Detailed information of TA system TA111665

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 3447478..3448205
• Replicon: chromosome
• Accession: NZ_CP032329
• Organism: Xenorhabdus nematophila strain YL001

Toxin (Protein)

• Gene name: higB
• Uniprot ID: D3VFW9
• Locus tag: D3790_RS15735
• Protein ID: WP_013185754.1
• Coordinates: 3447478..3447789 (+)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: D3790_RS15740
• Protein ID: WP_013185753.1
• Coordinates: 3447786..3448205 (+)
• Length: 140 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
D3790_RS15720	3443243..3443530	-	288	WP_010846183.1	1,2-phenylacetyl-CoA epoxidase subunit B	-
D3790_RS15725	3443601..3444542	-	942	WP_010846184.1	1,2-phenylacetyl-CoA epoxidase subunit A	-
D3790_RS15730	3444871..3446943	+	2073	WP_038219368.1	phenylacetic acid degradation bifunctional protein PaaZ	-
D3790_RS15735	3447478..3447789	+	312	WP_013185754.1	type II toxin-antitoxin system HigB family toxin	Toxin
D3790_RS15740	3447786..3448205	+	420	WP_013185753.1	helix-turn-helix domain-containing protein	Antitoxin
D3790_RS15745	3448305..3448472	+	168	WP_038219367.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	-
D3790_RS15750	3448514..3449638	+	1125	Protein_3001	IS701 family transposase	-
D3790_RS15755	3450027..3450356	+	330	WP_010846191.1	universal stress protein UspB	-
D3790_RS15760	3450482..3451972	-	1491	WP_013185749.1	inorganic phosphate transporter PitA	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, mRNA interferase HigB (21-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (84-138)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12535.52 Da
• Isoelectric Point: 10.0558

>T111665 WP_013185754.1 NZ_CP032329:3447478-3447789 [Xenorhabdus nematophila]
MHVISKEPFEQAVRDYPNDANVLITTYKLLKEKNFDSPLELQKLFPSLDNFKHRNKWWVIDIGGNNLRMIAYINFMNKRF
YVKHIVTHKEYDKLTKKYRETKE

Nucleotide

• Length: 312 bp

>T111665 NZ_CP032329:3447478-3447789 [Xenorhabdus nematophila]
ATGCACGTTATTTCTAAGGAACCCTTTGAGCAAGCTGTTCGTGATTATCCCAATGATGCAAACGTCCTTATCACGACGTA
TAAGCTGTTAAAAGAAAAGAATTTTGATAGCCCGTTGGAATTACAAAAACTGTTTCCGAGTCTTGATAATTTTAAGCACA
GGAACAAATGGTGGGTAATCGACATAGGTGGCAATAACTTACGGATGATTGCCTATATCAACTTTATGAACAAACGCTTT
TACGTTAAGCACATCGTCACTCACAAAGAATATGACAAATTGACAAAGAAGTATCGGGAGACAAAAGAATGA

Antitoxin

Protein

• Length: 140 a.a.
• Molecular weight: 15251.61 Da
• Isoelectric Point: 5.6660

>AT111665 WP_013185753.1 NZ_CP032329:3447786-3448205 [Xenorhabdus nematophila]
MIREPIKALQATTALIAAVPFLGSSASEKDYRDALAMVDYLIENHDESPLINILAAKIAEYEDSNKRFAAFNKAVEVMPT
GVAALRVLMDQHGLSYSDLQEEIGSKSLISQIFSGKRALTIAHIKALSARFDVKPDIFL

Nucleotide

• Length: 420 bp

>AT111665 NZ_CP032329:3447786-3448205 [Xenorhabdus nematophila]
ATGATAAGAGAACCTATCAAAGCCCTTCAAGCGACCACGGCTCTGATCGCTGCTGTTCCCTTTTTAGGGAGCAGTGCTTC
TGAAAAGGATTATCGGGATGCACTGGCGATGGTTGATTATCTCATCGAAAACCACGATGAAAGCCCATTAATCAATATAC
TGGCCGCAAAAATAGCGGAGTATGAAGACAGTAACAAACGTTTTGCTGCCTTTAACAAAGCGGTGGAAGTGATGCCAACG
GGTGTCGCCGCGCTGCGTGTATTGATGGATCAACATGGCTTGTCATATTCAGATTTGCAAGAAGAAATAGGCTCAAAGTC
ACTGATCAGCCAAATTTTTTCGGGAAAACGAGCATTAACCATTGCACATATCAAGGCACTTTCTGCCCGTTTTGATGTGA
AGCCGGATATCTTTCTCTGA

Structures

Toxin

• AlphaFold DB: D3VFW9