Detailed information of TA system TA111888

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 313272..313922
• Replicon: chromosome
• Accession: NZ_CP032515
• Organism: Escherichia coli strain 118UI

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: Eco118UI_RS01445
• Protein ID: WP_000263532.1
• Coordinates: 313272..313613 (+)
• Length: 114 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: U9YXQ7
• Locus tag: Eco118UI_RS01450
• Protein ID: WP_000212552.1
• Coordinates: 313623..313922 (+)
• Length: 100 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
Eco118UI_RS01420	308307..309407	+	1101	WP_001181876.1	flagellar basal body P-ring protein FlgI	-
Eco118UI_RS01425	309407..309706	+	300	WP_000867281.1	rod-binding protein	-
Eco118UI_RS01430	309882..311258	+	1377	WP_000367245.1	flagellar hook-associated protein FlgK	-
Eco118UI_RS01435	311273..312202	+	930	WP_001266799.1	flagellar hook-associated protein FlgL	-
Eco118UI_RS01440	312219..313196	+	978	WP_001195938.1	hypothetical protein	-
Eco118UI_RS01445	313272..313613	+	342	WP_000263532.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
Eco118UI_RS01450	313623..313922	+	300	WP_000212552.1	helix-turn-helix transcriptional regulator	Antitoxin
Eco118UI_RS01455	313980..314822	-	843	WP_000022366.1	winged helix-turn-helix domain-containing protein	-
Eco118UI_RS01460	315308..316222	+	915	WP_000949083.1	lateral flagellin LafA	-
Eco118UI_RS01470	316427..317743	+	1317	WP_000609678.1	flagellar filament capping protein FliD	-
Eco118UI_RS01475	317766..318158	+	393	WP_000285322.1	flagellar export chaperone FliS	-
Eco118UI_RS01480	318163..318474	+	312	WP_000415004.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (13-107)

Antitoxin

• Cro/C1-type helix-turn-helix domain (31-84)

Sequences

Toxin

Protein

• Length: 114 a.a.
• Molecular weight: 13031.86 Da
• Isoelectric Point: 6.4677

>T111888 WP_000263532.1 NZ_CP032515:313272-313613 [Escherichia coli]
MWDVETTETFDNWFDAQTVALKEDLLAAMLILAEYGPQLGRPFADTVNDSQFSNMKELRVQHQGNPVRAFFAFDPARRGI
VLCAGDKTGLNEKKFYRDMIKLADSEYRKHLKK

Nucleotide

• Length: 342 bp

>T111888 NZ_CP032515:313272-313613 [Escherichia coli]
ATGTGGGACGTCGAGACAACGGAGACTTTCGACAACTGGTTTGATGCCCAGACTGTTGCGCTGAAAGAAGATTTACTTGC
TGCAATGCTGATTCTTGCTGAGTATGGCCCACAGTTGGGCAGACCCTTTGCGGATACGGTTAATGATTCACAGTTTTCAA
ATATGAAAGAATTACGCGTCCAACATCAGGGAAACCCGGTACGTGCTTTTTTTGCTTTCGATCCTGCGCGCCGTGGCATT
GTATTATGTGCGGGTGATAAAACGGGGTTAAATGAGAAAAAGTTTTATCGCGATATGATCAAGCTCGCTGATTCTGAATA
CCGTAAGCATCTGAAAAAGTAA

Antitoxin

Protein

• Length: 100 a.a.
• Molecular weight: 11004.86 Da
• Isoelectric Point: 9.7665

>AT111888 WP_000212552.1 NZ_CP032515:313623-313922 [Escherichia coli]
MATLKELMAKQSPESQVRIAAKVEEMRQTIALHQLREELNISQTEMAAAMGIKQPTLAKMEQADNDPRLSTLKRYVAALG
GELSIDVKLPNGKRIAFQI

Nucleotide

• Length: 300 bp

>AT111888 NZ_CP032515:313623-313922 [Escherichia coli]
ATGGCTACTCTAAAAGAACTGATGGCAAAGCAAAGCCCTGAAAGCCAGGTGCGTATTGCGGCAAAAGTTGAGGAAATGCG
CCAGACCATCGCCTTGCATCAGCTGAGAGAAGAACTCAATATTTCGCAAACGGAAATGGCGGCAGCGATGGGCATTAAGC
AACCGACTCTTGCAAAAATGGAGCAGGCAGATAACGATCCTCGGTTGTCTACGCTAAAACGCTATGTTGCGGCTTTAGGT
GGGGAATTGAGTATCGACGTTAAACTGCCGAATGGTAAGAGAATTGCATTTCAGATTTAA

Structures

Antitoxin

• AlphaFold DB: U9YXQ7