Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 33225..33918 Replicon chromosome
Accession NZ_CP032279
Organism Acinetobacter sp. WCHAc010034

Toxin (Protein)


Gene name higB Uniprot ID A0A3B7PNX4
Locus tag BEN74_RS01440 Protein ID WP_068910447.1
Coordinates 33225..33515 (+) Length 97 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID A0A3B7PPZ3
Locus tag BEN74_RS01445 Protein ID WP_068910446.1
Coordinates 33517..33918 (+) Length 134 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BEN74_RS19185 28259..28522 + 264 WP_162898113.1 hypothetical protein -
BEN74_RS01410 28567..28788 + 222 WP_068910473.1 transposase -
BEN74_RS01415 29367..29882 + 516 WP_068910452.1 ClbS/DfsB family four-helix bundle protein -
BEN74_RS01420 30380..30697 + 318 WP_068910451.1 hypothetical protein -
BEN74_RS01430 31203..31958 + 756 WP_068910449.1 hypothetical protein -
BEN74_RS01435 31971..33017 + 1047 WP_068910448.1 linear amide C-N hydrolase -
BEN74_RS01440 33225..33515 + 291 WP_068910447.1 type II toxin-antitoxin system HigB family toxin Toxin
BEN74_RS01445 33517..33918 + 402 WP_068910446.1 helix-turn-helix domain-containing protein Antitoxin
BEN74_RS01450 34026..34745 - 720 WP_068910445.1 SDR family oxidoreductase -
BEN74_RS01455 35008..35592 - 585 WP_068910444.1 hypothetical protein -
BEN74_RS01460 36012..36404 + 393 WP_086374369.1 GFA family protein -
BEN74_RS01465 36745..38082 + 1338 WP_068910443.1 sorbosone dehydrogenase family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, mRNA interferase HigB

(19-90)

Antitoxin

Cro/C1-type helix-turn-helix domain

(84-131)


Sequences

Toxin        


Download         Length: 97 a.a.        Molecular weight: 11274.13 Da        Isoelectric Point: 9.8477

>T111585 WP_068910447.1 NZ_CP032279:33225-33515 [Acinetobacter sp. WCHAc010034]
MHVITHAKILEAIEKWPQAETALDGWYRIIKANDPKDFADMKHYFPAVDKVGKFHVFDIGGNKIRLIAVVMYQAKRLYIR
HVLSHKEYDKGHWKEG

Download         Length: 291 bp

>T111585 NZ_CP032279:33225-33515 [Acinetobacter sp. WCHAc010034]
ATGCATGTGATCACCCACGCCAAAATTCTGGAAGCAATCGAAAAGTGGCCGCAGGCTGAAACGGCATTAGATGGCTGGTA
TCGAATAATTAAAGCAAATGATCCTAAAGATTTTGCAGATATGAAGCATTATTTTCCTGCTGTTGATAAGGTTGGAAAAT
TTCATGTATTTGATATAGGCGGAAATAAAATCAGATTGATTGCTGTGGTTATGTATCAGGCAAAACGGCTGTATATCCGT
CATGTTCTATCTCATAAAGAGTACGATAAAGGTCATTGGAAGGAGGGTTAA

Antitoxin


Download         Length: 134 a.a.        Molecular weight: 14743.73 Da        Isoelectric Point: 4.6009

>AT111585 WP_068910446.1 NZ_CP032279:33517-33918 [Acinetobacter sp. WCHAc010034]
MNAMIKQAVDHWRYVAPLLSKPENDQDFHALIEALDELLDIAGDDETHPLIGLIHQLGDLVSAYENEHLPMPKGDGRAAL
AFLMAQQGLSQSDLPEVAAQSVISEILSGKRQLNIRHIKALSERFNVSADTFF

Download         Length: 402 bp

>AT111585 NZ_CP032279:33517-33918 [Acinetobacter sp. WCHAc010034]
ATGAATGCAATGATTAAGCAGGCGGTTGATCACTGGCGCTATGTTGCCCCATTGCTCAGCAAGCCGGAAAATGACCAGGA
CTTTCATGCGCTGATTGAGGCGCTGGATGAGCTTTTGGATATTGCCGGGGATGATGAAACGCATCCTTTAATCGGGTTAA
TCCATCAGCTTGGCGACTTGGTTTCTGCCTACGAAAATGAACACCTGCCTATGCCTAAAGGCGACGGGCGCGCAGCGCTG
GCTTTTTTGATGGCACAGCAGGGGCTGAGCCAATCTGACCTGCCCGAGGTGGCGGCGCAATCCGTCATTTCAGAGATACT
AAGCGGCAAGCGCCAGCTCAATATCCGCCATATTAAAGCGCTGTCTGAACGCTTTAATGTTTCAGCGGATACTTTTTTCT
AA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A3B7PNX4

Antitoxin

Source ID Structure
AlphaFold DB A0A3B7PPZ3

References