Detailed information of TA system TA111912

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): prlF-yhaV (relBE)/YhaV-PrlF
• Location: 3752832..3753631
• Replicon: chromosome
• Accession: NZ_CP032515
• Organism: Escherichia coli strain 118UI

Toxin (Protein)

• Gene name: yhaV
• Uniprot ID: B7NDB6
• Locus tag: Eco118UI_RS18810
• Protein ID: WP_000347269.1
• Coordinates: 3753167..3753631 (+)
• Length: 155 a.a.

Antitoxin (Protein)

• Gene name: prlF
• Uniprot ID: V0YUB5
• Locus tag: Eco118UI_RS18805
• Protein ID: WP_001309780.1
• Coordinates: 3752832..3753167 (+)
• Length: 112 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
Eco118UI_RS18790	3748617..3749387	-	771	WP_001058227.1	2-dehydro-3-deoxyglucarate aldolase	-
Eco118UI_RS18795	3749403..3750737	-	1335	WP_000599651.1	galactarate/glucarate/glycerate transporter GarP	-
Eco118UI_RS18800	3751112..3752683	+	1572	WP_001273738.1	galactarate dehydratase	-
Eco118UI_RS18805	3752832..3753167	+	336	WP_001309780.1	type II toxin-antitoxin system antitoxin PrlF	Antitoxin
Eco118UI_RS18810	3753167..3753631	+	465	WP_000347269.1	type II toxin-antitoxin system ribonuclease toxin YhaV	Toxin
Eco118UI_RS18815	3753686..3754495	-	810	WP_000072171.1	aga operon transcriptional regulator AgaR	-
Eco118UI_RS18820	3754744..3756024	+	1281	WP_000681943.1	tagatose-bisphosphate aldolase subunit KbaZ	-
Eco118UI_RS18825	3756047..3756520	+	474	WP_001295547.1	PTS N-acetylgalactosamine transporter subunit IIB	-
Eco118UI_RS18830	3756531..3757310	+	780	WP_000406214.1	PTS mannose/fructose/sorbose/N-acetylgalactosamine transporter subunit IIC	-
Eco118UI_RS18835	3757300..3758178	+	879	WP_001295548.1	PTS system mannose/fructose/sorbose family transporter subunit IID	-
Eco118UI_RS18840	3758196..3758630	+	435	WP_000948818.1	PTS sugar transporter subunit IIA	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	3743590..3753631	10041

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin endonuclease, YhaV (12-150)

Antitoxin

• PrlF antitoxin (14-110)

Sequences

Toxin

Protein

• Length: 155 a.a.
• Molecular weight: 17806.22 Da
• Isoelectric Point: 9.6924

>T111912 WP_000347269.1 NZ_CP032515:3753167-3753631 [Escherichia coli]
MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWETLTREAEETH

Nucleotide

• Length: 465 bp

>T111912 NZ_CP032515:3753167-3753631 [Escherichia coli]
ATGGATTTTCCACAAAGGGTTAATGGTTGGGCGCTATATGCTCATCCCTGTTTTCAGGAAACCTACGACGCTTTAGTTGC
CGAAGTCGAGACATTAAAGGGAAAAGATCCTGAAAATTATCAGAGAAAAGCCGCCACAAAGTTATTGGCGGTAGTCCATA
AAGTGATTGAGGAGCATATCACGGTCAATCCATCATCACCGGCATTCCGTCATGGCAAGTCGTTAGGCTCTGGGAAAAAT
AAAGACTGGTCACGGGTAAAATTTGGTGCTGGTCGTTATCGTCTCTTCTTTCGTTATAGTGAAAAAGAGAAAGTCATCAT
TCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAAACAGATGCTTATACCGTATTCAGCAAAATGT
TAAAAAGAGGACATCCTCCTGCCGACTGGGAAACCCTCACCCGAGAGGCAGAAGAAACCCATTGA

Antitoxin

Protein

• Length: 112 a.a.
• Molecular weight: 12345.94 Da
• Isoelectric Point: 4.8616

>AT111912 WP_001309780.1 NZ_CP032515:3752832-3753167 [Escherichia coli]
MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNT
PQKTRPFNIQQGKKLVAGMDVNIDDEIGDDE

Nucleotide

• Length: 336 bp

>AT111912 NZ_CP032515:3752832-3753167 [Escherichia coli]
ATGCCCGCTAATGCTCGCTCTCACGCTGTACTGACCACTGAATCAAAGGTCACGATACGCGGACAAACAACTATCCCCGC
GCCAGTGCGTGAGGCCTTAAAACTGAAGCCAGGCCAGGACAGCATTCATTACGAAATTCTGCCTGGTGGGCAAGTATTTA
TGTGCCGACTGGGAGATGAACAGGAGGATCATACTATGAATGCATTTTTGCGTTTTCTGGATGCAGATATCCAGAACACC
CCGCAAAAAACTCGTCCATTCAACATTCAACAAGGAAAGAAACTTGTCGCTGGCATGGACGTCAACATTGATGATGAGAT
TGGCGACGACGAATAA

Structures

Toxin

• AlphaFold DB: A0A829IY86

Antitoxin

• AlphaFold DB: V0YUB5