Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-YefM
Location 4032039..4032541 Replicon chromosome
Accession NZ_CP032265
Organism Escherichia coli strain AR_0089

Toxin (Protein)


Gene name yoeB Uniprot ID A0A7A2Z7A8
Locus tag AM468_RS21840 Protein ID WP_024705227.1
Coordinates 4032287..4032541 (+) Length 85 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID S1Q063
Locus tag AM468_RS21835 Protein ID WP_001259255.1
Coordinates 4032039..4032290 (+) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AM468_RS21800 4027217..4028284 - 1068 WP_000080128.1 bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB -
AM468_RS21805 4028284..4029354 - 1071 WP_061157916.1 histidinol-phosphate transaminase -
AM468_RS21810 4029351..4030655 - 1305 WP_061157915.1 histidinol dehydrogenase -
AM468_RS21815 4030661..4031560 - 900 WP_000131756.1 ATP phosphoribosyltransferase -
AM468_RS21825 4031706..4031756 - 51 WP_001364200.1 his operon leader peptide -
AM468_RS21835 4032039..4032290 + 252 WP_001259255.1 YoeB-YefM toxin-antitoxin system antitoxin YefM Antitoxin
AM468_RS21840 4032287..4032541 + 255 WP_024705227.1 type II toxin-antitoxin system mRNA interferase toxin YoeB Toxin
AM468_RS21845 4032624..4033448 + 825 WP_000754737.1 SDR family oxidoreductase -
AM468_RS21850 4033494..4034423 + 930 WP_000803366.1 LysR family transcriptional regulator -
AM468_RS21855 4034638..4034700 + 63 WP_010723108.1 membrane protein YoeI -
AM468_RS21860 4034690..4036048 + 1359 WP_000019194.1 putrescine/proton symporter PlaP -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(5-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-73)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 10173.54 Da        Isoelectric Point: 8.0353

>T111569 WP_024705227.1 NZ_CP032265:4032287-4032541 [Escherichia coli]
VKLIWSEESWDDYLYWQETDKRIVKKINEIIKDTRRTPFEGKGKPESLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAAC
RYHY

Download         Length: 255 bp

>T111569 NZ_CP032265:4032287-4032541 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAAATTATCAAAGATACCCGCAGAACACCATTTGAAGGTAAGGGGAAACCAGAATCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACTGATGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9307.58 Da        Isoelectric Point: 4.7954

>AT111569 WP_001259255.1 NZ_CP032265:4032039-4032290 [Escherichia coli]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Download         Length: 252 bp

>AT111569 NZ_CP032265:4032039-4032290 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACCATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTCATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACGGAAAAGGAC
ATTATTGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7A2Z7A8

Antitoxin

Source ID Structure
PDB 2A6Q
AlphaFold DB A0A7U9LNR0

References