Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/-
Location 4491947..4492601 Replicon chromosome
Accession NZ_CP032353
Organism Pseudomonas protegens strain pf5-k2

Toxin (Protein)


Gene name higB Uniprot ID Q4K9S3
Locus tag D4N37_RS20305 Protein ID WP_011062197.1
Coordinates 4491947..4492297 (+) Length 117 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID Q4K9S2
Locus tag D4N37_RS20310 Protein ID WP_011062198.1
Coordinates 4492284..4492601 (+) Length 106 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
D4N37_RS20295 4488706..4490238 + 1533 WP_011062195.1 NADH-quinone oxidoreductase subunit M -
D4N37_RS20300 4490246..4491709 + 1464 WP_011062196.1 NADH-quinone oxidoreductase subunit NuoN -
D4N37_RS20305 4491947..4492297 + 351 WP_011062197.1 hypothetical protein Toxin
D4N37_RS20310 4492284..4492601 + 318 WP_011062198.1 transcriptional regulator Antitoxin
D4N37_RS20315 4492670..4494052 - 1383 WP_011062199.1 cysteine--tRNA ligase -
D4N37_RS20320 4494071..4495771 - 1701 WP_011062200.1 glutamine--tRNA ligase/YqeY domain fusion protein -
D4N37_RS20325 4496042..4496545 + 504 WP_011062201.1 peptidyl-prolyl cis-trans isomerase -
D4N37_RS20330 4496542..4497294 + 753 WP_011062202.1 UDP-2,3-diacylglucosamine diphosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Integrative and Conjugative Element - - 4318880..4568435 249555


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin


No domain identified.


Antitoxin

Cro/C1-type helix-turn-helix domain

(48-101)


Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 13922.05 Da        Isoelectric Point: 9.6226

>T111677 WP_011062197.1 NZ_CP032353:4491947-4492297 [Pseudomonas protegens]
MNALFIELPAFARYRKGFLDDELFCELQIELLRHPHAGVLIQGTGGLRKMRFVDERRNKGRRGGLRVIYYWWSGGAQFWL
FTLFDKEQMDDLLPQQRQQLKQLLEREIEARRHDQT

Download         Length: 351 bp

>T111677 NZ_CP032353:4491947-4492297 [Pseudomonas protegens]
ATGAATGCTCTGTTTATTGAACTACCGGCATTTGCGCGATACCGAAAAGGCTTCCTTGACGACGAGTTGTTCTGCGAACT
GCAGATCGAGTTGCTGCGTCACCCGCACGCGGGTGTTCTGATTCAGGGCACTGGCGGCTTGCGCAAGATGCGTTTCGTCG
ATGAGCGACGCAACAAGGGCAGGCGTGGCGGGCTGAGGGTGATTTATTACTGGTGGTCCGGAGGAGCACAGTTCTGGCTC
TTCACCCTGTTCGACAAGGAGCAGATGGACGACCTGCTACCGCAGCAGCGACAACAGCTCAAACAACTGCTGGAACGTGA
AATCGAGGCCAGACGACATGACCAAACGTGA

Antitoxin


Download         Length: 106 a.a.        Molecular weight: 12064.95 Da        Isoelectric Point: 10.4439

>AT111677 WP_011062198.1 NZ_CP032353:4492284-4492601 [Pseudomonas protegens]
MTKRDIFSELVEGIDALTQERQGKITLRSHKVKLEKLPPITAAEVMNIRQQLNLSRSVFAMYLRTNTRTLENWEQGRAIP
NAQATTLIRLVERYPDTVERLASLA

Download         Length: 318 bp

>AT111677 NZ_CP032353:4492284-4492601 [Pseudomonas protegens]
ATGACCAAACGTGACATCTTTTCCGAACTGGTAGAAGGCATCGACGCCCTCACCCAGGAACGCCAGGGCAAGATCACCCT
GCGCAGCCATAAAGTGAAGCTCGAAAAGCTGCCGCCCATCACCGCCGCCGAGGTGATGAACATCCGCCAGCAACTCAACC
TCTCGCGCTCGGTATTCGCCATGTACCTGCGCACCAACACCCGCACCCTGGAGAACTGGGAACAGGGCCGCGCCATACCC
AACGCCCAGGCCACGACCTTGATACGCCTGGTGGAACGCTACCCCGATACAGTGGAGCGCCTCGCCTCGCTGGCCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB Q4K9S3

Antitoxin

Source ID Structure
AlphaFold DB A0A2C9EPZ9

References