Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/Txe-RelB
Location 76792..77327 Replicon plasmid unnamed2
Accession NZ_CP032307
Organism Enterococcus faecium strain HY07

Toxin (Protein)


Gene name yoeB Uniprot ID R2PQC0
Locus tag CTI32_RS00455 Protein ID WP_002314366.1
Coordinates 77055..77327 (+) Length 91 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID Q3Y220
Locus tag CTI32_RS00450 Protein ID WP_002297422.1
Coordinates 76792..77055 (+) Length 88 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CTI32_RS00435 72220..74469 + 2250 WP_119364661.1 alpha-galactosidase -
CTI32_RS00440 74472..75647 + 1176 WP_119364662.1 MFS transporter -
CTI32_RS00445 75650..76558 - 909 WP_119364663.1 IS982-like element ISEfm1 family transposase -
CTI32_RS00450 76792..77055 + 264 WP_002297422.1 type II toxin-antitoxin system Phd/YefM family antitoxin Antitoxin
CTI32_RS00455 77055..77327 + 273 WP_002314366.1 Txe/YoeB family addiction module toxin Toxin
CTI32_RS00460 77404..77733 + 330 WP_002292678.1 AbrB family transcriptional regulator -
CTI32_RS00465 77723..78010 + 288 WP_002292679.1 type II toxin-antitoxin system mRNA interferase toxin, RelE/StbE family -
CTI32_RS00470 78296..79153 + 858 WP_002292680.1 AAA family ATPase -
CTI32_RS00475 79154..79702 + 549 WP_002292681.1 hypothetical protein -
CTI32_RS00480 79962..80257 + 296 Protein_95 hypothetical protein -
CTI32_RS00485 80332..81012 + 681 WP_119364664.1 IS6 family transposase -
CTI32_RS00490 81057..81383 + 327 Protein_97 2-dehydro-3-deoxyphosphogluconate aldolase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - - 1..258852 258852
- inside IScluster/Tn - - 70274..81012 10738


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(9-87)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(5-69)


Sequences

Toxin        


Download         Length: 91 a.a.        Molecular weight: 10780.24 Da        Isoelectric Point: 9.9724

>T111610 WP_002314366.1 NZ_CP032307:77055-77327 [Enterococcus faecium]
MNNYSVMIKNSAKADLKKIKQSNLKPQFEKVIQTLKEDPYLPTQSFEKLKPTHEGRYSRRLNRQHRVVYKVDEDEHVVEI
YSARTHYESQ

Download         Length: 273 bp

>T111610 NZ_CP032307:77055-77327 [Enterococcus faecium]
ATGAATAACTATTCTGTGATGATTAAAAACTCGGCTAAAGCTGATTTAAAGAAAATCAAACAGTCTAATTTGAAACCTCA
ATTTGAGAAGGTTATTCAGACTTTAAAGGAAGATCCCTATCTCCCTACCCAATCATTTGAGAAGTTAAAACCTACACATG
AAGGTAGGTATTCTAGAAGATTGAATAGACAACACCGGGTTGTTTATAAGGTAGATGAAGATGAACACGTGGTTGAAATT
TATTCAGCAAGGACGCATTACGAGTCACAGTAA

Antitoxin


Download         Length: 88 a.a.        Molecular weight: 9959.22 Da        Isoelectric Point: 4.5370

>AT111610 WP_002297422.1 NZ_CP032307:76792-77055 [Enterococcus faecium]
MAKTVLNPSSARKNFYKLLKDVNENHTEIEIISERSENNAVLIGLDDWKAIKETLLLEQIGTLGVVRKREKDDSGFTNVD
EIDWDNL

Download         Length: 264 bp

>AT111610 NZ_CP032307:76792-77055 [Enterococcus faecium]
ATGGCAAAAACAGTCTTAAATCCTAGCTCAGCACGGAAAAATTTTTATAAGTTGTTGAAAGATGTAAATGAGAATCATAC
GGAAATTGAAATCATTAGTGAGCGCAGTGAAAATAATGCGGTGCTTATTGGTTTGGATGATTGGAAAGCAATCAAAGAAA
CGCTATTACTTGAGCAAATAGGCACTTTGGGTGTTGTTCGTAAGCGAGAAAAAGATGATAGTGGATTTACTAATGTTGAT
GAAATTGATTGGGATAACTTATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829F704

Antitoxin

Source ID Structure
AlphaFold DB A0A829F006

References