Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 424997..425615 Replicon chromosome
Accession NZ_CP032513
Organism Shigella flexneri strain SFL1520

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag D6T62_RS02210 Protein ID WP_001291435.1
Coordinates 424997..425215 (-) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID Q0T7C3
Locus tag D6T62_RS02215 Protein ID WP_000344797.1
Coordinates 425241..425615 (-) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
D6T62_RS02180 (420889) 420889..421200 - 312 WP_000409911.1 MGMT family protein -
D6T62_RS02190 (421579) 421579..421932 + 354 WP_000878140.1 DUF1428 domain-containing protein -
D6T62_RS02195 (421974) 421974..423524 - 1551 WP_005066536.1 cyclic-guanylate-specific phosphodiesterase PdeB -
D6T62_RS02200 (423688) 423688..424158 - 471 WP_000136192.1 YlaC family protein -
D6T62_RS02205 (424274) 424274..424825 - 552 WP_000102569.1 maltose O-acetyltransferase -
D6T62_RS02210 (424997) 424997..425215 - 219 WP_001291435.1 HHA domain-containing protein Toxin
D6T62_RS02215 (425241) 425241..425615 - 375 WP_000344797.1 Hha toxicity modulator TomB Antitoxin
D6T62_RS02220 (426161) 426161..429310 - 3150 WP_001132472.1 efflux RND transporter permease AcrB -
D6T62_RS02225 (429333) 429333..430526 - 1194 WP_024260158.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
flank IS/Tn - - 419531..420859 1328


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T111866 WP_001291435.1 NZ_CP032513:c425215-424997 [Shigella flexneri]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T111866 NZ_CP032513:c425215-424997 [Shigella flexneri]
ATGTCCGAAAAACCTTTAACAAAAACCGATTATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTGTACGACAAGATCCCTTCCTCAGTATGGAAATTTATTCGCTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14513.38 Da        Isoelectric Point: 4.9091

>AT111866 WP_000344797.1 NZ_CP032513:c425615-425241 [Shigella flexneri]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQALQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT111866 NZ_CP032513:c425615-425241 [Shigella flexneri]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACCCTGTATCATGACTGTCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTTAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGCAGTTATGGTATTAATATGCAGGCTCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A4P7TPD8

References