Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 644734..645352 Replicon chromosome
Accession NZ_CP032518
Organism Cupriavidus oxalaticus strain T2

Toxin (Protein)


Gene name graT Uniprot ID A0A375GMD0
Locus tag D2917_RS02805 Protein ID WP_063240603.1
Coordinates 644734..645012 (+) Length 93 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID A0A375GIG3
Locus tag D2917_RS02810 Protein ID WP_063240604.1
Coordinates 645023..645352 (+) Length 110 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
D2917_RS02805 644734..645012 + 279 WP_063240603.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
D2917_RS02810 645023..645352 + 330 WP_063240604.1 HigA family addiction module antidote protein Antitoxin
D2917_RS02815 645442..646038 - 597 WP_063240605.1 GTP cyclohydrolase II -
D2917_RS02820 646219..646689 - 471 WP_063240606.1 Lrp/AsnC family transcriptional regulator -
D2917_RS02825 646842..647204 + 363 WP_151069509.1 AsnC family transcriptional regulator -
D2917_RS02830 647214..648131 - 918 WP_151069510.1 LysR family transcriptional regulator -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-1

(28-92)

Antitoxin

Toxin-antitoxin system, antidote protein, HigA

(6-94)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10566.15 Da        Isoelectric Point: 8.5135

>T111922 WP_063240603.1 NZ_CP032518:644734-645012 [Cupriavidus oxalaticus]
MIQSFRCFDTAALFDGRYVARFASIEFIAQRKLEMLDDAATLGALRFPPGNRLEKLGGDRKGRYSIRINGQWRICFIWTH
DGPAAVEIIDYH

Download         Length: 279 bp

>T111922 NZ_CP032518:644734-645012 [Cupriavidus oxalaticus]
ATGATCCAATCGTTCAGATGCTTTGACACCGCTGCGTTGTTCGACGGCCGTTACGTTGCCCGCTTCGCGAGCATTGAGTT
CATTGCCCAGCGCAAGCTCGAGATGCTTGACGACGCCGCCACCCTGGGCGCGTTGCGCTTTCCGCCGGGAAACCGCCTGG
AAAAACTCGGCGGCGATCGCAAGGGACGCTACAGCATCCGCATCAACGGCCAGTGGAGGATCTGCTTCATCTGGACCCAT
GACGGCCCCGCGGCTGTCGAGATCATCGACTATCACTGA

Antitoxin


Download         Length: 110 a.a.        Molecular weight: 12292.12 Da        Isoelectric Point: 5.8834

>AT111922 WP_063240604.1 NZ_CP032518:645023-645352 [Cupriavidus oxalaticus]
MARFENGMRPIHPGEILREEYLVPLNMSANALALALRVTPARISEIVREQRGITPDTALRLTRYFGGDARSWMNMQVTYD
LKVAQRDLGERIAAEVVPRAGIDQEIPES

Download         Length: 330 bp

>AT111922 NZ_CP032518:645023-645352 [Cupriavidus oxalaticus]
ATGGCACGCTTTGAAAACGGCATGCGCCCCATCCATCCGGGCGAAATCCTGCGCGAGGAATACCTGGTGCCGCTGAACAT
GAGCGCCAACGCCCTGGCGCTGGCACTGCGCGTGACGCCGGCCCGCATCAGCGAGATCGTGCGCGAGCAGCGCGGCATCA
CGCCCGACACCGCGCTGCGCCTGACGCGCTATTTTGGTGGCGATGCGCGTTCGTGGATGAATATGCAGGTGACCTACGAC
CTCAAGGTGGCGCAGCGGGACCTGGGCGAGCGCATCGCGGCGGAAGTGGTGCCGCGCGCGGGTATCGACCAGGAAATTCC
GGAAAGCTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A375GMD0

Antitoxin

Source ID Structure
AlphaFold DB A0A375GIG3

References