Detailed information of TA system TA111581

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/RelE-HTH
• Location: 2146..2731
• Replicon: plasmid p2_010034
• Accession: NZ_CP032270
• Organism: Acinetobacter sp. WCHAc010034

Toxin (Protein)

• Gene name: higB
• Uniprot ID: N9CJF7
• Locus tag: BEN74_RS00440
• Protein ID: WP_000897307.1
• Coordinates: 2146..2466 (+)
• Length: 107 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: BEN74_RS00445
• Protein ID: WP_000369781.1
• Coordinates: 2459..2731 (+)
• Length: 91 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BEN74_RS00425	1..930	+	930	WP_038343842.1	replication initiation protein RepM	-
BEN74_RS00430	998..1543	+	546	WP_068911103.1	plasmid replication DNA-binding protein	-
BEN74_RS00435	1749..2024	+	276	WP_004967304.1	hypothetical protein	-
BEN74_RS00440	2146..2466	+	321	WP_000897307.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
BEN74_RS00445	2459..2731	+	273	WP_000369781.1	helix-turn-helix domain-containing protein	Antitoxin
BEN74_RS00450	3224..4699	+	1476	WP_000052512.1	ABC-F type ribosomal protection protein Msr(E)	-
BEN74_RS00455	4755..5639	+	885	WP_000155092.1	Mph(E) family macrolide 2'-phosphotransferase	-
BEN74_RS00460	5829..6440	-	612	WP_015060246.1	recombinase family protein	-
BEN74_RS00465	6657..7517	+	861	WP_000557454.1	aminoglycoside N-acetyltransferase AAC(3)-IId	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	msr(E) / mph(E) / aac(3)-IId	-	1..14502	14502
	-	flank	IS/Tn	msr(E) / mph(E) / aac(3)-IId	-	3224..11228	8004

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-2 (41-102)

Antitoxin

• Cro/C1-type helix-turn-helix domain (37-85)

Sequences

Toxin

Protein

• Length: 107 a.a.
• Molecular weight: 12187.95 Da
• Isoelectric Point: 7.1639

>T111581 WP_000897307.1 NZ_CP032270:2146-2466 [Acinetobacter sp. WCHAc010034]
MLFIETSIFTKQIKDLVSDEEYRQLQQDLLVQPDRGDLIKNGGGIRKVRCAQGNKGKSGGIRVIYYWVTEDDQIFFLVAY
PKSVKDNLTDKETAILHQLVKEQFHG

Nucleotide

• Length: 321 bp

>T111581 NZ_CP032270:2146-2466 [Acinetobacter sp. WCHAc010034]
ATGTTATTTATTGAAACCAGCATCTTTACCAAGCAAATTAAAGACCTTGTATCTGATGAGGAATATCGTCAACTTCAGCA
AGATCTTTTGGTACAGCCTGATAGAGGTGACCTAATCAAGAATGGCGGTGGTATTCGCAAAGTACGTTGTGCTCAAGGTA
ACAAAGGGAAAAGTGGTGGGATACGGGTGATTTATTATTGGGTCACCGAGGATGATCAGATCTTTTTCTTAGTGGCTTAT
CCAAAATCAGTAAAAGATAATTTAACAGATAAGGAAACCGCCATTCTGCACCAACTCGTGAAGGAGCAATTTCATGGATA
A

Antitoxin

Protein

• Length: 91 a.a.
• Molecular weight: 10149.61 Da
• Isoelectric Point: 8.6582

>AT111581 WP_000369781.1 NZ_CP032270:2459-2731 [Acinetobacter sp. WCHAc010034]
MDNNLFDDLVASIKEAGAIKRKEVKASRVTELELPDIKEVREKTGLSQNEFAARLHISPRTLQNWEQGRRYPTGPAATLI
RILDAHPSLI

Nucleotide

• Length: 273 bp

>AT111581 NZ_CP032270:2459-2731 [Acinetobacter sp. WCHAc010034]
ATGGATAACAACTTATTTGATGACTTGGTTGCTTCAATCAAAGAAGCTGGAGCTATCAAACGTAAAGAAGTTAAAGCAAG
CCGAGTTACAGAACTCGAATTGCCTGATATTAAAGAAGTACGTGAAAAAACTGGCTTGAGCCAAAATGAATTTGCTGCAC
GTCTACATATTAGCCCCCGTACCCTGCAAAATTGGGAACAGGGACGACGCTATCCAACTGGACCAGCAGCTACATTGATT
CGAATTTTAGATGCTCACCCAAGCCTTATTTGA

Structures

Toxin

• AlphaFold DB: A0A0A8TMZ3