111897

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 1723542..1724104 Replicon chromosome
Accession NZ_CP032515
Organism Escherichia coli strain 118UI

Toxin (Protein)


Gene name higB Uniprot ID L4J948
Locus tag Eco118UI_RS08690 Protein ID WP_000605676.1
Coordinates 1723826..1724104 (-) Length 93 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID S1PAQ1
Locus tag Eco118UI_RS08685 Protein ID WP_000781370.1
Coordinates 1723542..1723826 (-) Length 95 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
Eco118UI_RS08670 1718724..1721771 + 3048 WP_012602782.1 formate dehydrogenase-N subunit alpha -
Eco118UI_RS08675 1721784..1722668 + 885 WP_001240565.1 formate dehydrogenase N subunit beta -
Eco118UI_RS08680 1722661..1723314 + 654 WP_000045646.1 formate dehydrogenase-N subunit gamma -
Eco118UI_RS08685 1723542..1723826 - 285 WP_000781370.1 HigA family addiction module antidote protein Antitoxin
Eco118UI_RS08690 1723826..1724104 - 279 WP_000605676.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
Eco118UI_RS08695 1724290..1725300 - 1011 WP_000642407.1 alcohol dehydrogenase AdhP -
Eco118UI_RS08700 1725434..1727131 - 1698 WP_000433464.1 malate dehydrogenase -
Eco118UI_RS08705 1727288..1727425 - 138 WP_000841554.1 stationary-phase-induced ribosome-associated protein -
Eco118UI_RS08710 1727527..1727742 - 216 WP_000495766.1 biofilm-dependent modulation protein -
Eco118UI_RS08715 1728087..1728518 + 432 WP_000152305.1 peroxiredoxin OsmC -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-1

(4-92)

Antitoxin

Toxin-antitoxin system, antidote protein, HigA

(1-88)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10521.10 Da        Isoelectric Point: 7.3562

>T111897 WP_000605676.1 NZ_CP032515:c1724104-1723826 [Escherichia coli]
MIMNFRHKGLRDLFLLGKTSGVIPTQVKRLRHRLAVIDAASCLADIDMPGYRLHPLSGDRDGIWAISVSGNWRITFEFVN
GDAYILDYEDYH

Download         Length: 279 bp

>T111897 NZ_CP032515:c1724104-1723826 [Escherichia coli]
ATGATAATGAATTTCAGACACAAGGGATTGCGTGACTTGTTTCTTCTCGGCAAAACCTCCGGCGTTATACCGACGCAAGT
CAAACGATTACGCCATCGACTTGCTGTGATTGATGCGGCAAGCTGTCTTGCTGATATCGATATGCCCGGTTACCGACTAC
ACCCGTTAAGCGGCGATCGCGATGGAATTTGGGCGATATCTGTCTCGGGCAACTGGCGAATCACATTTGAATTCGTCAAT
GGCGATGCATACATACTGGATTACGAGGACTATCACTGA

Antitoxin


Download         Length: 95 a.a.        Molecular weight: 10401.08 Da        Isoelectric Point: 8.8567

>AT111897 WP_000781370.1 NZ_CP032515:c1723826-1723542 [Escherichia coli]
MKMANHPRPGDIIQESLDELNVSLREFARAMEIAPSTASRLLTGKAALTPEMAIKLSVVIGSSPQMWLNLQNAWSLAEAE
KTVDVSRLRRLVTQ

Download         Length: 285 bp

>AT111897 NZ_CP032515:c1723826-1723542 [Escherichia coli]
ATGAAAATGGCCAATCATCCCCGCCCGGGGGACATTATTCAGGAATCACTGGACGAACTTAATGTCAGCCTGCGCGAGTT
TGCCAGAGCAATGGAAATTGCGCCCTCAACGGCAAGCCGATTGCTGACCGGAAAAGCAGCTTTGACGCCAGAAATGGCAA
TTAAACTTTCCGTGGTGATCGGCAGTTCGCCGCAAATGTGGCTGAATCTGCAAAACGCCTGGAGTCTGGCAGAGGCTGAA
AAAACGGTGGATGTGTCGCGATTACGCCGTCTGGTTACGCAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829G6T1

Antitoxin

Source ID Structure
AlphaFold DB A0A829CUG6

References