Detailed information of TA system TA111767

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 43449..43703
• Replicon: plasmid pOXA1_020001
• Accession: NZ_CP032425
• Organism: Escherichia coli strain SCEC020001

Toxin (Protein)

• Gene name: srnB
• Uniprot ID: -
• Locus tag: C7V21_RS00655
• Protein ID: WP_015387399.1
• Coordinates: 43449..43655 (-)
• Length: 69 a.a.

Antitoxin (RNA)

Gene name	srnC
Locus tag	-
Coordinates	43642..43703 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C7V21_RS00620	38696..39607	-	912	WP_000440183.1	carbamate kinase	-
C7V21_RS00625	39618..40838	-	1221	WP_000410951.1	arginine deiminase	-
C7V21_RS00630	41731..42213	+	483	Protein_47	VENN motif pre-toxin domain-containing protein	-
C7V21_RS00635	42159..42605	-	447	Protein_48	incFII family plasmid replication initiator RepA	-
C7V21_RS00640	42598..42672	-	75	WP_032336874.1	RepA leader peptide Tap	-
C7V21_RS00650	42908..43165	-	258	WP_000083833.1	replication regulatory protein RepA	-
C7V21_RS00655	43449..43655	-	207	WP_015387399.1	type I toxin-antitoxin system Hok family toxin	Toxin
-	43642..43703	+	62	NuclAT_0	-	Antitoxin
-	43642..43703	+	62	NuclAT_0	-	Antitoxin
-	43642..43703	+	62	NuclAT_0	-	Antitoxin
-	43642..43703	+	62	NuclAT_0	-	Antitoxin
C7V21_RS00665	43842..44024	-	183	WP_000968309.1	hypothetical protein	-
C7V21_RS00670	44125..44741	+	617	Protein_53	IS1 family transposase	-
C7V21_RS00675	44779..46350	-	1572	WP_023149734.1	IS66 family transposase	-
C7V21_RS00680	46370..46717	-	348	WP_000624622.1	IS66 family insertion sequence element accessory protein TnpB	-
C7V21_RS00685	46717..47394	-	678	WP_001339397.1	IS66 family insertion sequence hypothetical protein	-
C7V21_RS25645	47449..47538	+	90	Protein_57	IS1 family transposase	-
C7V21_RS00700	47839..48051	-	213	WP_005012601.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	blaOXA-1 / aac(6')-Ib-cr / tet(A) / sitABCD	iutA / iucD / iucC / iucB / iucA	1..88392	88392

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (23-64)

Sequences

Toxin

Protein

• Length: 69 a.a.
• Molecular weight: 7720.23 Da
• Isoelectric Point: 9.3127

>T111767 WP_015387399.1 NZ_CP032425:c43655-43449 [Escherichia coli]
MKYLNTTDCSLFLAGRSKFMTKYALIGLLAVCATVLCFSLIFRERLCELNIHRGNTVVQVTLAYEARK

Nucleotide

• Length: 207 bp

>T111767 NZ_CP032425:c43655-43449 [Escherichia coli]
ATGAAGTACCTTAACACTACTGATTGTAGCCTCTTCCTTGCAGGGAGGTCAAAGTTTATGACGAAATATGCCCTTATCGG
GTTGCTCGCCGTGTGCGCCACGGTGTTGTGTTTTTCACTGATATTCAGGGAACGGTTATGTGAACTGAATATTCACAGGG
GAAATACAGTGGTGCAGGTAACTCTGGCCTACGAAGCACGGAAGTAA

Antitoxin

Nucleotide

• Length: 62 bp

>AT111767 NZ_CP032425:43642-43703 [Escherichia coli]
TTAAGGTACTTCATGCAGACCTCACGAGTTAATGGATTAACGAGTGGGGTCTTCGCATTTCT

Structures

Antitoxin