Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type I Classification (family/domain) hok-sok/-
Location 952559..952784 Replicon chromosome
Accession NZ_CP032513
Organism Shigella flexneri strain SFL1520

Toxin (Protein)


Gene name hokW Uniprot ID S1ELB5
Locus tag D6T62_RS04945 Protein ID WP_000813254.1
Coordinates 952559..952714 (-) Length 52 a.a.

Antitoxin (RNA)


Gene name sokW
Locus tag -
Coordinates 952726..952784 (+)

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
D6T62_RS04880 947846..948193 - 348 WP_000631709.1 IS66 family insertion sequence element accessory protein TnpB -
D6T62_RS04885 948190..948864 - 675 WP_004967157.1 IS66-like element accessory protein TnpA -
D6T62_RS04890 948963..949178 - 216 WP_000839572.1 class II holin family protein -
D6T62_RS04920 949974..950690 - 717 Protein_948 bacteriophage antitermination protein Q -
D6T62_RS04925 950687..951052 - 366 WP_000140020.1 RusA family crossover junction endodeoxyribonuclease -
D6T62_RS04930 951053..952111 - 1059 Protein_950 DUF968 domain-containing protein -
D6T62_RS04935 952113..952391 - 279 WP_024260631.1 hypothetical protein -
D6T62_RS04945 952559..952714 - 156 WP_000813254.1 type I toxin-antitoxin system toxin HokD Toxin
- 952726..952784 + 59 - - Antitoxin
D6T62_RS04960 953366..953782 - 417 WP_005069274.1 hypothetical protein -
D6T62_RS04965 953853..954554 + 702 Protein_954 IS1 family transposase -
D6T62_RS04970 954584..954898 + 315 WP_001317924.1 3'-5' exonuclease -
D6T62_RS04975 954957..955160 + 204 WP_000096344.1 DUF4224 domain-containing protein -
D6T62_RS04980 955160..956210 + 1051 Protein_957 tyrosine-type recombinase/integrase -
D6T62_RS04990 956421..957218 + 798 WP_001325918.1 DgsA anti-repressor MtfA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Hok/gef cell toxic protein

(6-47)


Sequences

Toxin        


Download         Length: 52 a.a.        Molecular weight: 5736.89 Da        Isoelectric Point: 6.1531

>T111868 WP_000813254.1 NZ_CP032513:c952714-952559 [Shigella flexneri]
MKQQKAMLIALIVICLTVIVTALVTRKDLCEVRIRTGQTEVAVFTAYEPEE

Download         Length: 156 bp

>T111868 NZ_CP032513:c952714-952559 [Shigella flexneri]
ATGAAGCAGCAAAAGGCGATGTTAATCGCCCTTATCGTCATCTGTTTAACCGTCATAGTGACGGCACTGGTAACGAGGAA
AGACCTCTGCGAGGTACGAATCCGAACCGGCCAGACGGAGGTCGCTGTCTTCACAGCTTACGAACCTGAGGAGTAA

Antitoxin


Download         Length: 59 bp

>AT111868 NZ_CP032513:952726-952784 [Shigella flexneri]
CCTTGCCTTTCGGCACGTAAGAGGCTAACCTACATGTGTTCAGCATGGATTGAGCCTCA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A4P7TMW0

Antitoxin

Download structure file

References