Detailed information of TA system TA111699

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/RelE-HTH
• Location: 73849..74523
• Replicon: plasmid p15WZ-82_res
• Accession: NZ_CP032357
• Organism: Klebsiella variicola strain 15WZ-82

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: D4N21_RS29735
• Protein ID: WP_012539985.1
• Coordinates: 74200..74523 (-)
• Length: 108 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: D4N21_RS29730
• Protein ID: WP_012540018.1
• Coordinates: 73849..74151 (-)
• Length: 101 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
D4N21_RS29695	69555..70076	+	522	WP_101880787.1	hypothetical protein	-
D4N21_RS29700	70178..70600	-	423	Protein_63	SOS response-associated peptidase family protein	-
D4N21_RS29705	70997..71416	+	420	WP_012540023.1	ASCH domain-containing protein	-
D4N21_RS29710	71945..72106	+	162	Protein_65	transposase	-
D4N21_RS29715	72332..72667	+	336	WP_032700858.1	TonB family protein	-
D4N21_RS29720	72692..73234	+	543	WP_012539969.1	GNAT family N-acetyltransferase	-
D4N21_RS29725	73523..73813	+	291	WP_012540036.1	hypothetical protein	-
D4N21_RS29730	73849..74151	-	303	WP_012540018.1	helix-turn-helix domain-containing protein	Antitoxin
D4N21_RS29735	74200..74523	-	324	WP_012539985.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
D4N21_RS29740	74622..75299	+	678	Protein_71	IS110 family transposase	-
D4N21_RS29745	75436..76062	-	627	WP_170873325.1	peptidase	-
D4N21_RS29755	76565..77560	+	996	WP_012539965.1	IS110 family transposase	-
D4N21_RS29760	77681..78475	-	795	WP_012540031.1	helix-turn-helix transcriptional regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	-	1..185478	185478
	-	flank	IS/Tn	-	-	71945..72118	173

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-2 (45-106)

Antitoxin

• Cro/C1-type helix-turn-helix domain (39-83)

Sequences

Toxin

Protein

• Length: 108 a.a.
• Molecular weight: 12358.27 Da
• Isoelectric Point: 9.6240

>T111699 WP_012539985.1 NZ_CP032357:c74523-74200 [Klebsiella variicola]
MNYLEFVETSAFSGLRKELMDDDEFRELQTYLLEAHDRGDTISHTGGCRKIRWGRPGMGKRGGVRVIYYVRLASGRLYLL
LIYPKNAKDDLSEKEKAVMKALTQQLK

Nucleotide

• Length: 324 bp

>T111699 NZ_CP032357:c74523-74200 [Klebsiella variicola]
ATGAACTATCTGGAGTTTGTTGAAACCAGCGCTTTCAGTGGACTGCGTAAGGAGCTCATGGATGATGATGAATTCCGCGA
ACTGCAGACCTATTTACTGGAAGCCCATGATCGGGGTGACACAATCAGTCATACCGGTGGATGCAGGAAGATTCGCTGGG
GGCGCCCGGGGATGGGTAAGCGAGGTGGGGTCAGGGTGATTTACTACGTTCGTCTTGCCAGCGGCAGACTGTATCTGCTG
CTGATATACCCGAAGAATGCGAAAGATGATCTCAGCGAGAAAGAAAAAGCCGTTATGAAGGCATTAACACAGCAGCTGAA
GTAA

Antitoxin

Protein

• Length: 101 a.a.
• Molecular weight: 11226.13 Da
• Isoelectric Point: 6.8718

>AT111699 WP_012540018.1 NZ_CP032357:c74151-73849 [Klebsiella variicola]
MEKKLFDALISSMEEMVAIENGQIQPAAEQVHRHTLPDVKAIRKHAGMKQAEFAEVVGASVDLVRSWEQQRRIPSGIALK
MLCLIEREPALIDTLRGIHV

Nucleotide

• Length: 303 bp

>AT111699 NZ_CP032357:c74151-73849 [Klebsiella variicola]
ATGGAAAAGAAACTTTTTGATGCCCTGATCAGCAGCATGGAGGAAATGGTGGCCATTGAAAATGGCCAGATCCAGCCCGC
TGCAGAACAGGTTCACCGCCATACACTGCCGGATGTGAAAGCCATTCGTAAACATGCCGGCATGAAACAGGCTGAATTTG
CAGAAGTGGTGGGTGCCAGCGTTGACCTTGTCCGCAGCTGGGAACAGCAACGGCGTATTCCGTCGGGTATAGCGCTGAAA
ATGCTCTGCCTCATCGAGCGTGAGCCTGCACTGATTGATACGTTGCGTGGTATTCACGTCTGA