Detailed information of TA system TA111700

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 138838..139484
• Replicon: plasmid p15WZ-82_res
• Accession: NZ_CP032357
• Organism: Klebsiella variicola strain 15WZ-82

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: D4N21_RS30150
• Protein ID: WP_114435893.1
• Coordinates: 139251..139484 (-)
• Length: 78 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: D4N21_RS30145
• Protein ID: WP_049010942.1
• Coordinates: 138838..139254 (-)
• Length: 139 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
D4N21_RS30110	134455..134934	+	480	WP_063106392.1	transglycosylase SLT domain-containing protein	-
D4N21_RS30115	134972..135502	-	531	WP_128206247.1	antirestriction protein	-
D4N21_RS30120	136127..136960	-	834	WP_128206267.1	N-6 DNA methylase	-
D4N21_RS30125	137007..137237	-	231	WP_004197732.1	hypothetical protein	-
D4N21_RS30130	137325..137672	-	348	WP_114435895.1	hypothetical protein	-
D4N21_RS30135	137738..138118	-	381	WP_114268482.1	hypothetical protein	-
D4N21_RS30145	138838..139254	-	417	WP_049010942.1	helix-turn-helix domain-containing protein	Antitoxin
D4N21_RS30150	139251..139484	-	234	WP_114435893.1	type II toxin-antitoxin system HigB family toxin	Toxin
D4N21_RS30155	139635..140981	+	1347	WP_128206248.1	site-specific integrase	-
D4N21_RS30160	140869..141814	+	946	Protein_147	DUF1016 family protein	-
D4N21_RS31280	141806..142390	+	585	Protein_148	DUF945 domain-containing protein	-
D4N21_RS30165	142470..142763	-	294	WP_114435891.1	helix-turn-helix domain-containing protein	-
D4N21_RS30170	143050..143385	+	336	WP_114435890.1	DUF2958 domain-containing protein	-
D4N21_RS30175	143732..144334	+	603	Protein_151	DUF2285 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	-	1..185478	185478

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, mRNA interferase HigB (6-67)

Antitoxin

• Cro/C1-type helix-turn-helix domain (84-133)

Sequences

Toxin

Protein

• Length: 78 a.a.
• Molecular weight: 9641.08 Da
• Isoelectric Point: 10.2579

>T111700 WP_114435893.1 NZ_CP032357:c139484-139251 [Klebsiella variicola]
IPGWSHRQTKKPDELKRYFTSLDRMKYREKWWVIDIGGNNLRMMFFADFERGKIFVKHITTHAEYDRLTKYYREHKE

Nucleotide

• Length: 234 bp

>T111700 NZ_CP032357:c139484-139251 [Klebsiella variicola]
ATACCGGGCTGGAGTCATCGTCAAACAAAAAAACCGGATGAGCTAAAGCGATACTTTACCAGTCTGGATCGCATGAAATA
CCGGGAAAAATGGTGGGTTATCGATATCGGTGGTAATAACCTTAGAATGATGTTTTTTGCTGATTTTGAGCGCGGAAAGA
TATTCGTCAAACACATCACCACCCATGCTGAATATGATCGCTTAACGAAATATTATCGGGAGCACAAAGAATGA

Antitoxin

Protein

• Length: 139 a.a.
• Molecular weight: 15438.52 Da
• Isoelectric Point: 4.4711

>AT111700 WP_049010942.1 NZ_CP032357:c139254-138838 [Klebsiella variicola]
MIFSDAIKAANDLASIVPLLGGSSSRKDYEEALKLVEYLLEHDPDSPLVDMLTARIDAWEDNAVEFEEFNTRIEAGKNGV
SLLRVLMQQHGLSQSDFENEIGKKSLVSRILSGERSLTLDHMRALANRFQIPVSMFVD

Nucleotide

• Length: 417 bp

>AT111700 NZ_CP032357:c139254-138838 [Klebsiella variicola]
ATGATTTTTAGCGATGCCATTAAAGCGGCGAATGACCTGGCGAGTATTGTCCCTCTGCTGGGTGGCAGCTCGTCCCGCAA
AGATTACGAAGAGGCGCTTAAACTCGTTGAATATCTTCTGGAACATGACCCGGACAGCCCGCTGGTTGATATGCTGACTG
CCCGCATTGATGCCTGGGAAGATAACGCGGTGGAATTTGAAGAATTTAATACGCGCATCGAAGCAGGCAAGAACGGTGTT
TCGTTGCTGCGGGTTCTCATGCAGCAACACGGGCTTTCACAGTCCGATTTTGAAAACGAGATAGGCAAAAAATCGCTCGT
CAGCCGGATCCTCAGTGGTGAACGCAGTCTGACCCTCGATCACATGCGGGCGCTGGCAAATCGATTTCAAATCCCGGTCT
CGATGTTTGTTGACTGA