Detailed information of TA system TA111661

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/RelE-HTH
• Location: 3325083..3325733
• Replicon: chromosome
• Accession: NZ_CP032329
• Organism: Xenorhabdus nematophila strain YL001

Toxin (Protein)

• Gene name: higB
• Uniprot ID: N1NNR7
• Locus tag: D3790_RS15110
• Protein ID: WP_010847562.1
• Coordinates: 3325425..3325733 (-)
• Length: 103 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: D3790_RS15105
• Protein ID: WP_010847563.1
• Coordinates: 3325083..3325379 (-)
• Length: 99 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
D3790_RS15070	3320503..3321195	-	693	WP_013183086.1	envelope stress response regulator transcription factor CpxR	-
D3790_RS15075	3321348..3321848	+	501	WP_010847568.1	Spy/CpxP family protein refolding chaperone	-
D3790_RS15080	3322221..3322664	+	444	WP_010847567.1	hypothetical protein	-
D3790_RS15085	3322796..3322969	+	174	WP_038220160.1	type II toxin-antitoxin system HicA family toxin	-
D3790_RS15090	3323024..3323440	+	417	WP_010847564.1	type II toxin-antitoxin system HicB family antitoxin	-
D3790_RS15095	3323548..3324796	+	1249	Protein_2881	IS3 family transposase	-
D3790_RS15105	3325083..3325379	-	297	WP_010847563.1	helix-turn-helix domain-containing protein	Antitoxin
D3790_RS15110	3325425..3325733	-	309	WP_010847562.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
D3790_RS15120	3326079..3326978	+	900	WP_010847560.1	CDF family cation-efflux transporter FieF	-
D3790_RS15125	3327212..3328189	+	978	WP_013183083.1	6-phosphofructokinase	-
D3790_RS15130	3328492..3329478	+	987	WP_113935453.1	sulfate ABC transporter substrate-binding protein	-
D3790_RS15135	3329614..3330720	-	1107	WP_038219933.1	Fic family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-2 (1-101)

Antitoxin

• Cro/C1-type helix-turn-helix domain (39-92)

Sequences

Toxin

Protein

• Length: 103 a.a.
• Molecular weight: 11887.92 Da
• Isoelectric Point: 10.5016

>T111661 WP_010847562.1 NZ_CP032329:c3325733-3325425 [Xenorhabdus nematophila]
VYKRQVFSRERAELLTDDEFRHLQEHLLKNHEQGSTISATGGCKKIRLQREGMGKRGGVRIIYYTIIKTGMLYLLLIYPK
NKKDDLTPKQKEVLKAISNKLQ

Nucleotide

• Length: 309 bp

>T111661 NZ_CP032329:c3325733-3325425 [Xenorhabdus nematophila]
GTGTATAAGAGACAGGTATTCAGTAGGGAACGGGCAGAGTTGTTAACCGACGATGAATTTAGACACTTACAGGAACATTT
ACTAAAGAATCATGAACAAGGCAGTACCATTAGTGCTACTGGAGGATGCAAAAAGATTCGCTTGCAGCGGGAAGGAATGG
GAAAACGTGGCGGTGTCAGAATTATCTACTACACCATCATTAAAACCGGAATGCTCTATCTTCTTTTGATTTACCCTAAA
AATAAAAAAGATGACTTAACCCCAAAACAAAAAGAAGTACTTAAAGCCATCAGTAATAAATTGCAATAA

Antitoxin

Protein

• Length: 99 a.a.
• Molecular weight: 11083.80 Da
• Isoelectric Point: 7.3210

>AT111661 WP_010847563.1 NZ_CP032329:c3325379-3325083 [Xenorhabdus nematophila]
MDKKLFDDLIESMNQMVAIDKGELSVPAENIHRHRLPDVKKLRETFGLKQSEFAEAVGVSASLIQSWEQHRRTPSGSSLK
LLKMLERNPALINELSAL

Nucleotide

• Length: 297 bp

>AT111661 NZ_CP032329:c3325379-3325083 [Xenorhabdus nematophila]
ATGGACAAAAAACTTTTTGATGATTTGATTGAAAGCATGAATCAAATGGTAGCGATTGATAAAGGGGAACTTTCTGTTCC
TGCTGAAAATATTCATCGTCATCGTCTGCCTGATGTAAAAAAACTACGCGAAACCTTTGGGTTAAAGCAAAGTGAATTTG
CCGAAGCTGTTGGCGTTTCTGCATCCTTGATCCAAAGCTGGGAGCAACACCGCCGTACTCCTTCTGGTTCATCACTTAAA
TTGCTTAAAATGCTTGAACGTAATCCTGCGCTGATTAATGAATTAAGCGCGCTTTAA

Structures

Toxin

• AlphaFold DB: N1NNR7