Detailed information of TA system TA111813

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): DinJ-YafQ (relBE)/YafQ(toxin)
• Location: 1631050..1631638
• Replicon: chromosome
• Accession: NZ_CP032471
• Organism: Helicobacter pylori strain 479-C2-EK2

Toxin (Protein)

• Gene name: TfiT
• Uniprot ID: K2LF18
• Locus tag: D4L66_RS07925
• Protein ID: WP_000921926.1
• Coordinates: 1631357..1631638 (+)
• Length: 94 a.a.

Antitoxin (Protein)

• Gene name: TfiA
• Uniprot ID: -
• Locus tag: D4L66_RS07920
• Protein ID: WP_139519002.1
• Coordinates: 1631050..1631364 (+)
• Length: 105 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
D4L66_RS07900	1626572..1627228	+	657	WP_000587377.1	ParA family protein	-
D4L66_RS07905	1627309..1627593	+	285	WP_079296630.1	hypothetical protein	-
D4L66_RS07910	1627637..1628821	+	1185	WP_139519000.1	hypothetical protein	-
D4L66_RS07915	1628846..1630756	-	1911	WP_139519001.1	relaxase/mobilization nuclease domain-containing protein	-
D4L66_RS07920	1631050..1631364	+	315	WP_139519002.1	hypothetical protein	Antitoxin
D4L66_RS07925	1631357..1631638	+	282	WP_000921926.1	type II toxin-antitoxin system mRNA interferase toxin, RelE/StbE family	Toxin
D4L66_RS07930	1631913..1633640	-	1728	WP_180339203.1	type IV secretory system conjugative DNA transfer family protein	-
D4L66_RS07935	1633641..1634198	-	558	WP_139519003.1	hypothetical protein	-
D4L66_RS07940	1634365..1634991	-	627	WP_000451922.1	FAD-dependent thymidylate synthase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	flgG / pdxA / pdxJ / flhB2 / flgB / flgC / fliE	1517875..1635593	117718

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, YafQ-like toxin (1-92)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 94 a.a.
• Molecular weight: 10932.89 Da
• Isoelectric Point: 9.8257

>T111813 WP_000921926.1 NZ_CP032471:1631357-1631638 [Helicobacter pylori]
MLKVRTKKDFLKDFNKHILSGRIKESDVTSIVDCLKEQKPLQQKYCDHALSGNLKGLRECHVKPNLLLIYEIKKQENELV
LLRLDTHSELFKK

Nucleotide

• Length: 282 bp

>T111813 NZ_CP032471:1631357-1631638 [Helicobacter pylori]
ATGCTTAAAGTCAGAACTAAGAAAGATTTTCTTAAAGACTTTAATAAGCATATCTTATCCGGGCGTATTAAAGAAAGCGA
TGTTACAAGCATTGTTGATTGCCTCAAAGAGCAAAAGCCACTCCAACAAAAATATTGCGACCATGCCTTGAGTGGCAATC
TTAAAGGGCTGAGAGAGTGTCATGTCAAGCCAAACTTGTTATTGATTTATGAAATCAAAAAACAAGAAAACGAACTTGTT
TTATTGCGTCTAGACACTCATAGCGAGCTATTTAAAAAGTGA

Antitoxin

Protein

• Length: 105 a.a.
• Molecular weight: 12122.56 Da
• Isoelectric Point: 4.9439

>AT111813 WP_139519002.1 NZ_CP032471:1631050-1631364 [Helicobacter pylori]
MSNIITDYSQYNEKQLHNFLNAIEKQLLKAEGDKNKAIKKIQECELQEQMIRQVLAQKHSQEKEPTQSLLNTIASKDDPE
CDVSFGDFDDFLQIAKQERERHNA

Nucleotide

• Length: 315 bp

>AT111813 NZ_CP032471:1631050-1631364 [Helicobacter pylori]
ATGTCAAATATTATTACAGATTACTCGCAATACAATGAAAAGCAACTGCACAATTTCTTAAACGCCATTGAAAAACAATT
GCTTAAGGCAGAAGGGGATAAAAACAAGGCGATAAAAAAAATCCAAGAGTGTGAATTGCAAGAACAAATGATCAGACAAG
TTTTAGCCCAAAAACATTCTCAAGAAAAAGAACCCACACAAAGTTTGTTAAATACGATCGCCTCAAAAGATGACCCAGAA
TGCGATGTTTCGTTTGGAGACTTTGATGATTTTTTACAAATAGCAAAACAAGAAAGAGAAAGGCATAATGCTTAA

Structures

Toxin

• AlphaFold DB: K2LF18