Detailed information of TA system TA111586

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/RelE-HTH
• Location: 11699..12284
• Replicon: plasmid p2_010055
• Accession: NZ_CP032281
• Organism: Acinetobacter sp. WCHA55

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A3F3L0I7
• Locus tag: CDG62_RS00505
• Protein ID: WP_039253786.1
• Coordinates: 11699..12019 (+)
• Length: 107 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: CDG62_RS00510
• Protein ID: WP_000369781.1
• Coordinates: 12012..12284 (+)
• Length: 91 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CDG62_RS00475	6872..8197	-	1326	WP_020899627.1	ISNCY family transposase	-
CDG62_RS00485	8388..9575	-	1188	WP_087528866.1	Tet(A)/Tet(B)/Tet(C) family tetracycline efflux MFS transporter	-
CDG62_RS00490	9641..10282	+	642	WP_004787586.1	TetR/AcrR family transcriptional regulator C-terminal domain-containing protein	-
CDG62_RS00495	10819..11133	-	315	WP_087528868.1	hypothetical protein	-
CDG62_RS00500	11272..11532	-	261	WP_087528869.1	hypothetical protein	-
CDG62_RS00505	11699..12019	+	321	WP_039253786.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
CDG62_RS00510	12012..12284	+	273	WP_000369781.1	helix-turn-helix domain-containing protein	Antitoxin
CDG62_RS00515	12377..12973	+	597	WP_087528870.1	hypothetical protein	-
CDG62_RS00520	13095..13379	+	285	WP_087528871.1	type II toxin-antitoxin system HigB family toxin	-
CDG62_RS00525	13389..14582	+	1194	WP_087528872.1	ImmA/IrrE family metallo-endopeptidase	-
CDG62_RS19265	14652..14822	-	171	WP_162904002.1	hypothetical protein	-
CDG62_RS00530	14819..15205	-	387	WP_086183207.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	msr(E) / mph(E) / tet(39)	-	1..16361	16361

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-2 (41-102)

Antitoxin

• Cro/C1-type helix-turn-helix domain (37-85)

Sequences

Toxin

Protein

• Length: 107 a.a.
• Molecular weight: 12207.00 Da
• Isoelectric Point: 7.9849

>T111586 WP_039253786.1 NZ_CP032281:11699-12019 [Acinetobacter sp. WCHA55]
MLFIETSIFTKQIKDLVSDEEYRQLQQDLLVQPDRGDLIKNGGGIRKVRCAQGNKGKSGGIRVIYYWVTEDDQIFFLVAY
PKSVKDNLTDKETAILRQLVKEQFHG

Nucleotide

• Length: 321 bp

>T111586 NZ_CP032281:11699-12019 [Acinetobacter sp. WCHA55]
ATGTTATTTATTGAAACCAGCATCTTTACCAAGCAAATTAAAGACCTTGTATCTGATGAGGAATATCGTCAACTTCAGCA
AGATCTTTTGGTACAGCCTGATAGAGGTGATCTAATCAAGAATGGCGGTGGTATTCGCAAAGTACGTTGTGCTCAAGGTA
ACAAAGGGAAAAGTGGTGGGATACGGGTGATTTATTATTGGGTCACCGAGGATGATCAGATCTTTTTCTTAGTGGCTTAT
CCAAAATCAGTAAAAGATAATTTAACAGATAAGGAAACTGCCATTCTGCGCCAACTCGTGAAGGAGCAATTTCATGGATA
A

Antitoxin

Protein

• Length: 91 a.a.
• Molecular weight: 10149.61 Da
• Isoelectric Point: 8.6582

>AT111586 WP_000369781.1 NZ_CP032281:12012-12284 [Acinetobacter sp. WCHA55]
MDNNLFDDLVASIKEAGAIKRKEVKASRVTELELPDIKEVREKTGLSQNEFAARLHISPRTLQNWEQGRRYPTGPAATLI
RILDAHPSLI

Nucleotide

• Length: 273 bp

>AT111586 NZ_CP032281:12012-12284 [Acinetobacter sp. WCHA55]
ATGGATAACAACTTATTTGATGACTTGGTTGCTTCAATCAAAGAAGCTGGAGCTATCAAACGTAAAGAAGTTAAAGCAAG
CCGAGTTACAGAACTCGAATTGCCTGATATTAAAGAAGTACGTGAAAAAACTGGCTTGAGCCAAAATGAATTTGCTGCAC
GTCTACATATTAGCCCCCGTACCCTGCAAAATTGGGAACAGGGACGACGCTATCCAACTGGACCAGCAGCTACATTGATT
CGAATTTTAGATGCTCATCCAAGTCTTATTTGA

Structures

Toxin

• AlphaFold DB: A0A3F3L0I7