Detailed information of TA system TA111693

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/Tad-HTH_37
• Location: 4469829..4470426
• Replicon: chromosome
• Accession: NZ_CP032354
• Organism: Klebsiella variicola strain 15WZ-82

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A0B7GEF2
• Locus tag: D4N21_RS22240
• Protein ID: WP_012542526.1
• Coordinates: 4470109..4470426 (-)
• Length: 106 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: D4N21_RS22235
• Protein ID: WP_012542525.1
• Coordinates: 4469829..4470116 (-)
• Length: 96 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
D4N21_RS22205	4465739..4465987	+	249	WP_008805539.1	DUF1158 domain-containing protein	-
D4N21_RS22210	4466004..4466345	-	342	WP_002893035.1	RamA family antibiotic efflux transcriptional regulator	-
D4N21_RS22215	4466376..4467491	-	1116	WP_162493283.1	MBL fold metallo-hydrolase	-
D4N21_RS22220	4467671..4468252	+	582	WP_012968754.1	TetR/AcrR family transcriptional regulator	-
D4N21_RS22225	4468252..4468620	+	369	WP_008805536.1	MmcQ/YjbR family DNA-binding protein	-
D4N21_RS22230	4468740..4469393	+	654	WP_012968755.1	oxygen-insensitive NAD(P)H nitroreductase	-
D4N21_RS22235	4469829..4470116	-	288	WP_012542525.1	helix-turn-helix transcriptional regulator	Antitoxin
D4N21_RS22240	4470109..4470426	-	318	WP_012542526.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
D4N21_RS22245	4470611..4471654	-	1044	WP_016160437.1	DUF2157 domain-containing protein	-
D4N21_RS22255	4472184..4473050	-	867	WP_008805530.1	helix-turn-helix transcriptional regulator	-
D4N21_RS22260	4473159..4474586	+	1428	WP_101837261.1	MFS transporter	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (12-100)

Antitoxin

• Cro/C1-type helix-turn-helix domain (38-89)

Sequences

Toxin

Protein

• Length: 106 a.a.
• Molecular weight: 12110.39 Da
• Isoelectric Point: 11.2767

>T111693 WP_012542526.1 NZ_CP032354:c4470426-4470109 [Klebsiella variicola]
MFRLVVHVDVKKELQALPPIVQAKMIRQIDKLRQNPTALREPDSKPLGQGLFEIRALGSVQGRGIYVFQQGKTLFLLRVF
VKKTQKTPSSEIRLALKRLEEMRNE

Nucleotide

• Length: 318 bp

>T111693 NZ_CP032354:c4470426-4470109 [Klebsiella variicola]
ATGTTCAGATTGGTTGTGCATGTGGACGTTAAAAAGGAATTGCAGGCGCTTCCGCCCATTGTCCAGGCGAAGATGATCCG
GCAAATAGACAAGTTACGCCAAAATCCCACCGCGCTGAGAGAACCTGACAGTAAACCGCTGGGGCAGGGGCTATTTGAAA
TTCGTGCGTTAGGTTCGGTACAGGGGCGAGGGATCTACGTTTTTCAGCAAGGCAAGACGCTCTTTCTGCTACGGGTTTTT
GTCAAGAAAACGCAAAAAACCCCGTCATCGGAAATTCGCCTGGCGCTGAAGCGATTAGAGGAGATGCGCAATGAGTAA

Antitoxin

Protein

• Length: 96 a.a.
• Molecular weight: 10815.51 Da
• Isoelectric Point: 10.2212

>AT111693 WP_012542525.1 NZ_CP032354:c4470116-4469829 [Klebsiella variicola]
MSKKIIDWDTLKTELLSQPEVQAAFDAQERQQRLRTMLAQWREQAGLTRAQVAQRMGVSAPTVSRMEANITRASLDTLTR
YALACGIKHPQILLY

Nucleotide

• Length: 288 bp

>AT111693 NZ_CP032354:c4470116-4469829 [Klebsiella variicola]
ATGAGTAAAAAGATCATTGACTGGGATACGTTAAAAACAGAACTGCTTAGCCAGCCTGAGGTTCAGGCTGCATTCGATGC
GCAAGAACGTCAACAGCGATTACGTACTATGCTGGCGCAATGGCGCGAGCAGGCGGGGCTGACCCGAGCCCAGGTGGCGC
AGAGGATGGGGGTGAGTGCGCCGACGGTCTCCAGAATGGAAGCCAATATCACCCGCGCCAGTCTGGATACCTTAACGCGA
TACGCGTTAGCCTGTGGAATAAAGCACCCACAAATTTTACTTTACTAA

Structures

Toxin

• AlphaFold DB: A0A0B7GEF2