Detailed information of TA system TA111827

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): DinJ-YafQ (relBE)/YafQ(toxin)
• Location: 1562520..1563108
• Replicon: chromosome
• Accession: NZ_CP032479
• Organism: Helicobacter pylori strain 21-F-EK1

Toxin (Protein)

• Gene name: TfiT
• Uniprot ID: A0A496ESF5
• Locus tag: D4I21_RS07685
• Protein ID: WP_033756152.1
• Coordinates: 1562827..1563108 (+)
• Length: 94 a.a.

Antitoxin (Protein)

• Gene name: TfiA
• Uniprot ID: -
• Locus tag: D4I21_RS07680
• Protein ID: WP_139543530.1
• Coordinates: 1562520..1562834 (+)
• Length: 105 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
D4I21_RS07660	1558056..1558721	+	666	WP_139543527.1	ParA family protein	-
D4I21_RS07665	1558795..1559079	+	285	WP_000394655.1	hypothetical protein	-
D4I21_RS07670	1559111..1560289	+	1179	WP_139543528.1	hypothetical protein	-
D4I21_RS07675	1560314..1562227	-	1914	WP_139543529.1	relaxase/mobilization nuclease domain-containing protein	-
D4I21_RS07680	1562520..1562834	+	315	WP_139543530.1	hypothetical protein	Antitoxin
D4I21_RS07685	1562827..1563108	+	282	WP_033756152.1	type II toxin-antitoxin system mRNA interferase toxin, RelE/StbE family	Toxin
D4I21_RS07690	1563383..1565110	-	1728	WP_139543531.1	type IV secretory system conjugative DNA transfer family protein	-
D4I21_RS07695	1565111..1565668	-	558	WP_139543532.1	hypothetical protein	-
D4I21_RS07700	1565669..1565959	-	291	WP_000159818.1	hypothetical protein	-
D4I21_RS07705	1565956..1566411	-	456	WP_139543533.1	mobilization protein	-
D4I21_RS07710	1566408..1567349	-	942	WP_139543534.1	Flp pilus assembly complex ATPase component TadA	-
D4I21_RS07715	1567349..1567648	-	300	WP_000660141.1	hypothetical protein	-
D4I21_RS07720	1567645..1567908	-	264	WP_139543535.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, YafQ-like toxin (1-92)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 94 a.a.
• Molecular weight: 10874.86 Da
• Isoelectric Point: 10.0264

>T111827 WP_033756152.1 NZ_CP032479:1562827-1563108 [Helicobacter pylori]
MLKVRTKKDFLKDFNKHILSGRITESDVASIVDCLKKQKPLQQKYCDHALSGNLKGLRECHVKPNLLLIYEIKKQENELV
LLRLDTHSELFKK

Nucleotide

• Length: 282 bp

>T111827 NZ_CP032479:1562827-1563108 [Helicobacter pylori]
ATGCTTAAAGTCAGAACTAAGAAAGATTTTCTTAAAGACTTTAATAAGCATATCTTATCTGGGCGTATTACAGAAAGCGA
TGTTGCAAGCATTGTTGATTGCCTAAAAAAACAAAAACCACTCCAACAAAAATATTGCGACCATGCCTTGAGTGGCAATC
TTAAAGGGTTGAGAGAGTGTCATGTCAAGCCAAACTTGTTATTGATTTATGAAATCAAAAAACAAGAAAACGAACTTGTT
TTATTGCGTCTAGACACTCATAGCGAGCTATTTAAAAAGTGA

Antitoxin

Protein

• Length: 105 a.a.
• Molecular weight: 12149.66 Da
• Isoelectric Point: 6.2317

>AT111827 WP_139543530.1 NZ_CP032479:1562520-1562834 [Helicobacter pylori]
MSNIITDYSQYNEKQLHNFLNAIKKQLLKAEGDKNKAIKKIQECELQEQMIRQVLAQKHSQEKEPTPSLLNTIASKDDPE
YDVSFGDFNDFLQIAKQERERHNA

Nucleotide

• Length: 315 bp

>AT111827 NZ_CP032479:1562520-1562834 [Helicobacter pylori]
ATGTCAAATATTATTACAGATTATTCGCAATACAATGAAAAACAACTGCATAATTTCTTAAACGCCATTAAAAAACAATT
GCTTAAGGCAGAAGGGGATAAAAATAAAGCGATAAAAAAAATCCAAGAGTGTGAATTGCAAGAACAAATGATCAGACAAG
TTTTAGCCCAAAAACATTCTCAAGAAAAAGAACCCACACCAAGTTTGTTAAATACGATCGCCTCAAAAGATGACCCAGAA
TACGATGTTTCGTTTGGAGACTTCAATGATTTTTTGCAAATAGCAAAACAAGAAAGAGAAAGGCATAATGCTTAA

Structures

Toxin

• AlphaFold DB: A0A496ESF5