6
• Locus tag: AM357_RS01720
• Protein ID: WP_000547563.1
• Coordinates: 308388..308699 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: AM357_RS01715
• Protein ID: WP_000126296.1
• Coordinates: 307972..308391 (-)
• Length: 140 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AM357_RS01695 (303024)	303024..303551	-	528	WP_001078777.1	electron transport protein HydN	-
AM357_RS01700 (303698)	303698..304708	-	1011	WP_001402444.1	DNA-binding transcriptional regulator AscG	-
AM357_RS01705 (304968)	304968..306425	+	1458	WP_001107890.1	PTS cellobiose/arbutin/salicin transporter subunit IIBC	-
AM357_RS01710 (306434)	306434..307858	+	1425	WP_119170825.1	6-phospho-beta-glucosidase AscB	-
AM357_RS01715 (307972)	307972..308391	-	420	WP_000126296.1	helix-turn-helix domain-containing protein	Antitoxin
AM357_RS01720 (308388)	308388..308699	-	312	WP_000547563.1	type II toxin-antitoxin system HigB family toxin	Toxin
AM357_RS01725 (308861)	308861..309634	-	774	WP_001026444.1	hypothetical protein	-
AM357_RS01730 (309659)	309659..310129	-	471	WP_000132961.1	hydrogenase maturation peptidase HycI	-
AM357_RS01735 (310122)	310122..310532	-	411	WP_001291918.1	formate hydrogenlyase assembly protein HycH	-
AM357_RS01740 (310529)	310529..311296	-	768	WP_000067403.1	formate hydrogenlyase subunit HycG	-
AM357_RS01745 (311296)	311296..311838	-	543	WP_000493785.1	formate hydrogenlyase subunit HycF	-
AM357_RS01750 (311848)	311848..313545	-	1698	WP_001288143.1	formate hydrogenlyase subunit HycE	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, mRNA interferase HigB (22-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (85-138)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12398.11 Da
• Isoelectric Point: 9.7105

>T111931 WP_000547563.1 NZ_CP032523:c308699-308388 [Shigella sonnei]
MHIISKAPFEESARKYPNDALALQALYRVIKETDFSTPEEMRTAFPNLDNFKYRNKWYVLDVGGNNLRVIAYINFVNKRF
FVKHITNHAEYDKLTRYYRENKE

Nucleotide

• Length: 312 bp

>T111931 NZ_CP032523:c308699-308388 [Shigella sonnei]
ATGCACATCATTTCTAAAGCGCCATTTGAAGAGTCTGCGCGTAAATATCCTAACGACGCTCTGGCTCTTCAGGCATTGTA
TCGGGTTATTAAGGAAACGGATTTTTCTACGCCAGAAGAGATGCGAACGGCGTTTCCCAACCTCGACAATTTTAAATATC
GCAACAAATGGTACGTTCTGGATGTAGGTGGGAACAACCTAAGGGTGATTGCCTACATAAACTTTGTTAATAAGCGGTTC
TTTGTGAAGCACATAACTAATCACGCCGAGTATGACAAACTGACACGTTACTACAGAGAGAACAAAGAATGA

Antitoxin

Protein

• Length: 140 a.a.
• Molecular weight: 15416.38 Da
• Isoelectric Point: 4.3653

>AT111931 WP_000126296.1 NZ_CP032523:c308391-307972 [Shigella sonnei]
MTANAARAVKATRELVNAVPFLGGSDSEDDYREALELVEYLIEEDDTNPLIDFLASRIAEYENNNQEFAEFDKAVAAMPV
GVALLRTLIDQHNLTYADLKNEIGSKSLVSQILSGQRSLTISHIKALSARFGVKPEWFL

Nucleotide

• Length: 420 bp

>AT111931 NZ_CP032523:c308391-307972 [Shigella sonnei]
ATGACTGCTAATGCTGCACGCGCTGTTAAAGCAACAAGAGAACTGGTCAATGCAGTCCCCTTTCTGGGAGGTAGTGACTC
AGAAGATGATTATCGTGAAGCACTGGAACTGGTCGAATATTTGATTGAAGAAGATGACACCAATCCACTTATCGACTTTC
TGGCGAGCCGCATTGCAGAATATGAAAACAACAACCAAGAGTTTGCAGAGTTCGACAAAGCCGTTGCGGCAATGCCTGTT
GGCGTGGCGTTACTTCGCACGCTGATTGATCAACATAATCTGACCTATGCGGATTTGAAGAACGAAATCGGCTCTAAATC
CCTGGTCAGTCAGATTTTGTCTGGACAACGATCCCTGACGATATCCCACATTAAGGCACTTTCAGCCCGATTTGGCGTTA
AGCCGGAATGGTTTCTTTGA

Structures

Toxin

• AlphaFold DB: A0A7U9QA22